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The RapidFinder™ Pollock ID Kit enables the simple and reliable detection of Pollock DNA in food and feed samples. The method works by detection of pollock (Gadus chalcogrammus) DNA that is present in food and feed samples by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Pollock ID Kit detects and provides sensitivity down to 0.01% of pollock (Gadus chalcogrammus) DNA. The included positive control in the kit, which comprises 0.1% Pollock DNA, can be used as a reference to set the threshold of detection of the assay.
The assay is compatible with Applied Biosystems™ QuantStudio 5™ and 7500 Fast Food Safety Real-Time PCR systems.
Sample Preparation
DNA sample preparation is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using up to 20 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.
Part of a Complete Portfolio of Solutions for Fish Species Detection
The RapidFinder™ Pollock ID Kit is just one of our many available kits for the sensitive detection of various fish species in food and feed samples. Other RapidFinder™ Fish species identification kits available include those for the detection of Basa, Gadus, Haddock, Pollock, Tuna, and Specific Tuna.