Effect of salt and membrane fluidity on fluorophore motions of a gramicidin C derivative.
AuthorsTeng Q, Koeppe RE, Scarlata SF
JournalBiochemistry
PubMed ID1714298
We have used fluorescence spectroscopy to investigate the effect of salt and membrane fluidity on the rotational motion of a 5-(dimethylamino)naphthalene-1-sulfonyl (dansyl) derivative of gramicidin C (dansyl-gC) in dimyristoylphosphatidylcholine bilayers, under conditions where the peptide is a formyl-NH to formyl-NH terminal dimer, and in octyl glucoside micelles, where the peptide ... More
Mechanism and kinetics of merocyanine 540 binding to phospholipid membranes.
AuthorsVerkman AS
JournalBiochemistry
PubMed ID3651436
The physicochemical mechanism for merocyanine 540 (M540) binding to unilamellar phosphatidylcholine (PC) vesicles was examined by steady-state and dynamic fluorescence and fluorescence stopped-flow methods. At 530-nm excitation, aqueous M540 has an emission peak at 565 nm, which red shifts to 580 nm with formation of membrane-bound monomers (M); bound dimers ... More
Study of a fatty acid binding site of interphotoreceptor retinoid-binding protein using fluorescent fatty acids.
AuthorsPutilina T, Sittenfeld D, Chader GJ, Wiggert B
JournalBiochemistry
PubMed ID8466918
'Interphotoreceptor retinoid-binding protein (IRBP) is a 140-kDa glycolipoprotein which constitutes about 70% of the soluble protein of the retinal interphotoreceptor matrix. Much is known concerning its role in the transport of retinoids between photoreceptor cells and pigment epithelium but little is known about its interactions with lipids. Here we have ... More
Determination of the location of fluorescent probes attached to fatty acids using parallax analysis of fluorescence quenching: effect of carboxyl ionization state and environment on depth.
AuthorsAbrams FS, Chattopadhyay A, London E
JournalBiochemistry
PubMed ID1606156
'In this report, parallax analysis of fluorescence quenching (see the preceding paper in this issue) was used to determine the location (depth) of anthroyloxy and carbazole probes attached to model membrane inserted fatty acids. A monotonic increase in depth was found as the number of carbon atoms between the attachment ... More
Stretch sensitivity of transmembrane mobility of hydrogen peroxide through voids in the bilayer. Role of cardiolipin.
AuthorsMathai JC, Sitaramam V
JournalJ Biol Chem
PubMed ID8027032
'Availability of voids for diffusion of quinone in the membrane was shown to be the rate-limiting step in electron transport in mitochondria and chloroplasts (Mathai, J. C., Sauna, Z. E., John, O., and Sitaramam, V (1993) J. Biol. Chem. 268, 15442-15454). The primary role of voids in these diffusion-controlled reactions ... More
Nonmonotonic alterations in the fluorescence anisotropy of polar head group labeled fluorophores during the lamellar to hexagonal phase transition of phospholipids.
AuthorsHan X, Gross RW
JournalBiophys J
PubMed ID1420879
'The temperature dependence of the fluorescence anisotropy of polar head group labeled fluorophores (i.e., N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L- alpha-phosphatidylethanolamine or N-(lissamine rhodamine B sulfonyl)dipalmitoyl-L-alpha-phosphatidylethanol- amine) incorporated into multiple phosphatidylethanolamine molecular species was parabolic, possessing minima (dr/dT = 0) that precisely correlated with the respective lamellar (L alpha) to hexagonal (HII) phase transition temperature ... More
Interaction of antioxidants with depth-dependent fluorescence quenchers and energy transfer probes in lipid bilayers.
AuthorsHinzmann JS, McKenna RL, Pierson TS, Han F, Kezdy FJ, Epps DE
JournalChem Phys Lipids
PubMed ID1423807
'Three fluorescent, lipophilic, heterocyclic antioxidants were incorporated into lipid bilayers and exposed to depth-dependent nitroxyl fatty acid quenchers. The Stern-Volmer plots curved upward at low quencher concentrations. Quantitative analysis of the results showed that this behavior is consistent with complex formation between quencher and fluorescent antioxidant, where the complex is ... More
Calibration of the parallax fluorescence quenching method for determination of membrane penetration depth: refinement and comparison of quenching by spin-labeled and brominated lipids.
AuthorsAbrams FS, London E
JournalBiochemistry
PubMed ID1606155
'We previously introduced the "parallax" method, which uses fluorescence quenching by spin-labeled lipids in order to measure the depth of molecules within a membrane [Chattopadhyay, A., & London, E. (1987) Biochemistry 26, 39-45]. In this report the accuracy of this method is established by comparison of spin-label quenching to that ... More
An assessment of the fluidity gradient of the lipid bilayer as determined by a set of n-(9-anthroyloxy) fatty acids (n = 2, 6, 9, 12, 16).
AuthorsTilley L, Thulborn KR, Sawyer WH
JournalJ Biol Chem
PubMed ID429303
'The rotational behavior of a set of n-(9-anthroyloxy) fatty acid fluorescent probes is examined in two liquid paraffins and in liposomes composed of dipalmitoyl phosphatidylcholine. As has been observed with other membrane fluorescent probes (Hare, F., and Lussan, C. (1977) Biochim. Biophys. Acta 467, 262-272), the degree of fluorescence depolarization ... More
Dibucaine interaction with phospholipid vesicles. A resonance energy-transfer study.
AuthorsCoutinho A, Costa J, Faria JL, Berberan-Santos MN, Prieto MJ
JournalEur J Biochem
PubMed ID2338082
'Resonance energy transfer between a local anaesthetic, dibucaine (donor) and a set of functionalized probes [n-(9-anthroyloxy)stearic acids, n = 2, 3, 6, 7, 9 and 12 and 16-(9-anthroyloxy)palmitic acid] (acceptors) was found to be an efficient process with a critical radius of transfer Ro = 2.1 nm, this interaction being ... More
Tryptophan imaging of membrane proteins.
AuthorsKleinfeld AM
JournalBiochemistry
PubMed ID4016088
'A theoretical analysis of resonance energy transfer between protein tryptophan and the n-(9-anthroyloxy) (AO) fatty acid probes has been carried out to evaluate its potential use in determining the tryptophan distribution in membrane proteins. The Förster theory for two-dimensional energy transfer was formulated to calculate multiple donor (tryptophan) transfer efficiencies ... More
Effects of ethanol on the Escherichia coli plasma membrane.
AuthorsDombek KM, Ingram LO
JournalJ Bacteriol
PubMed ID6360997
'The effects of ethanol on the fluidity of Escherichia coli plasma membranes were examined by using a variety of fluorescent probes: 1,6-diphenyl-1,3,5-hexatriene, perylene, and a set of n-(9-anthroyloxy) fatty acids. The anthroyloxy fatty acid probes were used to examine the fluidity gradient across the width of the plasma membrane and ... More
Decreased membrane deformability in Melanesian ovalocytes from Papua New Guinea.
AuthorsSaul A, Lamont G, Sawyer WH, Kidson C
JournalJ Cell Biol
PubMed ID6715408
'We examined the ability of Melanesian ovalocytes from Papua New Guinea to be deformed in order to probe the resistance of these cells to invasion by several species of malaria parasite. We found ovalocytes were refractile to drug-induced endocytosis, that they formed abnormal rouleaux, showed reduced deformability when aspirated into ... More
Uptake of n-(9-anthroyloxy) fatty acid fluorescent probes into lipid bilayers.
'A description in thermodynamic terms is given of ligand-membrane interaction which may occur by either or both a binding and a partition process. Results obtained by fluorescence enhancement and polarization techniques on the uptake of n-(9-anthroyloxy) fatty acids by phospholipid bilayers are analysed to show that binding rather than partition ... More
The interaction of local anaesthetics with synthetic phospholipid bilayers.
AuthorsSikaris KA, Sawyer WH
JournalBiochem Pharmacol
PubMed ID7138557
'The preferred position and orientation of two local anaesthetics in lipid bilayers has been determined using fluorescence quenching techniques. The aromatic amine of tetracaine and butesin quenches the fluorescence of a series of n-(9-anthroyloxy) fatty acids (n = 2,6,9,12,16) which place a fluorophore at a graded series of depths from ... More
The use of n-(9-anthroyloxy) fatty acids to determine fluidity and polarity gradients in phospholipid bilayers.
AuthorsThulborn KR, Tilley LM, Sawyer WH, Treloar FE
JournalBiochim Biophys Acta
PubMed ID508741
'A set of n-(9-anthroyloxy) fatty acid probes (n = 2, 6, 9, 12) have been used to examine gradients in fluorescence polarization, lifetime (tau F), relative quantum yield (phi rel) and positions of emission maxima (lambda max) through bilayers composed of synthetic phospholipids. The fluorophores of these probes report the ... More
Properties of phosphatidylcholine bilayers as revealed by mixed-acyl phospholipid fluorescent probes containing n-(9-anthroyloxy) fatty acids.
AuthorsMason JT
JournalBiochim Biophys Acta
PubMed ID8075146
'A series of five mixed-acyl phosphatidylcholine (PC) fluorescent probes having the structure 1-caproyl-2-(n-(9-anthroyloxy)-acyl)-sn-glycero-3-phosphocholine, where the sn-2 anthroyloxy-labeled acyl chain is stearic acid (n = 2,6,9,12) or palmitic acid (n = 16), have been prepared. These probes have been used to study the thermal behavior and transbilayer organization of 1,2-distearoyl-PC (C(18)C(18)PC), ... More
Comparison of lipid binding and transfer properties of two lipid transfer proteins from plants.
AuthorsGuerbette F, Grosbois M, Jolliot-Croquin A, Kader JC, Zachowski A
JournalBiochemistry
PubMed ID10571986
'Plant lipid transfer proteins (LTPs) are soluble proteins which are characterized by their in vitro ability to transfer phospholipids between two membranes. We have compared the functional properties of two LTPs purified from maize and wheat seeds knowing that, despite a high degree of sequence identity, the two proteins exhibit ... More
Erythrocyte, platelet and polymorphonuclear leukocyte membrane dynamic properties in essential hypertension.
AuthorsCaimi G
JournalClin Hemorheol Microcirc
PubMed ID9356784
'In subjects with essential hypertension we evaluated, respectively, the red cell membrane protein lateral mobility (obtained marking intact red blood cells with pyrene-3-maleimide (3-PM)), the erythrocyte membrane fluidity (obtained marking intact erythrocytes with 10-(1-pyrene) decanoic acid), the red cell membrane transverse fluidity gradient (obtained marking intact red blood cells with ... More
Effect of fluorophore linkage position of n-(9-anthroyloxy) fatty acids on probe distribution between coexisting gel and fluid phospholipid phases.
AuthorsHuang NN, Florine-Casteel K, Feigenson GW, Spink C
JournalBiochim Biophys Acta
PubMed ID3349074
'The quenching of probe fluorescence by spin-labeled phospholipid has been used to determine the distribution of a series of n-(9-anthroyloxy) fatty acids between coexisting gel and fluid liquid-crystal phases in multilamellar phospholipid vesicles. The phase distribution ratio in every case is found to favor the fluid lipid phase, but is ... More
Erythrocyte membrane fluidity in malignant hyperthermia.
AuthorsCooper P, Meddings JB
JournalBiochim Biophys Acta
PubMed ID1932056
'Malignant hyperthermia (MH) is a rare but serious complication of general anesthesia that potentially carries a high mortality and morbidity. It is associated with excessive release of calcium into skeletal muscle following exposure to certain drugs, including the volatile general anesthetics. Since these are recognized membrane fluidizing agents it has ... More
A microviscosity barrier in the lipid bilayer due to the presence of phospholipids containing unsaturated acyl chains.
AuthorsThulborn KR, Treloar FE, Sawyer WH
JournalBiochem Biophys Res Commun
PubMed ID656103
Properties and the locations of a set of fluorescent probes sensitive to the fluidity gradient of the lipid bilayer.
AuthorsThulborn KR, Sawyer WH
JournalBiochim Biophys Acta
PubMed ID678539
The synthesis and properties of a set of four fluorescent probes (n-(9-anthroyloxy) fatty acids, n = 2, 6, 9, 12) sensitive to the fluidity gradient of the lipid bilayer are described. Fluorescent quenching experiments show that the probes locate at a graded series of depths in the bilayer. A fifth ... More
Fluorescence quenching of a series of membrane probes measured in living cells by flow cytometry.
AuthorsCollins JM, Grogan WM
JournalCytometry
PubMed ID2036918
The transverse location normal to the bilayer surface of a series of n-(9-anthroyloxy) fatty acid probes, where n = 2, 3, 6, 7, 9, 12, and 16, was determined by fluorescence quenching measurements with a flow cytometer. We show that the anthroyloxy moieties of the probes locate at a graded ... More
The transverse organisation of ubiquinones in mitochondrial membranes as determined by fluorescence quenching. Evidence for a two-site model.
AuthorsChatelier RC, Sawyer WH
JournalEur Biophys J
PubMed ID3987642
The transverse organisation of ubiquinone in mitochondrial membranes was investigated by quenching a set of fluorescent fatty acids. We show that the fluorescent moiety of the probes is located at a graded series of depths in the mitochondrial membrane. The probes sense the characteristics of the lipid phase and do ... More
Perturbation of phospholipid bilayers by DDT.
AuthorsChefurka W, Chatelier RC, Sawyer WH
JournalBiochim Biophys Acta
PubMed ID3801467
The localization of the effects of DDT (5-50 mol%) addition on the acyl chain dynamics in unilamellar vesicles of two phosphatidylcholines (DPPC and egg PC) has been investigated by steady-state fluorescence polarization of a series of n-(9-anthroyloxy) fatty acids (n = 2, 6, 9, 12 and 16) whose fluorophore is ... More
Spectroscopic investigations on the binding site of bovine hepatic fatty acid binding protein. Evidence for the existence of a single binding site for two fatty acid molecules.
AuthorsKeuper HJ, Klein RA, Spener F
JournalChem Phys Lipids
PubMed ID4064218
The hydrophobic region of the binding site of a bovine fatty acid binding protein (pI 7.0-FABP) has been characterized using fluorescence and circular dichroism (CD) spectroscopy. Blue-shifts of fluorescence emission maxima and increased lifetimes of naphthylamine dyes, anthroyloxy-fatty acids, pyrene nonanoic acid and trans-parinaric acid indicated a hydrophobic interaction with ... More
Membrane fluidity and lipid composition of rat small intestinal brush-border membranes during postnatal maturation.
AuthorsHübner C, Lindner SG, Stern M, Claussen M, Kohlschütter A
JournalBiochim Biophys Acta
PubMed ID3349076
Fluidity and lipid composition of rat small intestinal brush-border membranes (BBM) were studied during maturation in five age groups: newborns, sucklings (1-3 weeks), weaned (4-6 weeks), juveniles (8-10 weeks), and adults (12 weeks). Brush-border membrane fluidity was measured by steady-state fluorescence polarization. Fluorescent probes used were: 1,6-diphenyl-1,3,5-hexatriene, 1-(4-trimethylammonium)phenyl)-6-phenyl-1,3,5-hexatriene, and a ... More
Comparison of fluorescence energy transfer and quenching methods to establish the position and orientation of components within the transverse plane of the lipid bilayer. Application to the gramicidin A--bilayer interaction.
AuthorsHaigh EA, Thulborn KR, Sawyer WH
JournalBiochemistry
PubMed ID89867
Fluorescence quenching and resonance energy transfer methods have been used to investigate the position of fluorophores in the lateral and transverse planes of the lipid bilayer. A series of n-(9-anthroyloxy) fatty acids (n = 2, 6, 9, and 12) have been used as energy-transfer acceptors so that apparent transfer distances ... More
Fluorescence polarization of six membrane probes in embryonal carcinoma cells after differentiation as measured on a FACS II cell sorter.
AuthorsSchaap GH, de Josselin de Jong JE, Jongkind JF
JournalCytometry
PubMed ID6201328
Fluorescence polarization measurements on a FACS II cell sorter were compared with static measurements on a spectrofluorimeter using calibration solutions and Hoechst 33258-labeled cells. For the flow cytometric measurements on the FACS we used a pseudodepolarizer for normalization of the output of the two photomultipliers. The results showed that fluorescein ... More
Energy-transfer study of cytochrome b5 using the anthroyloxy fatty acid membrane probes.
AuthorsKleinfeld AM, Lukacovic MF
JournalBiochemistry
PubMed ID4016089
Resonance energy transfer was used to study the structure of cytochrome b5 and its nonpolar segment reconstituted into sonicated vesicles of dimyristoylphosphatidylcholine. The n-(9-anthroyloxy) (AO) fatty acid probes were added to these vesicles, and energy-transfer measurements were carried out between tryptophan and AO, tryptophan and the heme moiety of cytochrome ... More
Lipid permeability of the intestinal microvillus membrane may be modulated by membrane fluidity in the rat.
AuthorsMeddings JB
JournalBiochim Biophys Acta
PubMed ID2765545
Fatty acids and cholesterol permeate across the intestinal microvillus membrane at rates dictated by the hydrophobicity of the permeating lipid and the permeability properties of the microvillus membrane. A theory has evolved suggesting that the chemical composition and physical properties of the microvillus membrane are important in determining microvillus membrane ... More
Consequences of omega -6-oleate desaturase deficiency on lipid dynamics and functional properties of mitochondrial membranes of Arabidopsis thaliana.
AuthorsCaiveau O, Fortune D, Cantrel C, Zachowski A, Moreau F
JournalJ Biol Chem
PubMed ID11104757
We probed the role of the polyunsaturated fatty acids on the dynamic and functional properties of mitochondrial membranes using the fad2 mutant of Arabidopsis thaliana, deficient in omega-6-oleate desaturase. In mitochondria of this mutant, the oleic acid content exceeded 70% of the total fatty acids, and the lipid/protein ratio was ... More
Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids.
AuthorsArora A, Byrem TM, Nair MG, Strasburg GM
JournalArch Biochem Biophys
PubMed ID10620328
The polyphenolic structures of flavonoids and isoflavonoids confer them with the ability to scavenge free radicals and to chelate transition metals, a basis for their potent antioxidant abilities. Another possible contributory mechanism toward their antioxidant activities is their ability to stabilize membranes by decreasing membrane fluidity. In this study, the ... More
Shape of the fluidity gradient in the plasma membrane of living HeLa cells.
AuthorsCollins JM, Dominey RN, Grogan WM
JournalJ Lipid Res
PubMed ID2324646
The shape of the fluidity gradient of the outer hemi-leaflet of the plasma membrane of living HeLa cells was determined using a series of n-(9-anthroyloxy) fatty acid probes where n = 2, 3, 6, 7, 9, 10, 11, 12, and 16. Fluorescence uptake and steady-state anisotropy values were obtained with ... More
Interaction of alpha-tocopherol with fatty acids in membranes and ethanol.
AuthorsStillwell W, Ehringer W, Wassall SR
JournalBiochim Biophys Acta
PubMed ID1586661
The techniques of fluorescence polarization, ultraviolet light absorbance and fluorescence quenching by acrylamide are used to probe the structural role of alpha-tocopherol in phospholipid bilayers. Using 1,6-diphenyl-1,3,5-hexatriene (DPH) and a series of (anthroyloxy)stearic acid (AS) fluorescence probes, alpha-tocopherol is shown to increase fluidity and decrease order of gel state bilayers, ... More
Fatty acid binding sites of rodent adipocyte and heart fatty acid binding proteins: characterization using fluorescent fatty acids.
AuthorsWootan MG, Bass NM, Bernlohr DA, Storch J
JournalBiochemistry
PubMed ID2248947
Murine adipocyte and rat heart fatty acid binding proteins (FABP) are closely related members of a family of cytosolic proteins which bind long-chain free fatty acids (ffa). The physical and chemical characteristics of the fatty acid binding sites of these proteins were studied using a series of fluorescent analogues of ... More
Extension of the parallax analysis of membrane penetration depth to the polar region of model membranes: use of fluorescence quenching by a spin-label attached to the phospholipid polar headgroup.
AuthorsAbrams FS, London E
JournalBiochemistry
PubMed ID8399232
The parallax method is a method by which the depth of fluorescent molecules within a membrane is calculated from the ratio of quenching induced by two spin-labeled phospholipids at different depths. In this report, the method is extended to measurements of depth in the polar headgroup region of the membrane ... More
Effect of diethylstilbestrol and related compounds on the Ca(2+)-transporting ATPase of sarcoplasmic reticulum.
Diethylstilbestrol is a potent inhibitory agent of the Ca(2+)-ATPase activity of sarcoplasmic reticulum membranes. Other structurally related molecules, such as dienestrol or hexestrol having hydroxyl groups at para positions of the two benzene rings produce similar effects. The absence or derivatization of the hydroxyl groups as occurs with trans-stilbene or ... More
Localization of cyanine dye binding to brush-border membranes by quenching of n-(9-anthroyloxy) fatty acid probes.
AuthorsCabrini G, Verkman AS
JournalBiochim Biophys Acta
PubMed ID3778893
The location and orientation of 3,3'-dipropylthiodicarbocyanine (diS-C3-(5)) binding sites in renal brush-border membrane vesicles was examined from the quenching of n-(9-anthroyloxy) fatty acid (n-AS) fluorescence. Based on previous kinetic studies (Cabrini, G. and Verkman, A.S. (1986) J. Membrane Biol. 90, 163-175) monomeric aqueous diS-C3-(5) binds to brush-border membrane vesicles (BBMV) ... More
Plasma membrane fluidity gradients of human peripheral blood leukocytes.
AuthorsCollins JM, Scott RB, Grogan WM
JournalJ Cell Physiol
PubMed ID2365745
The shape of the fluidity gradient of the outer hemileaflet of the plasma membrane of normal, living, human white blood cells was determined using a series of n-(9-anthroyloxy) fatty acid probes where n = 2, 3, 6, 7, 9, 11, 12, and 16, to establish a baseline for future studies ... More
The interaction of alpha-tocopherol and homologues with shorter hydrocarbon chains with phospholipid bilayer dispersions. A fluorescence probe study.
AuthorsKagan VE, Quinn PJ
JournalEur J Biochem
PubMed ID3345752
The intrinsic fluorescence of tocopherol homologues with hydrocarbon chains ranging from 1 carbon (C1) to 16 carbons (alpha-tocopherol, C16) and their ability to quench the fluorescence of 9-anthroyloxy derivatives of fatty acids with the fluorophore located at different positions in the hydrophobic domain of phospholipid bilayers has been used to ... More
Transfer of long-chain fluorescent free fatty acids between unilamellar vesicles.
AuthorsStorch J, Kleinfeld AM
JournalBiochemistry
PubMed ID3707905
Movement of free fatty acids (ffa) between small unilamellar vesicles (SUV) was studied by measuring the transfer of fluorescent n-(9-anthroyloxy)-labeled analogues (AOffa) between donor and acceptor vesicles. Donors were composed of egg phosphatidylcholine (PC) loaded with 1-2 mol % AOffa, and acceptors were egg PC containing 10-12 mol % N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine ... More
Comparisons of steady-state anisotropy of the plasma membrane of living cells with different probes.
AuthorsCollins JM, Grogan WM
JournalBiochim Biophys Acta
PubMed ID1878371
We have used an extended Perrin equation which was in agreement with literature data for steady-state anisotropy (rSS) for a wide variety of artificial and isolated biological membranes labeled with various probes (Van der Meer et al. (1986) Biochim. Biophys. Acta 854, 38-44 to obtain the static component (r infinity) ... More
Fluorescence spectroscopy of 9-anthroyloxy fatty acids in solvents.
AuthorsGarrison MD, Doh LM, Potts RO, Abraham W
JournalChem Phys Lipids
PubMed ID8033287
A series of anthroyloxy fatty acid (AF) fluorescent probes, with the anthroyloxy group covalently linked at various positions along the alkyl chain, were studied in solvents exhibiting a wide range of polarity and hydrogen-bond donor (Hd) and acceptor (Ha) ability. These probes were sensitive to the solvent polarity as reflected ... More
Association of cytosol hemoglobin with the membrane in intact erythrocytes.
AuthorsEisinger J, Flores J, Salhany JM
JournalProc Natl Acad Sci U S A
PubMed ID6804940
The problem of demonstrating hemoglobin binding to the erythrocyte membrane in intact cells was approached by observing the quenching of fluorescent membrane probes by hemoglobin as a function of pH. This quenching was studied by measuring fluorescence intensities and decay rates of membrane-bound donors by both right-angle and front-face fluorometry. ... More
Decreased binding of HIV-1 and vasoactive intestinal peptide following plasma membrane fluidization of CD4+ cells by phenytoin.
AuthorsLehr HA, Zimmer JP, Hübner C, Ballmann M, Hachmann W, Vogel W, Baisch H, Hartter P, Albani M, Kohlschütter A
JournalVirology
PubMed ID1978437
Plasma membrane fluidity of intact peripheral blood lymphocytes (PBL) of phenytoin-treated nonepileptic patients and phenytoin-treated CD4+ lymphoid cells H9 and K37 was determined by fluorescence anisotropy measurements. Anisotropy values of the membrane probe 6-(9-anthroyloxy) stearic acid were decreased in all cell types as compared with controls, indicating increased plasma membrane ... More
Nonpolar environment of tryptophans in erythrocyte water channel CHIP28 determined by fluorescence quenching.
AuthorsFarinas J, Van Hoek AN, Shi LB, Erickson C, Verkman AS
JournalBiochemistry
PubMed ID8218257
CHIP28 is an abundant water-transporting protein in erythrocytes, kidney proximal tubule, and other fluid-transporting tissues. To determine the environment of the four tryptophans in CHIP28, fluorescence spectra and quenching by polar and nonpolar compounds were measured in stripped human erythrocyte membranes containing CHIP28 and in proteoliposomes reconstituted with purified CHIP28; ... More
Determination of partition and lateral diffusion coefficients of ubiquinones by fluorescence quenching of n-(9-anthroyloxy)stearic acids in phospholipid vesicles and mitochondrial membranes.
AuthorsFato R, Battino M, Degli Esposti M, Parenti Castelli G, Lenaz G
JournalBiochemistry
PubMed ID3730366
The quenching of fluorescence of n-(9-anthroyloxy)stearic acids and other probes by different ubiquinone homologues and analogues has been exploited to assess the localization and lateral mobility of the quinones in lipid bilayers of model and mitochondrial membranes. The true bimolecular collisional quenching constants in the lipids together with the lipid/water ... More
Lipid domains of mycobacteria studied with fluorescent molecular probes.
AuthorsChristensen H, Garton NJ, Horobin RW, Minnikin DE, Barer MR
JournalMol Microbiol
PubMed ID10200973
The complex mycobacterial cell envelope is recognized as a critical factor in our failure to control tuberculosis, leprosy and other non-tuberculous pathogens. Although its composition has been extensively determined, many details regarding the organization of the envelope remain uncertain. This is particularly so for the non-covalently bound lipids, whose natural ... More