人神经干细胞免疫细胞化学试剂盒
人神经干细胞免疫细胞化学试剂盒
Invitrogen™

人神经干细胞免疫细胞化学试剂盒

Molecular Probes™ 人神经干细胞免疫细胞化学试剂盒可对人神经干细胞 (NSC) 的4种常见标志物进行便捷的图像分析：巢蛋白、PAX6、SOX1 和 SOX2。这种完整的免疫细胞化学了解更多信息
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货号数量
A2435420 Tests
货号 A24354
价格（CNY)
12,640.00
Each
添加至购物车
数量:
20 Tests
价格（CNY)
12,640.00
Each
添加至购物车
Molecular Probes™ 人神经干细胞免疫细胞化学试剂盒可对人神经干细胞 (NSC) 的4种常见标志物进行便捷的图像分析：巢蛋白、PAX6、SOX1 和 SOX2。这种完整的免疫细胞化学 (ICC) 试剂盒包括一套优化的一抗和二抗、细胞核 DNA 染色剂以及完成染色方案所需的全部缓冲液。

可轻松扩展至任何培养板、培养皿或玻片规格，人神经干细胞免疫细胞化学试剂盒试剂盒所含试剂使用 50 µL 染色体积足以对20份样品进行染色。

•使用高特异性抗体确认关键神经干细胞标志物的表达
•一次测量多个标志物，即可获得更多样品信息
•轻松生成漂亮的多通路图像
•使用一套完整的优化 ICC 试剂可靠处理样品

确认 NSC 标志物的表达
试剂盒中包含的抗体是为实现 NSC ICC 分析的高性能精心挑选的。这些抗体非常适合使用 Gibco™ PSC 神经诱导培养基对产生的 NSC 进行表征。

获取更多样品信息
节省时间和珍贵样品。此试剂盒中的抗体组合设计用于一次同时评估两种标志物（例如巢蛋白 & SOX2 或 SOX1 & PAX6）以及细胞核 DNA 染色。

生成漂亮的多通路图像
利用明亮、光稳定的 Alexa Fluor™ 染料的卓越性能，此试剂盒能够使用常见的蓝色、绿色和橙色/红色滤光片组采集 NSC 标志物表达的漂亮多通路图像。

可靠地处理样品
预检测的固定、透化、封闭和洗涤缓冲液可以使您专注于回答科学问题，而不是花费时间确定试剂兼容性。
仅供科研使用。不可用于诊断程序。
规格
产品类型免疫细胞化学试剂盒
数量20 Tests
偶联物Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594
Unit SizeEach
内容与储存
包含：
  • 20 µL 山羊抗人 SOX1 (50X)
  • 20 µL 兔抗人 SOX2 (50X)
  • 20 µL 小鼠抗人巢蛋白 (50X)
  • 20 µL 兔抗人 PAX6 (50X)
  • 20 µL Alexa Fluor™ 488 抗小鼠 (250X)
  • 20 µL Alexa Fluor™ 488 抗山羊 (250X)
  • 40 µL Alexa Fluor™ 555 抗兔 (250X)
  • 40 µL Alexa Fluor™ 594 抗兔 (250X)
  • 1小瓶 NucBlue™ 固定细胞染色 ReadyProbes™ 试剂 (1X)
  • 10 mL 固定液 (1X)
  • 10 mL 透化溶液 (1X)
  • 20 mL 封闭溶液 (1X)
  • 20 mL 洗涤缓冲液 (10X)

    • 常见问题解答 (FAQ)

    Can I purchase the primary antibodies from the Human Neural Stem Cell Immunocytochemistry Kit (Cat. No. A24354) separately?

    Yes, the primary antibodies from the Human Neural Stem Cell Immunocytochemistry Kit (Cat. No. A24354) can be purchased separately. Here are the catalog numbers:

    • Nestin Monoclonal Antibody (10C2), eBioscience (Cat. No. 14984382)
    • SOX2 Polyclonal Antibody (Cat. No. 481400)
    • PAX6 Polyclonal Antibody (Cat. No. 426600)
    • SOX1 Goat anti-Human, Mouse, Rat, Polyclonal, R&D Systems; available through Fisher Scientific (Cat. No. AF3369)

    The concentrations of the antibodies in the Human Neural Stem Cell Immunocytochemistry Kit are proprietary. If you purchase the above antibodies separately, you will need to determine the best concentrations to use in your assay.

    Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center

    The Human Neural Stem Cell Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

    If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Can I use the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis?

    We do not recommend using the Human Neural Stem Cell Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Do you offer the individual components of the Human Neural Stem Cell Immunocytochemistry Kit as standalone products?

    Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.