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View additional product information for SimpliAmp™ Thermal Cycler - FAQs (A24811, A35422)
26 product FAQs found
热循环仪的热盖经过设计可保证对微孔板施加合适的压力，以获得高效的PCR反应。使用PCR反应管时，热盖对反应管的压力过大可导致反应管变形。为避免出现这种情况，我们建议使用下述托盘/固定装置：
•货号4381850，用于Veriti系统的MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），和Veriti热循环仪、SimpliAmp热循环仪和Proflex PCR系统兼容。
•货号403081，MicroAmp 96孔托盘/固定装置——这一托盘适用于MicroAmp联管（或MicroAmp无盖反应管，0.2 mL），可用于7000系统、2720热循环仪和GeneAmp 9700型PCR系统。
•货号4379983，用于VeriFlex模块的MicroAmp 96孔托盘——这一托盘适用于MicroAmp联管（或MicroAmp带盖反应管，0.1 mL），可用于Veriti热循环仪、Veriti快速热循环仪、SimpliAmp热循环仪和Proflex PCR系统。
与ProFlex PCR系统类似，SimpliAmp热循环仪能够通过一个可选的USB加密装置（货号A26774）进行无线连接。
如需设定一个永久恒定温度，请在程序中选择无限 (∞) 标识。
SimpliAmp热循环仪内置创新的VeriFlex模块用于提升PCR功能。三个独立的Peltier模块（各含4个纵列）提供最大的配置多样性和灵活性，并提供两个关键优势：
PCR条件优化：每一模块均可单独设置一个特定温度，非常适合用户在PCR条件优化过程中对温度进行精确控制。
运行更多实验条件：三个Peltier模块也可用于在同一反应中设定三个不同的退火温度。
Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落（touchdown PCR）。
SimpliAmp PCR仪模块的最大变温速率为4°C/秒。
其兼容的孔板和塑料耗材与ProFlex 96孔PCR系统相同，您可点击 此处(https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html)浏览。
我们仅提供不可更换的标准96孔模块（0.2 mL）。
两种托盘适用于不同的反应管：
•适用于Veriti系统的MicroAmp 96孔托盘/固定装置（货号4381850） - 这一托盘适用于0.2mL不带盖单管（货号N8010533, N8011533），0.2 mL 带圆盖的8连管(货号A30589)和0.2 mL无盖连管(Cat 4316567) 。
•用于VeriFlex模块的MicroAmp 96孔托盘(货号4379983)-此托盘与0.1 mL带盖的单管或连管一起使用。
SimpliAmp热循环仪的存储容量在5.9GB左右，其中大部分内存空间用于保存运行方法与运行记录。本仪器能够储存120万条运行方式。
SimpliAmp PCR仪可用于维持4°C恒温条件。此外，我们的内部QC检测会要求仪器通过“无渗漏（no leak）”测试，以确保任何聚集起来的冷凝水（由于模块长时间维持在低温而产生的冷凝）不会漏入仪器中导致电力故障。
The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:
Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
Find additional tips, troubleshooting help, and resources within our PCR Thermal Cyclers Support Center.
Like the ProFlex PCR System, the SimpliAmp Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.
To add an infinite hold, select the infinity symbol for program.
The SimpliAmp Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:
- PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The maximum block ramp rate of the SimpliAmp Thermal Cycler is 4 degrees C/sec.
The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html).
We offer only a 96-well standard (0.2 mL) block that is not interchangeable.
The two different types of trays are for use with different tubes:
-MicroAmp 96-well Tray/Retainer Set for Veriti Systems (Cat. No. 4381850) - this tray is for use with 0.2 mL single tubes without caps (Cat. Nos. N8010533, N8011533), 0.2 mL 8-tube strip with attached dome caps (Cat. No. A30589), and 0.2 mL tube strips without caps (Cat. No. 4316567)
-MicroAmp 96-well Tray for VeriFlex Blocks (Cat. No. 4379983) - this tray is for use with 0.2 mL single tubes with caps (Cat. Nos. N8010540, N8011540)
The storage capacity for the SimpliAmp Thermal Cycler is approximately 5.9 GB. The run methods and run records stored use most of the memory. It can store 1.2 million run methods.
The SimpliAmp PCR System can be used with a 4 degrees C hold. Further, our in-house QC testing requires the passing of a “no leak” test make sure that any accumulated water (due to condensation when the block temperature is set to low temperature for a long period of time) will not leak into the instrument causing electronic failure.
Unfortunately, we do not sell the sample block for the SimpliAmp Thermal Cycler separately. Only the ProFlex series and the Automated series of thermal cyclers have interchangeable blocks that we sell separately.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.
As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.
Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf
Find additional tips, troubleshooting help, and resources within ourPCR Thermal Cyclers Support Center.
The Thermal Cycler Fleet Control Software allows monitoring of the following instruments from within a firewall:
SimpliAmp Thermal Cycler
ProFlex PCR System
Veriti Thermal Cycler
VeritiPro Thermal Cycler
Automated Thermal Cycler (ATC)
The Thermal Cycler Fleet Control Software is compatible with the following instruments:
- SimpliAmp Thermal Cycler
- ProFlex PCR System
- Veriti Thermal Cycler
- VeritiPro Thermal Cycler
- Automated Thermal Cycler (ATC)
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler