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Ion PGM™ Template IA 500 Kit - FAQs

查看更多产品信息 Ion PGM™ Template IA 500 Kit - FAQs (A24622)

9 个常见问题解答

What are the components of the Ion PGM Template IA 500 Kit (Cat. No. A24622)?

Here are the kit components:

Ion PGM Template IA Supplies 500 (Part No. A24618)
- Ion OneTouch ES Supplies: 1 bag

Ion PGM Template IA Reagents 500 (Part No. A24619)
- Ion PGM Template IA Pellets 500: 4 foil pouches [1]
- Ion PGM Template IA ISP Dilution Buffer (yellow cap): 1 mL
- Ion PGM Template IA Start Solution (purple cap): 2 x 1200 µL

Ion PGM Template IA Reactions 500 (Part No. A24620)
- Ion PGM Template IA Ion Sphere Particles (orange cap): 86 µL
- Ion PGM Template IA Primer Mix S (black cap): 48 µL
- Ion PGM Template IA Primer Mix L (blue cap): 48 µL [2]
- Ion PGM Template IA Rehydration Buffer (white cap): 2 x 1.5 mL

Ion PGM Template IA Solutions (Part No. A24621)
- Ion PGM Template IA Stop Solution: 2.8 mL
- Ion PGM Template IA Recovery Solution: 7 mL
- Ion PGM Template IA Wash Solution: 4 mL
- MyOne Beads Wash Solution (green cap): 1.04 mL
- Neutralization Solution (red cap): 40 µL
- Tween Solution: 3.5 mL

Note: [1] One Ion PGM Template IA Pellet 500 per tube per pouch.
[2] Primer Mix L is intended for amplification of long templates and is not used with the Ion ReproSeq PGS View Kit

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is the Ion PGM Template IA 500 Kit compatible with the Ion Sphere Quality Control Kit?

The Ion PGM Template IA 500 Kit is not compatible with the Ion Sphere Quality Control Kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is the Ion PGM Template IA 500 Kit compatible with libraries that utilize a truncated P1 adapter?

No, the Ion PGM Template IA 500 Kit is only compatible with libraries that have a full length P1 adapter.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Is Isothermal Amplification chemistry compatible with the Ion Chef Instrument?

No, at this time it is only compatible with the Ion One Touch ES instrument.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What version of Torrent Suite Software do I need to run with the Ion PGM Template IA 500 Kit?

Torrent Suite Software v.5.0.4 or higher is needed.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What sequencing kit is the Ion PGM Template IA 500 Kit compatible with?

The Ion PGM Template IA 500 Kit is compatible with the Ion PGM Hi-Q View Sequencing Kit. It is not compatible with the Ion PGM Hi-Q Sequencing Kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the maximum size library that is compatible with the Ion PGM Template IA 500 Kit?

The Ion PGM Template IA 500 Kit is compatible with up to 500 bp read libraries, with a total library length of 580 bp (including adapters).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

With the Ion PGM Template IA 500 Kit, how long does the Isothermal Amplification (IA) step take?

The IA step takes less than two hours, with less than 30 minutes of hands on time.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long does it take to sequence samples using the Ion PGM Template IA 500 Kit?

From sample to sequence, it takes less than 12 hours.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.