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View additional product information for Mini Gel Tank - FAQs (A25977)
41 product FAQs found
小型转印模块专为小型凝胶电泳槽设计。该模块也适用于Bolt小型凝胶电泳槽（2014年12月31日起停产），但不适用于XCell SureLock Mini Cell或其他供应商生产的电泳槽。
由于通用的电极设计，小型转印模块可装在小型凝胶电泳槽的任意一侧。
是的，我们提供小型转印模块（货号B1000），适用于小型凝胶电泳槽。该转印模块也可用于Bolt小型凝胶电泳槽（2014年12月31日起停产）。请注意，Bolt小型转印模块（2014年12月31日起停产）对Bolt小型凝胶电泳槽和小型凝胶电泳槽均适用。
Bolt Bis-Tris Plus凝胶也可使用XCell SureLock Mini Cell、iBlot干转系统或Invitrogen半干转仪进行转印。
我们建议在将凝胶盒放置在电泳槽中之前，使用记号笔在凝胶盒上标记出孔的底部。
以下是可能原因和解决方案：
- 凝胶盒底部留有胶带。应从凝胶盒底部去除胶带。
- 与电源连接不良。使用电压表检测所有连接处的电导率。
- 缓冲液不足。应确保电泳槽中的缓冲液足以浸没凝胶的上样孔。
以下是可能原因和解决方案：
- 使用了浓度过高或错误的缓冲液。应检查缓冲液配方；必要时，稀释或重新配制。
- 电流设置过高。将电流降低至推荐的电泳条件（见使用手册第8页）。
以下是可能原因和解决方案：
- 缓冲液稀释过度。检查缓冲液配方；必要时，重新配制。
- 缓冲液槽泄漏。确保凝胶盒夹安置稳固，垫片在原位，并且凝胶盒夹已锁定。
- 电流设置过低。设置正确的电流。
以下是我们可提供的小型凝胶电泳槽替换部件：
替换部件 - 货号
凝胶电泳槽 - B4478641
小型凝胶电泳槽盖 - A25944
小型凝胶电泳槽基座 - A25950
凝胶盒夹，左 - A25946
凝胶盒夹，右 - A25945
凝胶盒夹凸轮手柄装置 - A26732
我们建议：
•电泳完毕后，妥善处理电泳缓冲液。用水冲洗电泳槽以除去残留的缓冲液。
•用蘸过水的柔软非摩擦性无尘布清洁小型凝胶电泳槽的表面。
•不要使用强力洗涤剂或溶剂清洗装置。
小型凝胶电泳槽不兼容氯化烃（如氯仿）、芳香烃（如甲苯和苯）或丙酮。
在使用转接板的情况下，Precise Tris-HEPES凝胶可兼容XCell SureLock Mini Cell或小型凝胶电泳槽。
注意：使用XCell SureLock Mini Cell或小型凝胶电泳槽时，1块凝胶需要2个转接板，2块凝胶需要1个转接板。
我们的传统Bolt Bis-Tris Plus小型凝胶（货号BGxxxxxBOX，2014年12月31日起停产）只能使用Bolt小型凝胶电泳槽（2014年12月31日起停产，库存售尽后将停止供应）进行电泳。
我们的新型Bolt Bis-Tris Plus小型凝胶（货号NWxxxxxBOX）以及Invitrogen小型凝胶和NuPAGE小型凝胶，可使用小型凝胶电泳槽或XCell SureLock Mini-Cell。若这些凝胶使用Bolt小型凝胶电泳槽（2014年12月31日起停产）进行电泳，则有必要升级电泳槽，可将黑色10.5 cm凝胶盒夹凸轮手柄更换为灰色10 cm凝胶盒夹凸轮手柄（货号A26732，凝胶盒夹凸轮手柄装置）。点击此处（https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/electrophoresis-chambers/mini-gel-tank/bolt-mini-gel-tank-resources.html）或视频（https://www.youtube.com/watch?v=1FtiX8Skllw），可查看凸轮手柄更换说明。
我们的中型凝胶可使用XCell4 SureLock Midi-Cell进行电泳。
以下是出现波浪形染色条带的可能原因和解决方案：
1. 缓冲槽内外的缓冲液高度不同：两个电泳槽中的缓冲液必须全都加至电极的高度。这样不仅可以防止缓冲液从内向外泄漏，还能起到散热的作用，从而防止出现波浪形染色条带。
2. 所用电泳缓冲液稀释度大于1X：我们推荐使用1X电泳缓冲液。
3. 使用旧的电泳缓冲液：应确保使用新鲜的电泳缓冲液，并且不要重复利用电泳缓冲液。
可能是因为Bolt凝胶盒插反了（大塑料板朝前，上样孔朝后），尽管这很难实现。当凝胶反向插入时，电流从凝胶底端流向顶端，导致样品反向电泳。对调导线将转换凝胶电泳方向，但会导致电流从阳极流向阴极。阴极是由带有铂涂层的不锈钢制成，阳极是由铂丝制成。电子从阳极流向阴极，会导致钢芯生锈。但是，当正确连接导线时，电子从阴极流向阳极，铂丝作为电子受体则不会生锈。
注意：当凝胶盒正确插入时，凝胶盒上的印刷字体（凝胶类型、SKU和有效期）是从左往右读的（请参见说明书第11页（https://tools.thermofisher.com/content/sfs/manuals/mini_gel_tank_man.pdf)）。
以下是可能原因和解决方案：
1.使用了浓度过高或错误的缓冲液：检查缓冲液配方；必要时，稀释或重新配制
2. 电流设置过高：将电源条件降低至推荐的电泳条件
以下是可能原因和解决方案：
1. 凝胶盒底部留有胶带：从凝胶盒底部移除胶带。
2. 未完全与电源连接：使用电压表检测所有连接处的电导率。
3. 缓冲液不足：确保电泳槽中的缓冲液足以浸没凝胶上的上样孔。
DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Yes, NativePAGE gels are compatible with our Mini Gel tank, however there is a small variation from the original protocol.
The following protocol are for using NativePage gels with the Mini Gel Tank depending on if you are also performing a western transfer or not:
If you are NOT performing a western transfer:
1. Prepare 250 mL of each buffer (Anode and Dark Blue Cathode) per gel
2. After inserting a loaded NativePAGE gel into the Mini Gel Tank and pulling the clamp forward, fill the front cathode buffer chamber to the Fill Line with Dark Blue Cathode Buffer (~200 mL per gel)
3. Add 220 mL of Anode Buffer to the back anode buffer chamber for that gel. Start the gel run
If you are performing a western transfer:
1. Prepare 250 mL of Dark Blue Cathode Buffer, 250 mL of Light Blue Cathode Buffer, and 500 mL of Anode Buffer per gel
2. After inserting a loaded NativePAGE gel into the Mini Gel Tank and pulling the clamp forward, fill the front cathode buffer chamber to the Fill Line with Dark Blue Cathode Buffer (~200 mL per gel
3. Add 220 mL of Anode Buffer to the back anode buffer chamber for that gel
4. Start the gel run; pause the run after the dark blue dye has run ~1/3 of the way through gel
a. Pour out the buffers from the Mini Gel Tank
b. Refill the back anode buffer chamber with 220 mL of Anode Buffer per gel
c. Fill the front cathode buffer chamber to the Fill Line with Light Blue Cathode Buffer (~200 mL per gel)
5. Resume the gel run
Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .
We recommend keeping the cassette clamp out and leaving it dry/empty. So, do not fill any running buffer in the second chamber.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
No. Bio-Rad gels are of a different size (length, width, and depth) and have a special feature that precludes them from fitting in the Mini Gel Tank.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
All Novex, NuPAGE, and Bolt mini gels can be run in the Mini Gel Tank, including:
- NuPAGE Tris-Acetate Protein Gels
- 10 cm Bolt Bis-Tris Plus Gels
- NuPAGE Bis-Tris Protein Gels
- Novex Tricine Protein Gels
Note: Refer to the user manual for specific run conditions for each gel type.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Mini Gel Tank includes the Runner Tank, Cassette Clamps (left & right), Tank Base, Tank Lid, and Power Supply Adapters.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Mini Blot Module is designed exclusively for the Mini Gel Tank. It will also fit in the Bolt Mini Gel Tank (discontinued as of December 31, 2014) but will not fit in the XCell SureLock Mini Cell or other vendors' electrophoresis tanks.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Due to the universal electrode design, the Mini Blot Module fits on either side of the Mini Gel Tank.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Yes, we offer the Mini Blot Module (Cat. No. B1000), designed to be used with the Mini Gel Tank. This blot module will also work with the Bolt Mini Gel Tank (discontinued as of December 31, 2014). Please note that the Bolt Mini Blot Module (discontinued as of December 31, 2014) is also compatible with both the Bolt Mini Gel Tank and the Mini Gel Tank.
Bolt Bis-Tris Plus gels can also be transferred using the XCell SureLock Mini Cell, iBlot Dry transfer system, or using the Invitrogen Semi-Dry Blotter.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend marking the cassette at the bottom of the wells with a marker pen prior to placing the cassette in the electrophoresis tank.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Tape left on the bottom of the cassette. Remove tape from bottom of cassette.
- Connection to power supply not complete. Check all connections with a voltmeter for conductance.
- Insufficient buffer level. Make sure there is sufficient buffer in the electrophoresis tank to cover the wells of the gel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Buffers are too concentrated or incorrect. Check buffer recipe; dilute or re-make if necessary.
- Current is set at a higher limit. Decrease current to recommended running conditions (see Page 8 of the manual).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
- Buffers are too dilute. Check buffer recipe; remake if necessary.
- Buffer chamber is leaking. Make sure the cassette clamp is firmly seated, the gaskets are in place and the cassette clamp is locked.
- Current is set too low. Set correct current.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are the replacement parts we offer for the Mini Gel Tank:
Replacement part - Cat. No.
Gel Runner Tank - B4478641
Mini Gel Tank Lid - A25944
Mini Gel Tank Base - A25950
Cassette Clamp, left - A25946
Cassette Clamp, right - A25945
Cassette Clamp Cam Handle Set - A26732
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are our recommendations:
- When electrophoresis is complete, dispose of the buffer appropriately. Rinse the tank with water to remove residual buffer.
- Clean the surface of the Mini Gel Tank with a soft non-abrasive, lint-free cloth dampened with water.
- Do not use harsh detergents or solvents to clean the unit.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Mini Gel Tank is not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene) or acetone.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Precise Tris-HEPES gels are compatible with the XCell SureLock Mini Cell or Mini Gel Tank when used with adaptor plates.
Note: Two adaptor plates are required when running just one gel and one adaptor plate is required when running two gels using the XCell SureLock Mini Cell or Mini Gel Tank.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Our Original Bolt Bis-Tris Plus Mini gels (Cat. No. BGxxxxxBOX, discontinued as of December 31, 2014) can only be run in the Bolt Mini Gel Tank (discontinued as of December 31, 2014, and will be offered until inventory is depleted).
Our New Bolt Bis-Tris Plus Mini gels (Cat. No. NWxxxxxBOX), as well as our Invitrogen Mini gels and NuPAGE Mini gels can be run using the Mini Gel Tank, or XCell SureLock Mini-Cell. To run these gels using the Bolt Mini Gel Tank (discontinued as of December 31, 2014), upgrading of the tank is necessary by replacing the black 10.5 cm cassette clamp cam handles with gray 10 cm cassette clamp cam handles (Cat. No. A26732, Cassette Clamp Cam Handle Set). Instructions for replacement of the cam handles can be found here (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/mini-gel-tank/resources-upgrading-bolt-mini-gel-tank.html).
Our Midi gels can be run using the XCell4 SureLock Midi-Cell.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are some causes and solutions for wavy dye fronts:
1) Difference in buffer level between the inner and outer buffer chambers: Both buffer chambers must be filled up to the electrode with wells completely covered. This will not only prevent leaks from the inside to the outside but will also act a heat sink and prevent wavy dye fronts.
2) Using running buffer that was diluted more than 1X: We recommend using 1X running buffer.
3) Using old running buffer: Make sure that the running buffer is fresh and don't reuse the running buffer.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
It is likely that the Bolt gel cassette was inserted backwards into the unit (large plate facing the front and the wells facing the back) even though this is pretty difficult to do. When the gel is inserted backwards, the current flows from the bottom of the gel to the top, resulting in the samples running in the opposite direction. Reversing of the leads will switch the direction of the gel run, however, this will cause the current to flow from the anode to the cathode. The cathode electrode is made of stainless steel with platinum coating, and the anode electrode is made of platinum wire. Flow of electrons from the anode to the cathode will result in rusting of the steel core. On the other hand, when the leads are connected properly, the electrons flow from the cathode to the anode and the recipient of the electrons is the platinum wire that does not rust.
Note: When the Bolt gel cassette is inserted properly into the Bolt Mini Gel Tank or Mini Gel Tank, the lettering (gel type, SKU and expiration date) printed on the gel cassette reads from left to right (please see Page 11 of the manual (https://tools.thermofisher.com/content/sfs/manuals/mini_gel_tank_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
1) Buffers are too concentrated or incorrect: Check buffer recipe; dilute or remake if necessary
2) Current is set at a higher limit: Decrease current to recommended running conditions.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Here are possible causes and solutions:
1) Tape left on the bottom of the cassette: Remove tape from bottom of cassette.
2) Connection to power supply not complete: Check all connections with a voltmeter for conductance.
3) Insufficient buffer level: Make sure there is sufficient buffer in the electrophoresis tank to cover the wells of the gel.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.