Autocrine gastrins in colon cancer cells Up-regulate cytochrome c oxidase Vb and down-regulate efflux of cytochrome c and activation of caspase-3.
AuthorsWu H, Rao GN, Dai B, Singh P
JournalJ Biol Chem
PubMed ID10915781
'Suppression of the gastrin gene in human colon cancer cells by stably expressing antisense (AS) gastrin RNA results in significant growth suppression of AS cells. To understand mechanisms mediating the growth effects of autocrine gastrins, differential expression of transcripts by AS and control (C) clones of a representative cell line ... More
Mammalian cytochrome-c oxidase: characterization of enzyme and immunological detection of subunits in tissue extracts and whole cells.
AuthorsCapaldi RA, Marusich MF, Taanman JW
JournalMethods Enzymol
PubMed ID8592440
Complexity and tissue specificity of the mitochondrial respiratory chain.
AuthorsCapaldi RA, Halphen DG, Zhang YZ, Yanamura W
JournalJ Bioenerg Biomembr
PubMed ID2841307
There is a renewed interest in the structure and functioning of the mitochondrial respiratory chain with the realization that a number of genetic disorders result from defects in mitochondrial electron transfer. These socalled mitochondrial myopathies include diseases of muscle, heart, and brain. The respiratory chain can be fractionated into four ... More
Cytochrome C oxidase activity and oxygen tolerance.
AuthorsCampian JL, Gao X, Qian M, Eaton JW
JournalJ Biol Chem
PubMed ID17303578
Most cultured cells and intact animals die under hyperoxic conditions. However, a strain of HeLa cells that proliferates under 80% O(2), termed "HeLa-80," has been derived from wildtype HeLa cells ("HeLa-20") by selection for resistance to stepwise increases of oxygen partial pressure. The tolerance of HeLa-80 cells to hyperoxia is ... More
Subunit specific monoclonal antibodies show different steady-state levels of various cytochrome-c oxidase subunits in chronic progressive external ophthalmoplegia.
AuthorsTaanman JW, Burton MD, Marusich MF, Kennaway NG, Capaldi RA
JournalBiochim Biophys Acta
PubMed ID8611660
Monoclonal antibodies recognizing the mitochondrially encoded subunits I and II, and the nuclear-encoded subunits IV, Va, Vb and VIc of human cytochrome-c oxidase were generated. These antibodies are highly specific and allow the assessment of subunit steady-state levels in crude cell extracts and tissue sections. In the experimental human cell ... More
Variations in the subunit content and catalytic activity of the cytochrome c oxidase complex from different tissues and different cardiac compartments.
AuthorsVijayasarathy C, Biunno I, Lenka N, Yang M, Basu A, Hall IP, Avadhani NG
JournalBiochim Biophys Acta
PubMed ID9565657
The composition and activity of cytochrome c oxidase (COX) was studied in mitochondria from rat liver, brain, kidney and heart and also in different compartments of the bovine heart to see whether any correlation exists between known oxidative capacity and COX activity. Immunoblot analysis showed that the levels of ubiquitously ... More