Ion 16S™ Metagenomics 试剂盒设计用于使用 Ion Torrent™ 半导体测序工作流程快速、全面和广泛地分析混合微生物群。本试剂盒能够对细菌中 16S了解更多信息
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货号
数量
A26216
100 reactions
货号 A26216
价格(CNY)
13,442.00
Each
添加至购物车
数量:
100 reactions
价格(CNY)
13,442.00
Each
添加至购物车
Ion 16S™ Metagenomics 试剂盒设计用于使用 Ion Torrent™ 半导体测序工作流程快速、全面和广泛地分析混合微生物群。本试剂盒能够对细菌中 16S rDNA 基因的高变区进行 PCR 扩增。然后在 Ion PGM™ 平台上使用 Ion PGM™ 测序 400 试剂盒对扩增片段进行测序,并使用 Ion Reporter™ 软件内的 Ion 16S™ 宏基因组学分析模块进行分析。
What is the Ion 16S Metagenomics Kit designed for?
The Ion 16S Metagenomics Kit is designed for rapid analyses of polybacterial samples using Ion Torrent Sequencing technology. The kit includes two primer sets that selectively amplify the corresponding hypervariable regions of the 16S region in bacteria:
- Primer set V2-4-8
- Primer set V3-6, 7-9
Sequence the amplified fragments on the Ion PGM System and analyze the results with the Ion Reporter software Ion 16S Metagenomics Kit analyses module. The combination of the two primer pools allows for sequence-based identification of a broad range of bacteria within a mixed population.
I have run out of Environmental Master Mix v2.0 from the Ion 16S Metagenomics Kit (Cat. No. A26216). Do you offer this as a standalone item?
Yes. You can purchase the TaqMan Environmental Master Mix 2.0 (Cat. No. 4396838).
How many reactions can be performed with the Ion 16S Metagenomics Kit (Cat. No. A26216)?
For each sample, the protocol involves two PCR reactions, one with primer set V2-4-8 and one with primer set V3-6.7. However, the kit contains sufficient master mix for 100 reactions and each of the two primer pools has sufficient volume for 100 reactions. Thus, the kit contains sufficient reagents for 100 reactions/50 samples.
Note: 100 reactions is dependent upon on the master mix, which is the limiting reagent. As far as primers are concerned, there is sufficient volume to process another 50 samples.
How many PCR reactions can I perform using the 2X Environmental Master Mix within the Ion 16S Metagenomics Kit (Cat. No. A26216)?
It is sufficient for 100 PCR reactions.
Can I use the Ion 540 Kit - Chef to prepare samples from the Ion 16S Metagenomics Kit?
No, the Ion 16S Metagenomics Kit utilizes 400 bp reads, so it is incompatible with the Ion 540 Kit - Chef, which only yields 200 bp reads.