Capillary electrophoresis of pesticides: V. Analysis of pyrethroid insecticides via their hydrolysis products labeled with a fluorescing and UV absorbing tag for laser-induced fluorescence and UV detection.
AuthorsKarcher A, El Rassi Z
JournalElectrophoresis
PubMed ID9237575
'Some representative standard pyrethroid insecticides, namely permethrin, phenotrin, cypermethrin, sanmarton and fenpropathrin, were subjected to base hydrolysis with the aim of facilitating the indirect determination of these neutral species of low water solubilities by aqueous capillary electrophoresis. This first involved the base fragmentation of the pyrethroids in alcohol buffer (pH ... More
Probing the orientation of reconstituted maltoporin channels at the single-protein level.
AuthorsDanelon C, Brando T, Winterhalter M
JournalJ Biol Chem
PubMed ID12835320
'Recently we have shown that maltoporin channels reconstituted into black lipid membranes have pronounced asymmetric properties in both ion conduction and sugar binding. This asymmetry revealed also that maltoporin insertion is directional. However, the orientation in the lipid bilayer remained an open question. To elucidate the orientation, we performed point ... More
Analysis of fluorescently labeled sugars by reversed-phase ion-pairing high-performance liquid chromatography.
AuthorsKim YS, Liu J, Han XJ, Pervin A, Linhardt RJ
JournalJ Chromatogr Sci
PubMed ID7738134
'Reducing sugars, including monosaccharides, disaccharides, and a trisaccharide, are derivatized by reductive amination with 7-amino-1,3-naphthalene disulfonic acid. Reversed-phase ion-pairing high-performance liquid chromatography is then used to separate these visibly fluorescent, charged conjugates. Isocratic elution with triethylamine-acetic acid from a phenyl column, a C18 column, and C18 and phenyl columns in ... More
Analysis of the antidiabetic drug acarbose by capillary electrophoresis.
AuthorsRethfeld I, Blaschke G
JournalJ Chromatogr B Biomed Sci Appl
PubMed ID9390736
'This study describes the derivatization of the pseudooligosaccharide acarbose and its main metabolite, component 2, with 7-aminonaphthalene-1,3-disulfonic acid (ANDS) in human urine. Their efficient separation was possible by means of capillary zone electrophoresis, using a capillary tube of fused-silica containing 100 mM triethylammonium phosphate buffer, pH 1.5. On column laser-induced ... More
Electrophoresis for the analysis of acidic oligosaccharides.
AuthorsKoketsu M, Linhardt RJ
JournalAnal Biochem
PubMed ID10906233
Recent advances in capillary electrophoresis of carbohydrates.
AuthorsEl Rassi Z, Mechref Y
JournalElectrophoresis
PubMed ID8900934
Capillary zone electrophoresis of derivatized acidic monosaccharides.
AuthorsMechref Y, el Rassi Z
JournalElectrophoresis
PubMed ID7925240
A new and specific precolumn derivatization reaction for acidic monosaccharides was introduced and evaluated in the separation and sensitive detection of carbohydrates by capillary electrophoresis. The derivatization reaction involved the attachment of sulfanilic acid (a UV absorbing tag) or 7-amino-naphthalene-1,3-disulfonic acid (a UV absorbing and fluorescing tag) via a condensation ... More
Analysis of fluorogenic Smith degradation products of 7-(1,3-disulfonaphtyl)amino-disaccharides for linkage position analysis of carbohydrates.
AuthorsMakino Y, Yonezaki K, Omichi K
JournalJ Biochem (Tokyo)
PubMed ID12473199
The linkage position of a glycosidic bond to the reducing-end residue of a pyridylamino (PA-) sugar can be determined sensitively by Smith degradation and HPLC [K. Omichi and S. Hase, (1994) J. Biochem. 115, 429-434]. With the aim of enhancing the sensitivity of this method of linkage position analysis to ... More
Capillary electrophoresis of carboxylated carbohydrates. III. Selective precolumn derivatization of glycosaminoglycan disaccharides with 7-aminonaphthalene-1,3-disulfonic acid fluorescing tag for ultrasensitive laser-induced fluorescence detection.
Authorsel Rassi Z, Postlewait J, Mechref Y, Ostrander GK
JournalAnal Biochem
PubMed ID9025945
Eight different glycosaminoglycan-derived disaccharides were selectively labeled via their carboxylic acid group with 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) by a condensation reaction between the amino group of ANDSA and the carboxylic acid group of the saccharides in the presence of water-soluble carbodiimide. This derivatization reaction yielded stable derivatives with percentage yields as ... More
Capillary electrophoresis of carboxylated carbohydrates. Part 2. Selective precolumn derivatization of sialooligosaccharides derived from gangliosides with 7-aminonaphthalene-1,3-disulfonic acid fluorescing tag.
AuthorsMechref Y, Ostrander GK, el Rassi Z
JournalElectrophoresis
PubMed ID8529621
The most suitable conditions for selective precolumn derivatization of sialooligosaccharides, derived from gangliosides, with 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) and the subsequent separation of the derivatives by capillary electrophoresis are described. ANDSA-sialooligosaccharide derivatives, which fluoresce at 420 nm when excited at 315 nm, were readily detected in capillary electrophoresis using an on-column ... More
High-performance liquid-phase separation of glycosides. III. Determination of total glucosinolates in cabbage and rapeseed by capillary electrophoresis via the enzymatically released glucose.
AuthorsKarcher A, Melouk HA, El Rassi Z
JournalAnal Biochem
PubMed ID9918659
A selective and sensitive method for the determination of total glucosinolates (GSs) in plant extracts by capillary electrophoresis-laser-induced fluorescence (LIF) detection was developed. It was based on the enzymatically released glucose from glucosinolates in the presence of the hydrolyzing enzyme myrosinase. The released glucose was converted to gluconic acid (GA) ... More
Capillary electrophoresis of carboxylated carbohydrates. IV. Adjusting the separation selectivity of derivatized carboxylated carbohydrates by controlling the electrolyte ionic strength at subambient temperature and in the absence of electroosmotic flow.
AuthorsMechref Y, Ostrander GK, el Rassi Z
JournalJ Chromatogr A
PubMed ID9463906
The effect of the ionic strength of the running electrolyte on selectivity and resolution of 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) derivatives of carboxylated monosaccharides and sialooligosaccharides derived from gangliosides was evaluated in capillary electrophoresis in the absence of electroosmotic flow and at subambient temperature. The acidic saccharides used in this study were ... More
Capillary electrophoresis of herbicides: IV. Evaluation of octylmaltopyranoside chiral surfactant in the enantiomeric separation of fluorescently labeled phenoxy acid herbicides and their laser-induced fluorescence detection.
AuthorsMechref Y, el Rassi Z
JournalElectrophoresis
PubMed ID9080129
A novel chiral nonionic surfactant, namely octyl-b-D-maltopyranoside (OM), was evaluated in chiral capillary electrophoresis of fluorescently labeled phenoxy acid herbicides. The labeling of the analytes with 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) permitted a concentration detection limit of 5 x 10-10 M using laser-induced fluorescence detection. This limit of detection allowed the determination ... More
Capillary electrophoresis of carboxylated carbohydrates. I. Selective precolumn derivatization of gangliosides with UV absorbing and fluorescent tags.
AuthorsMechref Y, Ostrander GK, el Rassi Z
JournalJ Chromatogr A
PubMed ID7735460
We demonstrate that the precolumn derivatization reaction, recently introduced by our laboratory for the selective labeling of carboxylated monosaccharides, can be readily transposed to other glycoconjugates containing carboxylated sugar residues, namely sialogangliosides. The selective derivatization reaction described here involved the attachment of sulfanilic acid (a UV-absorbing tag) or 7-aminonaphthalene-1,3-disulfonic acid ... More
Electrically driven microseparation methods for pesticides and metabolites: IV. Effects of the nature of fluorescent labels on the enantioseparation of pesticides and their degradation products by capillary zone electrophoresis with UV and laser-induced fluorescence detection.
AuthorsKarcher A, El Rassi Z
JournalElectrophoresis
PubMed ID10879964
Three different fluorescent tags, namely 5-aminonaphthalene-1-sulfonic acid (ANSA), 7-aminonaphthalene-1,3-disulfonic acid (ANDSA), and 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) were evaluated in the precolumn derivatization of some chiral phenoxy acid herbicides, some chiral transformation products of pyrethroid insecticides, and in their subsequent enantiomeric separation by capillary electrophoresis (CE). The electrolyte systems consisted of sodium ... More