Simulated GABA synaptic input and L-type calcium channels form functional microdomains in hypothalamic gonadotropin-releasing hormone neurons.
AuthorsHemond PJ, O'Boyle MP, Roberts CB, Delgado-Reyes A, Hemond Z, Suter KJ,
JournalJ Neurosci
PubMed ID22745478
'Hypothalamic gonadotropin-releasing hormone (GnRH) neurons integrate the multiple internal and external cues that regulate sexual reproduction. In contrast to other neurons that exhibit extensive dendritic arbors, GnRH neurons usually have a single dendrite with relatively little branching. This largely precludes the integration strategy in which a single dendritic branch serves ... More
Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases.
AuthorsCaron E, Hall A
JournalScience
PubMed ID9831565
'The complement and immunoglobulin receptors are the major phagocytic receptors involved during infection. However, only immunoglobulin-dependent uptake results in a respiratory burst and an inflammatory response in macrophages. Rho guanosine triphosphatases (molecular switches that control the organization of the actin cytoskeleton) were found to be essential for both types of ... More
Improved immunocytochemical detection of biotinylated probes with Neutralite avidin.
Authorsvan Roy N, Mangelschots K, Speleman F
JournalTrends Genet
PubMed ID8488564
Neurogenesis in the neocortex of adult primates.
AuthorsGould E, Reeves AJ, Graziano MS, Gross CG
JournalScience
PubMed ID10521353
In primates, prefrontal, inferior temporal, and posterior parietal cortex are important for cognitive function. It is shown that in adult macaques, new neurons are added to these three neocortical association areas, but not to a primary sensory area (striate cortex). The new neurons appeared to originate in the subventricular zone ... More
Multicolor immunofluorescence and flow cytometry utilizing cascade blue to purify murine hematopoietic stem cells from fetal liver and bone marrow.
AuthorsDonahue CJ, Fennie C, Villacorta R, La H, Lasky LA, Ohneda O
JournalCytometry
PubMed ID10451507
BACKGROUND: Here we demonstrate the utility of cascade blue (CB), to purify hematopoietic stem cells by flow cytometry. Multicolor immunofluorescence and the sensitivity (signal-to-noise) of the fluorochromes are essential for the identification and isolation of rare stem cell populations. METHODS: We isolated hematopoietic stem cells utilizing a 407 nm laser ... More