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View additional product information for RevitaCell™ Supplement (100X) - FAQs (A2644501)
31 product FAQs found
PSC冻存试剂盒包含了无异源成份的PSC冻存培养基——该产品是用于冻存早期传代的多能干细胞（PSC）的即用型溶液，以及Gibco RevitaCell添加剂（100X）——应用于解冻后培养基的化学成份明确的复苏添加剂。这些试剂组合使用时，有助于将细胞活力的丧失降至最小，使细胞复苏后的恢复最大化，并尽可能减少PSC不期望的分化情况发生。本试剂盒也可用于冻存和复苏外周血单核细胞（PBMCs ），增强其复苏后的细胞活力及恢复情况。
是的，这一应用通过了iPSC测试。不过，原代细胞未进行相关测试。
不可以，这样的预处理对细胞有害。
不可以。Gibco RevitaCell添加剂与传统的ROCK抑制剂联用时会对PSC造成不良影响。
我们尚未测试此种应用。
Gibco RevitaCell添加剂经测试能够兼容Gibco Geltrex基质，Gibco玻连蛋白，LN-521干细胞基质和iMatrix-511。
两种应用均通过了相关测试。
我们仅在下列人类细胞（角膜上皮细胞，角质形成细胞，外周血单核细胞）和大鼠皮层神经元上进行了测试。
我们仅在人源PSC上进行了测试。
可以，Gibco Essential 8 Flex培养基能够兼容单细胞传代操作。当您进行单细胞传代时，请确保使用了Gibco RevitaCell添加剂（货号 A2644501）或一些其他种类的ROCK抑制剂。
可以，Gibco Essential 8 Flex培养基中可添加ROCK抑制剂。我们推荐您使用Gibco RevitaCell添加剂（货号 A2644501），该产品专为最大程度地减少应激对PSC的影响而设计。
Gibco Essential 8 Flex培养基与其他许多PSC培养基一样，在使用乙二胺四乙酸溶液成集落传代PSC细胞时，无需加入ROCK抑制剂。如果进行单细胞传代，我们则推荐添加Gibco RevitaCell添加剂（货号 A2644501）或其他ROCK抑制剂。
No. Combination of RevitaCell Supplement with a traditional ROCK inhibitor will result in deleterious effects on PSCs.
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Yes, it is normal that the RevitaCell Supplement has a strong smell.
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The CTS PSC Cryopreservation Kit (Cat. No. A4239301) provides the same efficient recovery, consistency, and reduced variability as the PSC Cryopreservation Kit (Cat. No. A2644601). However, these two kits have different intended use statements, i.e., “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals” and “For Research Use Only”, respectively. Furthermore, the CTS PSC Cryopreservation Kit has full USP sterility testing info and a Drug Master file (DMF), whereas the sterility testing of the PSC Cryopreservation is via a modified USP method, and it does not have a DMF.
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Yes. We have seen compatibility with the following differentiation kits provided by Thermo Fisher Scientific: PSC Cardiomyocyte Differentiation Kit (Cat. No. A2921201), PSC Definitive Endoderm Induction Kit (Cat. No. A3062601), PSC Neural Induction Medium (Cat. No. A1647801), and PSC Dopaminergic Neuron Differentiation Kit (Cat. No. A3147701).
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We recommend use of the Neon Electroporation device for electroporation of PSCs with Cas9 protein:guide RNA complex following the protocol guidance in the following demonstrated protocol (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/PG1584-PJT1313-COL31227-Demonstrated-Protocol-Performing-CRISPR-Cas9-FHR.pdf); see the section entitled “Knockout by electroporation of RNP using the Neon Transfection System.” We have seen that the Neon electroporation protocols 7 and 14 provide optimal indel formation while maintaining cell survival. However, the electroporation conditions may need to be optimized for your pluripotent cell line.
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Yes. The main reason that the workflow states this is to accommodate ROCK inhibitor or RevitaCell Supplement that have been added during passaging. In such circumstances where ROCK inhibitor or RevitaCell Supplement are added, you should feed your cells within 18-24 hours post-passaging. However, if ROCK inhibitor or RevitaCell Supplement are not included at the time of passaging, then you may skip the day post-passaging.
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The use of ROCK inhibitor is not required when culturing in StemFlex Medium on rhLaminin-521. However, supplementation with RevitaCell Supplement can provide additional support to PSCs during stressful transitions such as single-cell passaging.
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The PSC cryopreservation kit contains xeno-free PSC Cryopreservation Medium, which is a ready-to-use solution for the cryopreservation of early passage pluripotent stem cells (PSCs), and Gibco Revitacell Supplement (100X), a chemically defined recovery supplement for use in the post-thaw culture medium. When used in combination, these reagents help minimize loss of cell viability, maximize post-thaw recovery, and minimize unwanted differentiation of PSCs. This kit can also be used to cryopreserve and recover peripheral blood mononuclear cells (PBMCs) to improve post-thaw cell viability and recovery.
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Yes, this has been tested with iPSCs. However, testing on primary cells has not been performed.
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No, such pre-treatment results in deleterious effects on the cells.
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No. Combination of the Gibco RevitaCell Supplement with a traditional ROCK inhibitor results in deleterious effects on PSCs.
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We have not tested this application.
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Gibco RevitaCell Supplement been shown to be compatible with Gibco Geltrex matrix, Gibco Vitronectin, LN-521 Stem Cell Matrix, and iMatrix-511.
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Both types of cultures have been tested.
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We have only tested it with human species for the following cell types: corneal epithelial cells, keratinocytes, peripheral blood mononuclear cells, and rat cortical neurons.
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We have only tested it with human PSCs.
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Yes, Essential 8 Flex Medium is compatible with single cell passaging. Whenever you are performing single cell passaging, ensure that you use Gibco RevitaCell Supplement (Cat. No. A2644501) or another ROCK inhibitor.
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Yes, Essential 8 Flex Medium has been used with ROCK inhibitors. We suggest using Gibco RevitaCell Supplement (Cat. No. A2644501), which has been specifically designed to minimize the impact of stress on PSCs.
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As with many other PSC media, ROCK inhibitor is not required with Essential 8 Flex Medium when Gibco Versene Solution is used to passage PSCs as clusters. If cells are plated as singlets, we suggest that Gibco RevitaCell Supplement (Cat. No A2644501) or another ROCK inhibitor be used.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.