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Fetal Bovine Serum, exosome-depleted - FAQs

查看更多产品信息 Fetal Bovine Serum, exosome-depleted - FAQs (A25904DG)

12 个常见问题解答

哪些细胞可以培养于去外泌体的FBS中？我又应该使用何种FBS浓度？

我们专门测试了Jurkat、MCF-7、HeLa、PC3、HEK293与A549细胞，所有这些种类的细胞都生长良好。HeLa细胞对于去外泌体FBS的存在最为敏感。您可能需要对去外泌体FBS的浓度进行调整，以匹配特定细胞系与培养系统的需要。我们推荐您以10%做为去外泌体FBS应用的起始浓度，在需要时进行上调或下调。同时，去外泌体FBS通过了单次传代后48小时的培养测试和验证（我们也成功完成了对HeLa、MCF-7和HEK293等细胞系长达96小时的培养测试）。

去外泌体FBS是如何生产的？

我们研发了一套专利工艺，以在去除外泌体的同时最大限度地保留血清的促生长性能。

我是否能够对去外泌体FBS进行解冻和重新冻存？

是的，相关操作均通过了测试。您可在解冻本品后，对其进行分装，再重新冻存。

在去外泌体FBS产品中，“去（depleted）”是什么程度？

在我们为您提供的去外泌体FBS产品中，包含≤10%的起始原料的FBS中外泌体成分。我们使用了一项专为定量外泌体而开发的内部荧光分析方案，以确定每批产品中外泌体的清除水平。

去外泌体FBS的品质与其他FBS产品相比有何区别？

与我们其他的血清产品一样，去外泌体的FBS也经过彻底的无菌测试。此外，本款产品还通过了外泌体清除测试和专门的细胞培养性能等附加测试。

我应何时使用去除了外泌体成份的FBS产品？

我们推荐您使用这款产品进行外泌体相关的分析，您也可在分析前用这款产品来培养细胞，以最大限度地降低细胞背景信号。我们推荐您优化使用浓度，最常用的浓度范围是5-10%的使用浓度。

What cells can be cultured in Exosome-Depleted FBS, and at what concentration?

We have specifically tested Jurkat, MCF-7, HeLa, PC3, HEK293, and A549 cells, all of which exhibited acceptable performance. HeLa cells showed the most sensitivity to the presence of Exosome-depleted FBS. Adjustments may be needed for the concentration of Exosome-depleted FBS to match the requirements of the specific cell line and system. We recommend starting at 10% Exosome-depleted FBS, and adjusting up or down from there as necessary. Also, the Exosome-depleted FBS has been tested and validated for a single passage of cells for 48 hours (and we have also tested HeLa, MCF-7, and HEK293 cells successfully up to 96 hours of culture).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is Exosome-depleted FBS manufactured?

We have developed a proprietary process to remove exosomes and maintain as much of the growth performance as possible.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw and refreeze Exosome-depleted FBS?

Yes, this product has been tested to ensure there is no loss of product performance after thawing, aliquoting, and refreezing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What does "depleted" in Exosome-depleted FBS mean?

The Exosome-depleted FBS that we ship to you will contain ≤10% of the exosomes present in the starting material FBS. We use an in-house fluorescence assay specifically developed for exosome quantification to confirm this level of depletion in every lot. See the scientific poster at thermofisher.com/exosomedepletedfbs

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there anything different about the quality of Exosome-depleted FBS compared to other FBS products?

Exosome-depleted FBS undergoes full sterility testing like our other sera products. It is subjected to additional tests for exosome depletion and a specific cell culture performance test.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When would I use Exosome-depleted FBS?

We recommend using this product for exosome-specific assays, but you may also use it to culture the cells prior to the assay to minimize background signaling in your cells. We recommend determining the concentration to use, but 5-10% FBS is the most common.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.