CD44 is a negative cell surface marker for pluripotent stem cell identification during human fibroblast reprogramming.
AuthorsQuintanilla RH, Asprer JS, Vaz C, Tanavde V, Lakshmipathy U
Journal
PubMed ID24416407
'Induced pluripotent stem cells (iPSCs) are promising tools for disease research and cell therapy. One of the critical steps in establishing iPSC lines is the early identification of fully reprogrammed colonies among unreprogrammed fibroblasts and partially reprogrammed intermediates. Currently, colony morphology and pluripotent stem cell surface markers are used to ... More
Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective.
AuthorsNavas-Moreno M, Mehrpouyan M, Chernenko T, Candas D, Fan M, Li JJ, Yan M, Chan JW,
JournalSci Rep
PubMed ID28667313
Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere ... More