小鼠 (ICR) 灭活胚胎成纤维细胞

小鼠胚胎成纤维细胞 (MEF) 被用作培养小鼠和人胚胎干细胞 (ESC) 及诱导性多能干细胞 (iPSC) 时的饲养层。它们是从小鼠（品系：ICR）胚胎中分离得到的了解更多信息

货号	数量
A24903	1 mL

货号 A24903

价格（CNY)

小鼠胚胎成纤维细胞 (MEF) 被用作培养小鼠和人胚胎干细胞 (ESC) 及诱导性多能干细胞 (iPSC) 时的饲养层。它们是从小鼠（品系：ICR）胚胎中分离得到的。细胞经扩增和 γ 射线辐照灭活，并冷冻保存。在冷冻保存前进行了细胞存活率测试（解冻后存活率 >80%），并通过接种低密度的灭活细胞并监测培养 14 天后细胞生长的中心确认了生长抑制情况，还测试了它们支持小鼠胚胎干细胞 (mESC)、人胚胎干细胞 (hESC) 生长的能力以及标记物表达（10 月 4 日）。

仅供科研使用。不可用于诊断程序。

供体混合供体

产品类型小鼠胚胎成纤维细胞

数量1 mL

细胞类型饲养层细胞、成纤维细胞

培养类型饲养层依赖性

Unit SizeEach

内容与储存

储存于液氮中。

常见问题解答 (FAQ)

What is the difference between Mouse (ICR) Inactivated Embryonic Fibroblasts (Cat. No. A24903) and CF1 Mouse Embryonic Fibroblasts, irradiated (Cat. Nos. A34180, A34181)? Can they be used to reprogram fibroblasts?

Mouse (ICR) Inactivated Embryonic Fibroblasts and CF1 Mouse Embryonic Fibroblasts, irradiated are inactivated feeder layer fibroblast cells that are isolated from different mouse strains. Researchers often have a preference for one or the other. Neither of these can be used to reprogram fibroblasts.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does the culture vessel need to be coated prior to seeding Mouse (ICR) Inactivated Fibroblasts?

Yes, the cutlure vessel needs to be coated with Attachment Factor Protein (Cat. No. S006100) at 37 degrees C for 30 min or at room temperature for 2 hours. The coated vessels can be used immediately or stored at room temperature for up to 24 hours.

What is the sex of mouse embryros from which Mouse (ICR) Inactivated Fibroblasts are isolated?

We do not determine the sex of the embryos. The embryos were pooled from several litters which are a mixture of male and female.