Amplex™ Red 黄嘌呤/黄嘌呤氧化酶检测试剂盒

Amplex Red 黄嘌呤/黄嘌呤氧化酶检测试剂盒提供了一种灵敏、简单的荧光检测方法，可用于在 100 μL 试验体积中检测纯化系统中低至 200 nM 的黄嘌呤或次黄嘌呤或低至了解更多信息

货号	数量
A22182	400 Assays

货号 A22182

价格（CNY)

6,766.00

添加至购物车

400 Assays

价格（CNY)

6,766.00

添加至购物车

Amplex Red 黄嘌呤/黄嘌呤氧化酶检测试剂盒提供了一种灵敏、简单的荧光检测方法，可用于在 100 μL 试验体积中检测纯化系统中低至 200 nM 的黄嘌呤或次黄嘌呤或低至 0.1 mU/mL 的黄嘌呤氧化酶活性。

仅供科研使用。不可用于诊断程序。

检测方法荧光

染料类型其他标记或染料

产品规格管、96 孔板

数量400 Assays

运输条件室温

适用于（应用）黄嘌呤/黄嘌呤氧化酶测定试剂盒

适用于（设备）微孔板读数仪, 分光光度计, 荧光计

产品线Amplex™

产品类型红色黄嘌呤/黄嘌呤氧化酶测定试剂盒

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I'm using an Amplex Red kit, the reagent changes color to pink almost immediately in my own Krebs-Ringer buffer but not in HBSS. Why is this?

The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H₂O₂ oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can Amplex Red Assays be performed using cell lysates?

This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H₂O₂ required for the reaction, competing with HRP (and catalase) for the substrate.

The Amplex Red Assays are best performed with either purified enzymes or extracted H₂O₂ in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.