Galactose oxidase action on galactose containing glycolipids--a fluorescence method.
AuthorsFortelius M, Mattjus P
JournalChem Phys Lipids
PubMed ID16647698
'Features that alter the glycolipid sugar headgroup accessibility at the membrane interface have been studied in bilayer lipid model vesicles using a fluorescence technique with the enzyme galactose oxidase. The effects on oxidation caused by variation in the hydrophobic moiety of galactosylceramide or the membrane environment for galactosylceramide, monogalactosyldiacylglycerol and ... More
Physicochemical and biological characterization of targeted, nucleic acid-containing nanoparticles.
AuthorsBartlett DW, Davis ME
JournalBioconjug Chem
PubMed ID17326672
Nucleic acid-based therapeutics have the potential to provide potent and highly specific treatments for a variety of human ailments. However, systemic delivery continues to be a significant hurdle to success. Multifunctional nanoparticles are being investigated as systemic, nonviral delivery systems, and here, we describe the physicochemical and biological characterization of ... More
Distinct properties of the five UDP-D-glucose/UDP-D-galactose 4-epimerase isoforms of Arabidopsis thaliana.
AuthorsBarber C, Rösti J, Rawat A, Findlay K, Roberts K, Seifert GJ
JournalJ Biol Chem
PubMed ID16644739
Plant genomes contain genetically encoded isoforms of most nucleotide sugar interconversion enzymes. Here we show that Arabidopsis thaliana has five genes encoding functional UDP-D-glucose/UDP-D-galactose 4-epimerase (named UGE1 to UGE5). All A. thaliana UDP-d-glucose 4-epimerase isoforms are dimeric in solution, maximally active in vitro at 30-40 degrees C, and show good ... More