Shifting the paradigm: the putative mitochondrial protein ABCB6 resides in the lysosomes of cells and in the plasma membrane of erythrocytes.
AuthorsKiss K, Brozik A, Kucsma N, Toth A, Gera M, Berry L, Vallentin A, Vial H, Vidal M, Szakacs G
JournalPLoS One
PubMed ID22655043
ABCB6, a member of the adenosine triphosphate-binding cassette (ABC) transporter family, has been proposed to be responsible for the mitochondrial uptake of porphyrins. Here we show that ABCB6 is a glycoprotein present in the membrane of mature erythrocytes and in exosomes released from reticulocytes during the final steps of erythroid ... More
Molecular integrity and global gene expression of breast and lung cancer stem cells under long-term storage and recovery.
AuthorsKarimi-Busheri F, Zadorozhny V, Carrier E, Fakhrai H
JournalCell Tissue Bank
PubMed ID22592563
'Cryopreservation is a common procedure widely used in biological and clinical sciences. Similar protocols are also applied in preserving cancer stem cells, a field with high promises and challenges. Specific cell surface membrane proteins are considered to be biomarkers of cancer stem cells and they may play a critical role ... More
CD44 is a negative cell surface marker for pluripotent stem cell identification during human fibroblast reprogramming.
AuthorsQuintanilla RH, Asprer JS, Vaz C, Tanavde V, Lakshmipathy U
Journal
PubMed ID24416407
'Induced pluripotent stem cells (iPSCs) are promising tools for disease research and cell therapy. One of the critical steps in establishing iPSC lines is the early identification of fully reprogrammed colonies among unreprogrammed fibroblasts and partially reprogrammed intermediates. Currently, colony morphology and pluripotent stem cell surface markers are used to ... More
Enhanced dispersibility and cellular transmembrane capability of single-wall carbon nanotubes by polycyclic organic compounds as chaperon.
AuthorsWang L, Zhang L, Xue X, Ge G, Liang X
JournalNanoscale
PubMed ID22628008
'The common aggregation of single-wall carbon nanotube (SWCNT) in solution is the critical obstacle to elucidate their unique physico-chemical characteristics and biological properties. Therefore, it is very important to overcome this barrier through manipulation of the weak interaction of small molecules with nanotube surface limited interface. A highly dispersed SWCNT ... More
CD86 is an activation receptor for NK cell cytotoxicity against tumor cells.
AuthorsPeng Y, Luo G, Zhou J, Wang X, Hu J, Cui Y, Li XC, Tan J, Yang S, Zhan R, Yang J, He W, Wu J
Journal
PubMed ID24349559
'CTLA4Ig has been successfully used in the clinic for suppression of T cell activation. However, patients treated with CTLA4Ig experienced reduced incidence of tumors than predicted, but the underlying mechanism remains unknown. In this paper, we showed that brief administration of CTLA4Ig significantly reduced tumor metastasis and prolonged the survival ... More
Cellular vacuolation and mitochondrial-associated factors induced by Clostridium perfringens epsilon toxin detected using acoustic flow cytometry.
AuthorsFerrarezi MC, Curci VC, Cardoso TC
Journal
PubMed ID24076036
'Epsilon toxin (ETX) produced by Clostridium perfringens types B and D is a potent toxin that is responsible for fatal enterotoxaemia. In vitro, ETX, which is considered as a pore-forming toxin, forms a heptamer in Madin-Darby canine kidney (MDCK) cell membranes, which is considered to be a pre-pore stage. After binding ... More
IP3R2 levels dictate the apoptotic sensitivity of diffuse large B-cell lymphoma cells to an IP3R-derived peptide targeting the BH4 domain of Bcl-2.
AuthorsAkl H, Monaco G, La Rovere R, Welkenhuyzen K, Kiviluoto S, Vervliet T, Molgó J, Distelhorst CW, Missiaen L, Mikoshiba K, Parys JB, De Smedt H, Bultynck G
Journal
PubMed ID23681227
'Disrupting inositol 1,4,5-trisphosphate (IP3) receptor (IP3R)/B-cell lymphoma 2 (Bcl-2) complexes using a cell-permeable peptide (stabilized TAT-fused IP3R-derived peptide (TAT-IDP(S))) that selectively targets the BH4 domain of Bcl-2 but not that of B-cell lymphoma 2-extra large (Bcl-Xl) potentiated pro-apoptotic Ca(2+) signaling in chronic lymphocytic leukemia cells. However, the molecular mechanisms rendering ... More
Efficient programming of human eye conjunctiva-derived induced pluripotent stem (ECiPS) cells into definitive endoderm-like cells.
AuthorsMassumi M, Hoveizi E, Baktash P, Hooti A, Ghazizadeh L, Nadri S, Pourasgari F, Hajarizadeh A, Soleimani M, Nabiuni M, Khorramizadeh MR
Journal
PubMed ID24434355
'Due to pluripotency of induced pluripotent stem (iPS) cells, and the lack of immunological incompatibility and ethical issues, iPS cells have been considered as an invaluable cell source for future cell replacement therapy. This study was aimed first at establishment of novel iPS cells, ECiPS, which directly reprogrammed from human ... More
Synergistic effect of hydrogen peroxide on polyploidization during the megakaryocytic differentiation of K562 leukemia cells by PMA.
AuthorsOjima Y, Duncan MT, Nurhayati RW, Taya M, Miller WM
Journal
PubMed ID23770036
'The human myelogenous cell line, K562 has been extensively used as a model for the study of megakaryocytic (MK) differentiation, which could be achieved by exposure to phorbol 12-myristate 13-acetate (PMA). In this study, real-time PCR analysis revealed that the expression of catalase (cat) was significantly repressed during MK differentiation ... More
Selective targeting of the repressive transcription factors YY1 and cMyc to disrupt quiescent human immunodeficiency viruses.
AuthorsBarton K, Margolis D
JournalAIDS Res Hum Retroviruses
PubMed ID22866663
'Quiescent HIV-1 infection of resting CD4(+) T cells is an obstacle to eradication of HIV-1 infection. These reservoirs are maintained, in part, by repressive complexes that bind to the HIV-1 long terminal repeat (LTR) and recruit histone deacetylases (HDACs). cMyc and YY1 are two transcription factors that are recruited as ... More
Investigation of deregulated genes of Notch signaling pathway in human T cell acute lymphoblastic leukemia cell lines and clinical samples.
AuthorsParyan M, Mohammadi-Yeganeh S, Samiee SM, Soleimani M, Arefian E, Azadmanesh K, Poopak B, Mostafavi E, Karimipoor M, Mahdian R
Journal
PubMed ID23982259
'In diagnostic research challenges, quantitative real-time PCR (QPCR) has been widely utilized in gene expression analysis because of its sensitivity, accuracy, reproducibility, and most importantly, quantitativeness. Real-time PCR base kits are wildly applicable in cancer signaling pathways, especially in cancer investigations. T-cell acute lymphoblastic leukemia (T-ALL) is a type of ... More
Human antibody responses to pneumococcal surface protein A and capsular polysaccharides during acute and convalescent stages of invasive disease in adult patients.
AuthorsKolberg J, Aase A, Naess LM, Aaberge IS, Caugant DA
Journal
PubMed ID24151210
'The IgG antibody responses to pneumococcal surface protein A (PspA) and capsular polysaccharides in acute and convalescent-phase sera from 10 adult patients with invasive pneumococcal disease were analysed. The relatedness between the strains were characterized by capsular serotyping (1, 4, 7F, 9V, 12F and 19F), multilocus sequence typing (MLST) and ... More
Enhanced siRNA delivery and silencing gold-chitosan nanosystem with surface charge-reversal polymer assembly and good biocompatibility.
AuthorsHan L, Zhao J, Zhang X, Cao W, Hu X, Zou G, Duan X, Liang XJ,
JournalACS Nano
PubMed ID22838646
'A simple nanocarrier coated with chitosan and the pH-responsive charge-reversible polymer, PAH-Cit, was constructed using layer-by-layer assembly to deliver siRNA. Gold nanoparticles (AuNPs) were di-rectly reduced and stabilized by chitosan (CS), forming a positively charged AuNP-CS core. Charge-reversible PAH-Cit and polyethylenimine (PEI) were sequentially deposited onto the surface of AuNP-CS ... More
Coordination between TLR9 signaling in macrophages and CD3 signaling in T cells induces robust expression of IL-30.
AuthorsDibra D, Cutrera JJ, Li S,
JournalJ Immunol
PubMed ID22407920
'IL-30, the p28 subunit of IL-27, interacts with EBV-induced gene 3 to form IL-27, which modulates both proinflammatory and anti-inflammatory responses during autoimmune or infectious disease. It also acts as a natural antagonist of gp130, thereby attenuating the signals of other gp130-associated cytokines. IL-30 regulation via LPS has been reported ... More
Altered microRNA expression in B lymphocytes in multiple sclerosis: towards a better understanding of treatment effects.
AuthorsSievers C, Meira M, Hoffmann F, Fontoura P, Kappos L, Lindberg RL
JournalClin Immunol
PubMed ID22659298
'MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression. We compared the expression of 1059 miRNAs in B lymphocytes from untreated and natalizumab treated relapsing-remitting multiple sclerosis (RRMS) patients and healthy volunteers (HV). Forty nine miRNAs were down-regulated in untreated MS patients compared with HV. A distinct pattern of 10 differentially ... More
FCER2 (CD23) asthma-related single nucleotide polymorphisms yields increased IgE binding and Egr-1 expression in human B cells.
'CD23 is the low-affinity Fc receptor for IgE. When expressed on B cells, CD23 appears to play a role in regulation of IgE synthesis. Polymorphisms within FCER2, the gene encoding CD23, have been associated with atopy, increased risk of exacerbations in patients with asthma, and high serum IgE levels. A ... More
Functional innervation of hepatic iNKT cells is immunosuppressive following stroke.
AuthorsWong CH, Jenne CN, Lee WY, Léger C, Kubes P,
JournalScience
PubMed ID21921158
'Systemic immunosuppression has been associated with stroke for many years, but the underlying mechanisms are poorly understood. In this study, we demonstrated that stroke induced profound behavioral changes in hepatic invariant NKT (iNKT) cells in mice. Unexpectedly, these effects were mediated by a noradrenergic neurotransmitter rather than a CD1d ligand ... More
High-throughput monitoring of plant nuclear DNA contents via flow cytometry.
AuthorsGalbraith DW, Lambert GM
JournalMethods Mol Biol
PubMed ID22893296
'Interest in measuring the nuclear holoploid genome sizes of higher plants reflects not just the status of the nucleus as a defining characteristic of eukaryotic organisms. Higher plants also attract interest in that they display an unusually large range of genome sizes, current measurements indicating an almost 2,500-fold difference between ... More
Association of p62/SQSTM1 excess and oral carcinogenesis.
AuthorsInui T, Chano T, Takikita-Suzuki M, Nishikawa M, Yamamoto G, Okabe H
Journal
PubMed ID24086340
'p62/SQSTM1 (sequestosome1) has never been evaluated in oral epithelium. In order to clarify the role of p62/SQSTM1 in carcinogenesis in oral epithelium, both p62/SQSTM1 and Nrf2 were immunohistochemically evaluated in 54 carcinomas and 14 low grade dysplasias. p62/SQSTM1 knockdowns were also designed in oral cancer cells, and we analyzed the ... More
A universal gene carrier platform for treatment of human prostatic carcinoma by p53 transfection.
AuthorsHan L, Zhao J, Liu J, Duan XL, Li LH, Wei XF, Wei Y, Liang XJ
Journal
PubMed ID24411335
'Our previous work showed that a charge-reversal layer-by-layer nanosystem, PEI/PAH-Cit/AuNP-CS, effectively facilitates cellular uptake of siRNA and enhances the silencing efficacy of MDR1 siRNA. Here, the plasmid loading capacity of this vehicle was examined using EGFP-N1, and the plasmid release profile was determined in response to pH changes. The cytotoxicity ... More
ON 01910.Na is selectively cytotoxic for chronic lymphocytic leukemia cells through a dual mechanism of action involving PI3K/AKT inhibition and induction of oxidative stress.
AuthorsChapman CM, Sun X, Roschewski M, Aue G, Farooqui M, Stennett L, Gibellini F, Arthur D, Pérez-Galán P, Wiestner A
JournalClin Cancer Res
PubMed ID22351695
'Chronic lymphocytic leukemia (CLL), a malignancy of mature B cells, is incurable with chemotherapy. Signals from the microenvironment support leukemic cell survival and proliferation and may confer chemotherapy resistance. ON 01910.Na (Rigosertib), a multikinase phosphoinositide 3-kinase (PI3K) inhibitor, is entering phase III trials for myelodysplastic syndrome. Our aim was to ... More
Regression of atherosclerosis with anti-CD3 antibody via augmenting a regulatory T-cell response in mice.
AuthorsKita T, Yamashita T, Sasaki N, Kasahara K, Sasaki Y, Yodoi K, Takeda M, Nakajima K, Hirata K
Journal
PubMed ID24403315
'Although recent animal studies have investigated the cellular and molecular mechanisms underlying the process of atherosclerosis regression, it remains unknown whether adaptive immune responses including T cells are involved in this process. We investigated the role of T cells in atherosclerosis regression.' ... More
Caffeoylserotonin suppresses THP-1 monocyte adhesion and migration via inhibition of the integrin ß1/FAK/Akt signalling pathway.
AuthorsYoon JH, Kim HE, Choi JY, Bae HJ, Lee SG
JournalFitoterapia
PubMed ID22579840
'We investigated the effect of caffeoylserotonin (CaS) on THP-1 monocyte migration and adhesion to fibronectin in response to MCP-1. CaS decreased monocyte adhesion and migration induced by MCP-1, together with CCR2 expression and a5ß1 integrin, and activated ß1 integrin expression on the cell surface. CaS also inhibited FAK and Akt ... More
Non-myeloablative conditioning is sufficient to induce mixed chimerism and subsequent acceptance of donor specific cardiac and skin grafts.
AuthorsLiu C, Zhu P, Saito T, Isaka Y, Nagahara Y, Zhuang J, Li XK
JournalInt Immunopharmacol
PubMed ID23428909
'Organ transplant recipients have elevated cancer and viral infection risks due to immunosuppression and long-term results of organ transplantation remain unsatisfactory, mainly because of chronic rejection. The purpose of the current study is to establish a nonmyeloablative perioperative regimen, able to induce mixed chimerism and tolerance of allografts. To establish ... More
Cytotoxic effect of Amphotericin B in a myofibroblast cell line.
AuthorsUribe CC, Dos Santos de Oliveira F, Grossmann B, Kretzmann NA, Reverbel da Silveira T, Giugliani R, Matte U
Journal
PubMed ID23988732
'In this study we investigate whether Amphotericin B (AmB), a widely used antifungal agent, could decrease the proliferation of a myofibroblast cell line - GRX, a model of activated hepatic stellate cells (HSC). Three different hepatic cell lines (GRX, Hep G2 and ARL-6) were treated with two concentrations of AmB ... More
Lack of correlation between the amplitudes of TRP channel-mediated responses to weak and strong stimuli in intracellular Ca(2+) imaging experiments.
AuthorsAlpizar YA, Sanchez A, Radwan A, Radwan I, Voets T, Talavera K
Journal
PubMed ID24079971
'It is often observed in intracellular Ca(2+) imaging experiments that the amplitudes of the Ca(2+) signals elicited by newly characterized TRP agonists do not correlate with the amplitudes of the responses evoked subsequently by a specific potent agonist. We investigated this rather controversial phenomenon by first testing whether it is ... More
Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
AuthorsGarcía-Castro B, Alvarez-Zavala M, Riveros-Magaña AR, Ortíz-Lazareno PC, Ratkovich-González S, Hernández-Flores G, Bravo-Cuellar A, Jave-Suarez LF, Aguilar-Lemarroy A
Journal
PubMed ID24274766
'WNT signaling pathways are significantly altered during cancer development. Vertebrates possess two classes of WNT signaling pathways: the "canonical" WNT/ß-catenin signaling pathway, and the "non-canonical" pathways including WNT/Ca²? and WNT/Planar cell polarity [PCP] signaling. WNT4 influences hematopoietic progenitor cell expansion and survival; however, WNT4 function in cancer development and the ... More
Sivelestat sodium hydrate inhibits neutrophil migration to the vessel wall and suppresses hepatic ischemia-reperfusion injury.
AuthorsSakai S, Tajima H, Miyashita T, Nakanuma S, Makino I, Hayashi H, Nakagawara H, Kitagawa H, Fushida S, Fujimura T, Saito H, Munesue S, Yamamoto Y, Ohta T
Journal
PubMed ID24318803
'Sivelestat sodium hydrate (sivelestat) is a specific neutrophil elastase inhibitor that is effective in treating acute lung injury associated with systemic inflammatory response syndrome. As such, it may be useful in treating hepatic ischemia-reperfusion injury (IRI), a condition in which neutrophils transmigrate into the interstitium, leading to release of neutrophil ... More
Susceptibility of neuron-like cells derived from bovine Wharton's jelly to bovine herpesvirus type 5 infections.
AuthorsCardoso TC, Novais JB, Antello TF, Silva-Frade C, Ferrarezi MC, Ferrari HF, Gameiro R, Flores EF
JournalBMC Vet Res
PubMed ID23227933
'Bovine herpesvirus type 5 (BoHV-5), frequently lethal in cattle, is associated with significant agricultural economic losses due to neurological disease. Cattle and rabbits are frequently used as models to study the biology and pathogenesis of BoHV-5 infection. In particular, neural invasion and proliferation are two of the factors important in ... More
Design, synthesis, and biological evaluation of the analogues of glaziovianin A, a potent antitumor isoflavone.
AuthorsHayakawa I, Ikedo A, Chinen T, Usui T, Kigoshi H,
JournalBioorg Med Chem
PubMed ID22921744
'Various analogues of glaziovianin A, an antitumor isoflavone, were synthesized, and their biological activities were evaluated. O(7)-modified glaziovianin A showed strong cytotoxicity against HeLa S(3) cells. Compared to glaziovianin A, the O(7)-benzyl and O(7)-propargyl analogues were more cytotoxic against HeLa S(3) cells and more potent M-phase inhibitors. Furthermore, O(7)-modified molecular ... More
Imaging Intracellular Anticancer Drug Delivery by Self-Assembly Micelles with Aggregation-Induced Emission (AIE Micelles).
AuthorsZhang C, Jin S, Li S, Xue X, Liu J, Huang Y, Jiang Y, Chen WQ, Zou G, Liang XJ
Journal
PubMed ID24606837
'Nanoformulations show many therapeutic advantages over conventional formulations. We seek to develop traceable nanoformulations in order to closely monitor delivery. Herein, we developed a new drug delivery system (DDS) using tetraphenylethene (TPE) to fabricate a self-assembly micelle with aggregation-induced emission (AIE micelle). AIE makes the nanocarriers visible for high-quality imaging, ... More
HA14-1, but not the BH3 mimetic ABT-737, causes Ca2+ dysregulation in platelets and human cell lines.
AuthorsAkl H, Vandecaetsbeek I, Monaco G, Kauskot A, Luyten T, Welkenhuyzen K, Hoylaerts M, De Smedt H, Parys JB, Bultynck G,
JournalHaematologica
PubMed ID23403318
No Abstract
The cell-to-cell coordination between activated T cells and CpG-stimulated macrophages synergistically induce elevated levels of IL-10 via NF-?B1, STAT3, and CD40/CD154.
AuthorsDibra D, Li S
Journal
PubMed ID24330710
Studies into the regulation of interleukin-10 (IL-10), have focused only on the molecular or single-cell level. The cues that induce IL-10 in the context of cell-to-cell communication are scarce. To fill this gap, this study elucidates the cell-to-cell interaction dependent regulation of IL-10. ... More
Decrease in hyperosmotic stress-induced corneal epithelial cell apoptosis by L-carnitine.
To characterize the osmoprotective properties of L-carnitine on human corneal epithelial cell volume and apoptosis during hyperosmotic stress.
Sorafenib inhibits cell migration and stroma-mediated bortezomib resistance by interfering B-cell receptor signaling and protein translation in mantle cell lymphoma.
AuthorsXargay-Torrent S, López-Guerra M, Montraveta A, Saborit-Villarroya I, Rosich L, Navarro A, Pérez-Galán P, Roué G, Campo E, Colomer D
JournalClin Cancer Res
PubMed ID23231952
We evaluated the antitumoral properties of the multikinase inhibitor sorafenib in mantle cell lymphoma (MCL), an aggressive B lymphoma for which current therapies have shown limited efficacy. ... More
CO2 gas induced drug release from pH-sensitive liposome to circumvent doxorubicin resistant cells.
AuthorsLiu J, Ma H, Wei T, Liang XJ,
JournalChem Commun (Camb)
PubMed ID22498879
A novel pH-sensitive liposome encapsulating doxorubicin was prepared by a NH(4)HCO(3) gradient method. The liposomes were able to release the drug at pH 5.0 by the production of CO(2) gas. More importantly, the drug-loaded liposome effectively circumvented the breast cancer cells resistant to doxorubicin. ... More
Biological characterization of the antiproliferative potential of Co(II) and Sn(IV) coordination compounds in human cancer cell lines: a comparative proteomic approach.
AuthorsSilva A, Luís D, Santos S, Silva J, Mendo AS, Coito L, Silva TF, da Silva MF, Martins LM, Pombeiro AJ, Borralho PM, Rodrigues CM, Cabral MG, Videira PA, Monteiro C, Fernandes AR
Journal
PubMed ID23800656
The discovery of cisplatin's antitumor activity led to a great interest in the potential application of coordination compounds as chemotherapeutic agents. It is essential to identify new compounds that selectively inhibit tumor proliferation, evading secondary effects and resistance associated with chemotherapeutics. ... More
CD24+/CD38- as new prognostic marker for non-small cell lung cancer.
AuthorsKarimi-Busheri F, Rasouli-Nia A, Zadorozhny V, Fakhrai H
Journal
PubMed ID24094028
Lung cancer is the leading cause of death among cancers in the world. The annual death toll due to this disease exceeds the combined deaths caused by colon, breast, prostate, and pancreatic cancers. As a result, there has been a tremendous effort to identify new biomarkers for early detection and ... More
CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors.
AuthorsHu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S
Journal
PubMed ID24565056
Increased infiltration of CD8+T cells into tumors has a positive impact on survival. Our previous study showed that doxorubicin (Dox) plus interleukin-12 (IL-12) boosted the accumulation of CD8+T cells in tumors and had a greater antitumor effect than did either agent alone. The purpose of this study was to determine ... More
Fibroblasts expressing the thyrotropin receptor overarch thyroid and orbit in Graves' disease.
AuthorsSmith TJ, Padovani-Claudio DA, Lu Y, Raychaudhuri N, Fernando R, Atkins S, Gillespie EF, Gianoukakis AG, Miller BS, Gauger PG, Doherty GM, Douglas RS
JournalJ Clin Endocrinol Metab
PubMed ID21956421
Graves' disease (GD) is a systemic autoimmune syndrome comprising manifestations in thyroid and orbital connective tissue. The link between these two tissues in GD eludes our understanding. Patients with GD have increased frequency of circulating monocyte lineage cells known as fibrocytes. These fibrocytes infiltrate orbital connective tissues in thyroid-associated ophthalmopathy ... More
Long-Acting Atypical Antipsychotics: Characterization of the Local Tissue Response.
AuthorsPaquette SM, Dawit H, Hickey MB, Merisko-Liversidge E, Almarsson O, Deaver DR
Journal
PubMed ID24558010
Long-acting injectables (LAIs) are increasingly recognized as an effective therapeutic approach for treating chronic conditions. Many LAIs are formulated to create a poorly soluble depot from which the active agent is delivered over time. This long residing depot can cause localized chronic-active inflammation in the tissue, which has not been ... More
MicroRNAs (miRNAs) have emerged as a family of post-transcriptional regulators of gene expression that mediate diverse aspects of immunity. MiRNA dysregulation has been found in multiple sclerosis (MS), reflecting the growing need to identify disease-specific miRNA expression signatures. Our previous low-density array studies reveal differential miR-126 expression in the CD4(+)T ... More
Flow cytometry: retrospective, fundamentals and recent instrumentation.
AuthorsPicot J, Guerin CL, Le Van Kim C, Boulanger CM,
JournalCytotechnology
PubMed ID22271369
Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of ... More
Large-scale cell production of stem cells for clinical application using the automated cell processing machine.
AuthorsKami D, Watakabe K, Yamazaki-Inoue M, Minami K, Kitani T, Itakura Y, Toyoda M, Sakurai T, Umezawa A, Gojo S
Journal
PubMed ID24228851
Cell-based regeneration therapies have great potential for application in new areas in clinical medicine, although some obstacles still remain to be overcome for a wide range of clinical applications. One major impediment is the difficulty in large-scale production of cells of interest with reproducibility. Current protocols of cell therapy require ... More
Cellular characterization of human pluripotent stem cells.
AuthorsQuintanilla RH,
JournalMethods Mol Biol
PubMed ID23546756
Human pluripotent stem cells (PSCs), in particular induced PSCs, are very difficult to derive, grow, and bank. They require extensive amounts of resources and time to render them useful for basic and applied research. As the derivation methods, culture systems and tissues of origin differ, so does the quality of ... More
SOX11 regulates PAX5 expression and blocks terminal B-cell differentiation in aggressive mantle cell lymphoma.
AuthorsVegliante MC, Palomero J, Pérez-Galán P, Roué G, Castellano G, Navarro A, Clot G, Moros A, Suárez-Cisneros H, Beà S, Hernández L, Enjuanes A, Jares P, Villamor N, Colomer D, Martín-Subero JI, Campo E, Amador V,
JournalBlood
PubMed ID23321250
Mantle cell lymphoma (MCL) is one of the most aggressive lymphoid neoplasms whose pathogenesis is not fully understood. The neural transcription factor SOX11 is overexpressed in most MCL but is not detected in other mature B-cell lymphomas or normal lymphoid cells. The specific expression of SOX11 in MCL suggests that ... More
Live cell cycle analysis of Drosophila tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle violet DNA stain.
AuthorsFlegel K, Sun D, Grushko O, Ma Y, Buttitta L,
Journal
PubMed ID23712023
Flow cytometry has been widely used to obtain information about DNA content in a population of cells, to infer relative percentages in different cell cycle phases. This technique has been successfully extended to the mitotic tissues of the model organism Drosophila melanogaster for genetic studies of cell cycle regulation in ... More
Antitumoral Activity of Lenalidomide in In Vitro and In Vivo Models of Mantle Cell Lymphoma Involves the Destabilization of Cyclin D1/p27KIP1 Complexes.
AuthorsMoros A, Bustany S, Cahu J, Saborit-Villarroya I, Martínez A, Colomer D, Sola B, Roué G,
Journal
PubMed ID24178620
Clinical responses to the immmunomodulatory drug lenalidomide have been observed in patients with relapsed/refractory mantle cell lymphoma (MCL), although its mechanism of action remains partially unknown. We investigated whether the expression and subcellular localization of cyclin D1, a major cell-cycle regulator overexpressed in MCL, and the cyclin-dependent kinase inhibitor p27(KIP1), ... More
Size-dependent localization and penetration of ultrasmall gold nanoparticles in cancer cells, multicellular spheroids, and tumors in vivo.
AuthorsHuang K, Ma H, Liu J, Huo S, Kumar A, Wei T, Zhang X, Jin S, Gan Y, Wang PC, He S, Zhang X, Liang XJ,
JournalACS Nano
PubMed ID22540892
This work demonstrated that ultrasmall gold nanoparticles (AuNPs) smaller than 10 nm display unique advantages over nanoparticles larger than 10 nm in terms of localization to, and penetration of, breast cancer cells, multicellular tumor spheroids, and tumors in mice. Au@tiopronin nanoparticles that have tunable sizes from 2 to 15 nm ... More
NIST/ISAC standardization study: variability in assignment of intensity values to fluorescence standard beads and in cross calibration of standard beads to hard dyed beads.
AuthorsHoffman RA, Wang L, Bigos M, Nolan JP,
JournalCytometry A
PubMed ID22915363
Results from a standardization study cosponsored by the International Society for Advancement of Cytometry (ISAC) and the US National Institute of Standards and Technology (NIST) are reported. The study evaluated the variability of assigning intensity values to fluorophore standard beads by bead manufacturers and the variability of cross calibrating the ... More
The involvement of CD36 in monocyte activation by antiphospholipid antibodies.
AuthorsKato M, Atsumi T, Oku K, Amengual O, Nakagawa H, Fujieda Y, Otomo K, Horita T, Yasuda S, Koike T,
Journal
PubMed ID23817509
CD36, known as a scavenger receptor, is a transmembrane glycoprotein expressed on monocytes, platelets and endothelial cells, recognizes multiple ligands, including phosphatidylserine, and regulates atherogenesis and thrombosis. The objective of this study is to investigate the possible involvement of CD36 in the pathophysiology of thrombosis in patients with antiphospholipid syndrome ... More
Electrophysiological integration and action potential properties of transplanted cardiomyocytes derived from induced pluripotent stem cells.
AuthorsHalbach M, Peinkofer G, Baumgartner S, Maass M, Wiedey M, Neef K, Krausgrill B, Ladage D, Fatima A, Saric T, Hescheler J, Müller-Ehmsen J,
Journal
PubMed ID24042016
Induced pluripotent stem cell-derived cardiomyocytes (iPSCM) are regarded as promising cell type for cardiac cell replacement therapy. We investigated long-term electrophysiological integration and maturation of transplanted iPSCM, which are essential for therapeutic benefit. Murine iPSCM expressing enhanced green fluorescent protein and a puromycin resistance under control of the a-myosin heavy ... More
Evaluation and mechanistic analysis of the cytotoxicity of the acyl glucuronide of nonsteroidal anti-inflammatory drugs.
AuthorsMiyashita T, Kimura K, Fukami T, Nakajima M, Yokoi T,
Journal
PubMed ID24104198
The chemical reactivity of acyl glucuronide (AG) has been thought to be associated with the toxic properties of drugs containing carboxylic acid moieties, but there has been no direct evidence showing that AG formation is related to the observed toxicity. In the present study, the cytotoxicity of AGs, especially that ... More
Dual CDK4/CDK6 Inhibition Induces Cell-Cycle Arrest and Senescence in Neuroblastoma.
AuthorsRader J, Russell MR, Hart LS, Nakazawa MS, Belcastro LT, Martinez D, Li Y, Carpenter EL, Attiyeh EF, Diskin SJ, Kim S, Parasuraman S, Caponigro G, Schnepp RW, Wood AC, Pawel B, Cole KA, Maris JM,
Journal
PubMed ID24045179
Neuroblastoma is a pediatric cancer that continues to exact significant morbidity and mortality. Recently, a number of cell-cycle proteins, particularly those within the Cyclin D/CDK4/CDK6/RB network, have been shown to exert oncogenic roles in neuroblastoma, suggesting that their therapeutic exploitation might improve patient outcomes. Experimental Procedures: We evaluated the effect ... More
Single-cell adhesion tests against functionalized microspheres arrayed on AFM cantilevers confirm heterophilic E- and N-cadherin binding.
AuthorsOunkomol C, Yamada S, Heinrich V,
JournalBiophys J
PubMed ID21156120
We assess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force microscope with micropipette manipulation. It enables ... More
Flow cytometry of the side population (SP).
AuthorsPetriz J,
JournalCurr Protoc Cytom
PubMed ID23546779
The side population (SP) has become an important hallmark for the definition of the stem-cell compartment, especially for the detection of stem cells and for their physical isolation by fluorescence-activated cell sorting (FACS). SP cells are CD34(-) and were discovered using ultraviolet excitation based on the efflux of Hoechst 33342 ... More
Universal marker and detection tool for human sarcoma circulating tumor cells.
AuthorsSatelli A, Mitra A, Cutrera JJ, Devarie M, Xia X, Ingram DR, Dibra D, Somaiah N, Torres KE, Ravi V, Ludwig JA, Kleinerman ES, Li S
Journal
PubMed ID24448245
To date, no specific marker exists for the detection of circulating tumor cells (CTC) from different types of sarcomas, though tools are available for detection of CTCs in peripheral blood of patients with cancer for epithelial cancers. Here, we report cell-surface vimentin (CSV) as an exclusive marker on sarcoma CTC ... More
Isolation and characterization of Wharton's jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system.
AuthorsCardoso TC, Ferrari HF, Garcia AF, Novais JB, Silva-Frade C, Ferrarezi MC, Andrade AL, Gameiro R
JournalBMC Biotechnol
PubMed ID22559872
The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about ... More
Generation of tumor-targeted antibody-CpG conjugates.
AuthorsLi Z, Jang JK, Lechner MG, Hu P, Khawli L, Scannell CA, Epstein AL
JournalJ Immunol Methods
PubMed ID23279945
A number of monoclonal antibodies against tumor-associated antigens have been developed for the treatment of cancer. The anti-tumor effects of such antibodies can be enhanced by conjugation to immune stimulatory ligands, such as the toll-like receptor 9 agonist CpG oligodeoxynucleotides (CpG). The present study describes methods for the conjugation of ... More
Multifunctional hybrid silica nanoparticles for controlled doxorubicin loading and release with thermal and pH dually response.
AuthorsHu X, Hao X, Wu Y, Zhang J, Zhang X, Wang PC, Zou G, Liang XJ
JournalJ Mater Chem B Mater Biol Med
PubMed ID23543911
Controlled drug loading and release into tumor cells to increase the intracellular drug concentration is a major challenge for cancer therapy due to resistance and inefficient cellular uptake. Here a temperature and pH dually responsive PNiPAM/AA@SiO2 core-shell particles with internal controlled release were designed and fabricated for efficient cancer treatment, ... More
Responses to telomere erosion in plants.
AuthorsAmiard S, Da Ines O, Gallego ME, White CI
Journal
PubMed ID24465970
In striking contrast to animals, plants are able to develop and reproduce in the presence of significant levels of genome damage. This is seen clearly in both the viability of plants carrying knockouts for key recombination and DNA repair genes, which are lethal in vertebrates, and in the impact of ... More
ß-Catenin-dependent pathway activation by both promiscuous "canonical" WNT3a-, and specific "noncanonical" WNT4- and WNT5a-FZD receptor combinations with strong differences in LRP5 and LRP6 dependency.
AuthorsRing L, Neth P, Weber C, Steffens S, Faussner A
Journal
PubMed ID24269653
The WNT/ß-catenin signalling cascade is the best-investigated frizzled receptor (FZD) pathway, however, whether and how specific combinations of WNT/FZD and co-receptors LRP5 and LRP6 differentially affect this pathway are not well understood. This is mostly due to the fact that there are 19 WNTs, 10 FZDs and at least two ... More
Hyaluronic acid-chitosan nanoparticles for co-delivery of MiR-34a and doxorubicin in therapy against triple negative breast cancer.
AuthorsDeng X, Cao M, Zhang J, Hu K, Yin Z, Zhou Z, Xiao X, Yang Y, Sheng W, Wu Y, Zeng Y
Journal
PubMed ID24565525
Metastatic relapse, development of drug resistance in cancer cells and adverse side effects of chemotherapeutic agents are the major obstacles for effective chemotherapy against triple-negative breast cancer. To address these problems, miR-34a, a potent endogenous tumor suppressive molecule in breast cancer, was co-encapsulated with doxorubicin (DOX) into hyaluronic acid (HA)-chitosan ... More
Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model.
AuthorsBonamin LV, Sato C, Zalla Neto R, Morante G, Cardoso TN, de Santana FR, Coelho Cde P, Osugui L, Popi AF, Hurtado EC, Mariano M
JournalEvid Based Complement Alternat Med
PubMed ID23431344
The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated. The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node ... More
Inhibitory Activities of Trichostatin A in U87 Glioblastoma Cells and Tumorsphere-Derived Cells.
AuthorsSassi FD, Caesar L, Jaeger M, Nör C, Abujamra AL, Schwartsmann G, de Farias CB, Brunetto AL, Lopez PL, Roesler R
Journal
PubMed ID24464841
Epigenetic alterations have been increasingly implicated in glioblastoma (GBM) pathogenesis, and epigenetic modulators including histone deacetylase inhibitors (HDACis) have been investigated as candidate therapies. GBMs are proposed to contain a subpopulation of glioblastoma stem cells (GSCs) that sustain tumor progression and therapeutic resistance and can form tumorspheres in culture. Here, ... More
The use of spinning-disk confocal microscopy for the intravital analysis of platelet dynamics in response to systemic and local inflammation.
AuthorsJenne CN, Wong CH, Petri B, Kubes P
JournalPLoS One
PubMed ID21949865
Platelets are central players in inflammation and are an important component of the innate immune response. The ability to visualize platelets within the live host is essential to understanding their role in these processes. Past approaches have involved adoptive transfer of labelled platelets, non-specific dyes, or the use of fluorescent ... More
Endoplasmic reticulum stress plays a pivotal role in cell death mediated by the pan-deacetylase inhibitor panobinostat in human hepatocellular cancer cells.
AuthorsMontalbano R, Waldegger P, Quint K, Jabari S, Neureiter D, Illig R, Ocker M, Di Fazio P
JournalTransl Oncol
PubMed ID23544167
Panobinostat, a pan-deacetylase inhibitor, represents a novel therapeutic option for cancer diseases. Besides its ability to block histone deacetylases (HDACs) by promoting histone hyperacetylation, panobinostat interferes with several cell death pathways providing a potential efficacy against tumors. We have previously demonstrated that panobinostat has a potent apoptotic activity in vitro ... More
The anti-inflammatory effect of alloferon on UVB-induced skin inflammation through the down-regulation of pro-inflammatory cytokines.
AuthorsKim Y, Lee SK, Bae S, Kim H, Park Y, Chu NK, Kim SG, Kim HR, Hwang YI, Kang JS, Lee WJ
JournalImmunol Lett
PubMed ID23018032
UVB irradiation can induce biological changes in the skin, modulate immune responses and activate inflammatory reactions leading to skin damage. Alloferon, which is isolated from the blood of an experimentally infected insect, the blow fly Calliphora vicina, is known for its anti-viral and anti-tumor activities in mice model. However, the ... More
The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function.
AuthorsTakehara Y, Yabuuchi A, Ezoe K, Kuroda T, Yamadera R, Sano C, Murata N, Aida T, Nakama K, Aono F, Aoyama N, Kato K, Kato O
JournalLab Invest
PubMed ID23212100
The clinical application of human adipose-derived mesenchymal stem cells (MSCs) as treatment for intractable diseases or traumatic tissue damage has attracted attention. To address the ability of reactivating injured ovaries, we prepared a rat model with damaged ovaries by using an anticancer agent, cyclophosphamide (CTX). We then investigated the restorative ... More
Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity.
AuthorsSakai S, Inamoto K, Liu Y, Tanaka S, Arii S, Taya M
JournalCancer Sci
PubMed ID22168771
Multicellular tumor spheroids (MTS) are gaining increased recognition as valuable tools and key elements in anticancer drug discovery and tumor therapy test programs. However, the lack of reproducible and uniform MTS sizes is a major problem for pharmaceutical assays. Here, we show the usefulness of duplex microcapsules with a Ca-alginate ... More
Whole transcriptome analysis of the ERa synthetic fragment P295-T311 (ERa17p) identifies specific ERa-isoform (ERa, ERa36)-dependent and -independent actions in breast cancer cells.
AuthorsNotas G, Kampa M, Pelekanou V, Troullinaki M, Jacquot Y, Leclercq G, Castanas E
JournalMol Oncol
PubMed ID23474223
ERa17p is a peptide corresponding to the sequence P295LMIKRSKKNSLALSLT311 of the estrogen receptor alpha (ERa) and initially found to interfere with ERa-related calmodulin binding. ERa17p was subsequently found to elicit estrogenic responses in E2-deprived ERa-positive breast cancer cells, increasing proliferation and ERE-dependent gene transcription. Surprisingly, in E2-supplemented media, ERa17p-induced apoptosis ... More
Could a B-1 cell derived phagocyte "be one" of the peritoneal macrophages during LPS-driven inflammation?
AuthorsPopi AF, Osugui L, Perez KR, Longo-Maugéri IM, Mariano M
JournalPLoS One
PubMed ID22479646
The inflammatory response is driven by signals that recruit and elicit immune cells to areas of tissue damage or infection. The concept of a mononuclear phagocyte system postulates that monocytes circulating in the bloodstream are recruited to inflamed tissues where they give rise to macrophages. A recent publication demonstrated that ... More
Reduced PMA enhances the responsiveness of transfected THP-1 macrophages to polarizing stimuli.
AuthorsMaeß MB, Wittig B, Cignarella A, Lorkowski S
Journal
PubMed ID24269601
Macrophages are versatile cells of the immune system which react to various external stimuli through different polarization patterns which adjust the cells to the required function whether it is removal of pathogens or necrotic cells, tissue repair or propagation of inflammation. As much of macrophage behavior is determined by their ... More
Differential effects of serum heat treatment on chemotaxis and phagocytosis by human neutrophils.
AuthorsMankovich AR, Lee CY, Heinrich V
JournalPLoS One
PubMed ID23349959
Neutrophils, in cooperation with serum, are vital gatekeepers of a host's microbiome and frontline defenders against invading microbes. Yet because human neutrophils are not amenable to many biological techniques, the mechanisms governing their immunological functions remain poorly understood. We here combine state-of-the-art single-cell experiments with flow cytometry to examine how ... More
Mesothelin virus-like particle immunization controls pancreatic cancer growth through CD8+ T cell induction and reduction in the frequency of CD4+ foxp3+ ICOS- regulatory T cells.
AuthorsZhang S, Yong LK, Li D, Cubas R, Chen C, Yao Q
Journal
PubMed ID23874581
Our previous study has shown that mesothelin (MSLN) is a potential immunotherapeutic target for pancreatic cancer. Here, we further studied the immunogenicity of chimeric murine MSLN-virus-like particles (mMSLN-VLPs), their ability to break tolerance to mMSLN, a self-antigen, and deciphered the mechanism of immune responses elicited by mMSLN-VLP immunization using a ... More
In vitro and in vivo evaluation of cysteine and site specific conjugated herceptin antibody-drug conjugates.
AuthorsJackson D, Atkinson J, Guevara CI, Zhang C, Kery V, Moon SJ, Virata C, Yang P, Lowe C, Pinkstaff J, Cho H, Knudsen N, Manibusan A, Tian F, Sun Y, Lu Y, Sellers A, Jia XC, Joseph I, Anand B, Morrison K, Pereira DS, Stover D
Journal
PubMed ID24454709
Antibody drug conjugates (ADCs) are monoclonal antibodies designed to deliver a cytotoxic drug selectively to antigen expressing cells. Several components of an ADC including the selection of the antibody, the linker, the cytotoxic drug payload and the site of attachment used to attach the drug to the antibody are critical ... More
Brief Exercise Increases Peripheral Blood NK Cell Counts without Immediate Functional Changes, but Impairs their Responses to ex vivo Stimulation.
Physical as well as psychological stress increases the number of circulating peripheral blood NK cells. Whereas some studies found a positive correlation between exercise and NK cell counts and cytotoxic activity, others showed that, for example, heavy training leads to a decrease in per cell NK cytotoxicity. Thus, the impact ... More