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查看更多产品信息 DNA Binding Beads for MagMAX™ DNA Multi-Sample Ultra Kit - FAQs (A25562)
9 个常见问题解答
We have optimized protocols for extracting DNA from buccal swabs, whole blood, blood cards (Whatman FTA), oral rinse, saliva, and urine samples. In addition, the kit can be used to process vaginal microbiota samples (e.g., ThinPrep, Surepath) for gDNA extraction from yeast, bacteria (Gram positive/negative), viruses, and protozoa with the addition of additional enzymatic steps.
On rare occasions, the sample may turn cloudy after adding the Multi-Sample DNA Lysis Buffer. The sample will clear up when isopropanol is added. This will not have any impact on the yield or quality of the DNA.
Heparin is not recommended as an anti-coagulant since it can cause inhibition of PCR reactions. Instead, we strongly recommend collecting blood samples in EDTA or sodium citrate.
We recommend transferring the lysate to a new MagMAX Express-96 Deep Well Plate (Cat. No. 4388476). This option eliminates contamination risks and saves time. To transfer lysates, set a multi-channel micropipetor to ˜300 µL and transfer one row at a time. Each well should contain 200-250 µL after transfer.
Buccal swabs can be shipped at 25 degrees C or below. Exposure of the swabs to high temperatures (over 30 degrees C) during shipment has been associated with poor performance in the workflow. To prevent degradation, ensure that buccal swabs are completely dry before shipment. Place the swabs in a paper envelope for storage after collection - paper envelopes are permeable and allow the swabs to dry effectively.
We recommend using one of the following types of buccal swabs with foam tips:
- Puritan PurFlock Ultra Flocked Swabs (Fisher Scientific, Cat. No. 22-025-192)
- Puritan HydraFlock Swabs, standard tip (Puritan, Cat. No. 25-3306-H )
- Sterile Foam Tipped Swabs (Puritan, Cat. No. 25-1506 1PF)
- 4N6FLOQSwabs, regular tip (Thermo Fisher Scientific, Cat. No. 4473979)
Note: Please do not use cotton swabs, as they may contain PCR inhibitors.
DNA Elution Buffer 1 is at a basic pH to help maximize release of the gDNA from the magnetic beads and has to be neutralized by DNA Elution Buffer 2.
RNase A treatment is not critical for this workflow. RNA that might be present in the samples is likely to be degraded in the course of the experiment and should not interfere with downstream applications. For those sample types that might potentially have some RNA carryover, we have included the RNase A as part of the Bead mix as a precaution.
Yes, you can combine the two elution buffers prior to eluting the DNA; instead of having the instrument pause, requiring you to add DNA Elution Buffer 2, press ‘start' on the instrument - just be sure to combine them directly before adding to the elution plate and before putting the plate on the KingFisher instrument (you do not want them to sit as a mix for too long). For example, if you wish to elute in 50 µL of buffer, you can mix 25 µL of DNA Elution Buffer 1 and 25 µL of DNA Elution Buffer 2, then add the total 50 µL into a well and run the protocol as you normally would.