Correlative microscopy of ultrathin cryosections is a powerful tool for placental research.
AuthorsTakizawa T, Robinson JM
JournalPlacenta
PubMed ID12744933
'In this report, we describe procedures for correlative fluorescence and electron microscopy in immunocytochemical studies on the human placenta. Ultrathin cryosections of placenta were used for detection of the distribution of antigens by immunofluorescence and subsequently by immunoelectron microscopy of the same ultrathin cryosection. This methodology has certain advantages over ... More
Specific biarsenical labeling of cell surface proteins allows fluorescent- and biotin-tagging of amyloid precursor protein and prion proteins.
AuthorsTaguchi Y, Shi ZD, Ruddy B, Dorward DW, Greene L, Baron GS,
JournalMol Biol Cell
PubMed ID18987338
Fluorescent tagging is a powerful tool for imaging proteins in living cells. However, the steric effects imposed by fluorescent tags impair the behavior of many proteins. Here, we report a novel technique, Instant with DTT, EDT, And Low temperature (IDEAL)-labeling, for rapid and specific FlAsH-labeling of tetracysteine-tagged cell surface proteins ... More
Ultrathin cryosections: an important tool for immunofluorescence and correlative microscopy.
AuthorsTakizawa T, Robinson JM
JournalJ Histochem Cytochem
PubMed ID12754282
Here we show that ultrathin cryosections of placental tissue can be used as a substrate in immunofluorescence experiments. A high degree of spatial resolution can be achieved in these preparations because there is essentially no out-of-focus fluorescence. Therefore, immunofluorescence microscopy using ultrathin cryosections provides a very useful method for determining ... More