人心肌细胞免疫细胞化学试剂盒

货号	数量
A25973	50 tests

货号 A25973

价格（CNY)

6,666.00

添加至购物车

50 tests

价格（CNY)

6,666.00

添加至购物车

人心肌细胞免疫细胞化学试剂盒能够实现两个关键心肌细胞标志物的较佳图像分析：NKX2.5 和 TNNT2/cTnT。它是一款能为心肌细胞提供优质成像的试剂盒，其包含一整套一抗和二抗、细胞核 DNA 染色剂以及所有用于优化染色实验的预制缓冲液。

使用人心肌细胞免疫细胞化学试剂盒，您可以：
•使用高特异性抗体确认关键心肌细胞标志物的表达
•一次测量多个标志物，即可获得更多样品信息
•轻松生成漂亮的多通路图像
•通过去除染色方案中不必要的洗涤步骤，可以节省时间并避免细胞损失
•使用一组完整且优化的 ICC 试剂可靠处理样品

确认关键心肌标志物的表达
此试剂盒中包含的抗体已经过精心选择和验证，能在心肌细胞的免疫细胞化学分析中实现高性能。抗体适用于使用 Gibco™ PSC 心肌细胞分化试剂盒对多能干细胞生成的心肌细胞进行表征。

获得更多样品信息
试剂盒中的抗体经设计能够对 NKX2.5 和 TNNT2 以及细胞核 DNA 染色同时进行特异性评估，有助于节省时间和珍贵样品。

生成漂亮的多通路图像
利用明亮、光稳定的 Alexa Fluor™ 染料的卓越性能，此试剂盒能够使用常见的蓝色、绿色和橙色/红色滤光片组采集心肌细胞标志物表达的漂亮多通路图像。

去除不必要的洗涤步骤
推荐用于此试剂盒的简化方法可去除标准免疫细胞化学方案中发现的不必要的洗涤步骤、节省时间并降低染色操作过程中细胞损失的可能性，特别是在使用玻璃盖玻片或腔室玻片时。唯一的洗涤步骤在与一抗和二抗孵育后进行。

可靠地处理样品
优化的预制固定、透化、封闭和洗涤缓冲液套件可以专注于回答科学问题，而不是花费时间准备试剂和确定兼容性。

您能获得什么
可轻松扩展至任何培养板、培养皿或玻片规格，人心肌细胞免疫细胞化学试剂盒所含试剂使用 200 µL 染色体积足以对50份样品进行染色。详细的试剂盒内容可在下方找到。

仅供科研使用。不可用于诊断程序。

产品类型免疫细胞化学试剂盒

数量50 tests

偶联物2 种颜色, Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594

产品线Alexa Fluor™

Unit SizeEach

内容与储存

在 -20°C 至 4°C 下储存：

10 μL 小鼠抗人 TNNT2 (1000X)
10 μL 兔抗人 NKX2.5 (1000x)
40 μL Alexa Fluor™ 555 驴抗兔 (250X)
40 μL Alexa Fluor™ 594 驴抗兔 (250X)
40 μL Alexa Fluor™ 488 驴抗小鼠 (250X)

储存在 -20°C 至室温下：

1小瓶 NucBlue™ 固定细胞 Fixed ReadyProbes™ 试剂 (1X)
10 mL 固定液 (1X)
10 mL 透化溶液 S (1X)
20 mL 封闭溶液 (1X)
20 mL 洗涤缓冲液 (10X)

常见问题解答 (FAQ)

我正在计划使用PSC心肌细胞分化试剂盒。我获得的分化细胞预期能够表达哪些标志物？

通过PSC心肌细胞分化试剂盒生成的心肌细胞经测试能够表达TNNT2，Nkx2.5，MYH6和α-辅肌动蛋白等关键标志物。心肌细胞免疫细胞化学试剂盒中包含了经验证过的抗体，能够检测用PSC心肌细胞分化试剂盒生成的培养物中TNNT2和Nkx2.5的表达。

The Human Cardiomyocyte Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Human Cardiomyocyte Immunocytochemistry Kit for flow cytometry analysis?

We do not recommend using the Human Cardiomyocyte Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer the individual components of the Human Cardiomyocyte Immunocytochemistry Kit as standalone products?

Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am planning to use the PSC Cardiomyocyte Differentiation Kit. Which markers can I expected my differentiated cells to express?

Cardiomyocytes generated using PSC Cardiomyocyte Differentiation Kit have been tested for key markers such as TNNT2, Nkx2.5, MYH6, and Alpha-Actinin. The Cardiomyocyte Immunocytochemistry Kit (Cat. No. A25973) contains validated antibodies to measure TNNT2 and Nkx2.5 in cultures generated using PSC Cardiomyocyte Differentiation Kit.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.