Filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis.
AuthorsAruni AW, Roy F, Fletcher HM,
JournalInfect Immun
PubMed ID21825062
'Filifactor alocis, a Gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. There is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. To evaluate the virulence potential of F. alocis and its ability ... More
VimA-dependent modulation of acetyl coenzyme A levels and lipid A biosynthesis can alter virulence in Porphyromonas gingivalis.
AuthorsAruni AW, Lee J, Osbourne D, Dou Y, Roy F, Muthiah A, Boskovic DS, Fletcher HM,
JournalInfect Immun
PubMed ID22144476
'The Porphyromonas gingivalis VimA protein has multifunctional properties that can modulate several of its major virulence factors. To further characterize VimA, P. gingivalis FLL406 carrying an additional vimA gene and a vimA-defective mutant in a different P. gingivalis genetic background were evaluated. The vimA-defective mutant (FLL451) in the P. gingivalis ... More
Preterm human milk contains a large pool of latent TGF-ß, which can be activated by exogenous neuraminidase.
AuthorsNamachivayam K, Blanco CL, Frost BL, Reeves AA, Jagadeeswaran R, MohanKumar K, Safarulla A, Mandal P, Garzon SA, Raj JU, Maheshwari A,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID23558011
'Human milk contains substantial amounts of transforming growth factor (TGF)-ß, particularly the isoform TGF-ß2. We previously showed in preclinical models that enterally administered TGF-ß2 can protect against necrotizing enterocolitis (NEC), an inflammatory bowel necrosis of premature infants. In this study we hypothesized that premature infants remain at higher risk of ... More
Resistance of influenza viruses to neuraminidase inhibitors--a review.
AuthorsMcKimm-Breschkin JL
JournalAntiviral Res
PubMed ID10930642
Neuraminidase inhibition and viral chemotherapy.
AuthorsHaskell TH, Peterson FE, Watson D, Plessas NR, Culbertson T
JournalJ Med Chem
PubMed ID4318072
Abrogation of neuraminidase reduces biofilm formation, capsule biosynthesis, and virulence of Porphyromonas gingivalis.
AuthorsLi C, Kurniyati, Hu B, Bian J, Sun J, Zhang W, Liu J, Pan Y, Li C,
JournalInfect Immun
PubMed ID22025518
The oral bacterium Porphyromonas gingivalis is a key etiological agent of human periodontitis, a prevalent chronic disease that affects up to 80% of the adult population worldwide. P. gingivalis exhibits neuraminidase activity. However, the enzyme responsible for this activity, its biochemical features, and its role in the physiology and virulence ... More
Hydrophobic inactivation of influenza viruses confers preservation of viral structure with enhanced immunogenicity.
AuthorsRaviv Y, Blumenthal R, Tompkins SM, Humberd J, Hogan RJ, Viard M,
JournalJ Virol
PubMed ID18305038
The use of inactivated influenza virus for the development of vaccines with broad heterosubtypic protection requires selective inactivation techniques that eliminate viral infectivity while preserving structural integrity. Here we tested if a hydrophobic inactivation approach reported for retroviruses could be applied to the influenza virus. By this approach, the transmembrane ... More
A coupled enzyme assay for measurement of sialidase activity.
AuthorsAlon R, Bayer EA, Wilchek M
JournalJ Biochem Biophys Methods
PubMed ID1706371
A multi-coupled enzyme assay system for determining sialidase activity is described. Enzymes, substrates and chromogens are reacted in situ and determined spectrophotometrically in ELISA microtiter plates. Sialidase is assayed by the extent of desialylated galactose on an appropriate sialoglycoconjugate (fetuin), which is otherwise unavailable for oxidation by galactose oxidase. The ... More
The leukemoid reaction in Clostridium sordellii infection: neuraminidase induction of promyelocytic cell proliferation.
AuthorsAldape MJ, Bryant AE, Ma Y, Stevens DL
JournalJ Infect Dis
PubMed ID17492600
Life-threatening Clostridium sordellii infections have recently been reported in women undergoing therapeutic abortion, during natural childbirth, and in injection drug users. Shock, diffuse capillary leak, and a leukemoid reaction (LR) are cardinal features of these infections. The magnitude of the LR is highly correlated with mortality. We have isolated a ... More
Development of a sensitive chemiluminescent neuraminidase assay for the determination of influenza virus susceptibility to zanamivir.
AuthorsBuxton RC, Edwards B, Juo RR, Voyta JC, Tisdale M, Bethell RC
JournalAnal Biochem
PubMed ID10790313
Determination of the sensitivity of influenza viruses to neuraminidase (NA) inhibitors is presently based on assays of NA function because, unlike available cell culture methods, the results of such assays are predictive of susceptibility in vivo. At present the most widely used substrate in assays of NA function is the ... More