PowerUp™ SYBR™ Green Master Mix for qPCR, 10 x 1 mL - FAQs

The PowerUp SYBR Green Master Mix uses a Dual Lock Taq DNA Polymerase enzyme. This enzyme combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. As such, this mechanism provides excellent specificity.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

We have not tested PowerUp SYBR Green Master Mix for qPCR at -20 degrees C. We have only performed accelerated and real-time stability testing at 2-8 degrees C, as this is the recommended and most convenient storage temperature.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

The recommended storage temperature for the PowerUp SYBR Green Master Mix is 2-8 degrees C.

Both master mixes are compatible with all Applied Biosystems Real Time PCR instruments, but Power SYBR Green Master Mix can only be run in Standard Mode. PowerUp SYBR Green Master Mix contains a heat-labile UDG, which allows for review of PCR products up to 72 hrs post-PCR (unlike Power SYBR Green Master Mix). Finally, PowerUp SYBR Green Master Mix uses Dual-Lock Taq DNA Polymerase, which uses a combination of two hot start modifications for exceptional specificity.

PowerUp SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems Real-time PCR instruments (except the 7900HT Fast mode instrument). It is also compatible with the Bio-Rad iQ5, Roche LightCycler 480, and Agilent MX3005P systems.