HepExtend™ Supplement (50X) - FAQs

到目前为止，Gibco HepExtend添加剂仅在冻存的人体肝细胞上进行过测试；因此我们不推荐将此添加剂用于其他物种。

我们推荐使用Gibco HepExtend添加剂保持肝细胞的培养时间可长达10天，而且经测试对80%左右的人体批次均有效——其中包括代谢-合格，诱导-合格和转运体-合格的批次。

Gibco HepExtend添加剂能够兼容目前的肝细胞培养方法及相关设备，因此我们推荐您按照网站上所提供的Gibco冻存和培养冻存肝细胞实验方案来开展实验。

Gibco HepExtend添加剂不含生长因子、细胞因子、FBS或药物小分子。不过，其中含有少量的BSA（50X，0.12375g/mL）。

我们推荐Gibco HepExtend添加剂的冻融次数不要超过两次。因此，我们推荐化冻Gibco HepExtend添加剂后即分装为一次性使用的单管，再保存于–20°C至–5°C的非除霜型冰箱中。

Gibco HepExtend培养基试剂盒包含了配制培养肝细胞所需的完全培养基的所有成份如下：

- Gibco HepExtend添加剂（货号A2737501）
- Williams E培养基，500mL（货号A1217601）
- 肝细胞维持添加剂包（货号CM4000）

HepExtend Supplement (50X) is an enriched supplement designed to enhance the culture lifespan (cell viability, function, and number of days in culture) of cryopreserved primary hepatocytes. We recommend using the supplement in conjunction with William's E Medium (Cat. No. A1217601) and Hepatocyte Maintenance Supplement Pack (Cat. No. CM4000), following the recommended hepatocyte culture methods. When cultured in the presence of the HepExtend Supplement, primary human hepatocyte lots will live and be functional for 10 days or longer depending on the lot of cells and other assay conditions. HepExtend Supplement does not contain growth factors, cytokines, fetal bovine serum, or pharmaceutical small molecules that are known to interfere with primary hepatocyte function.

At this time, Gibco HepExtend Supplement has only been tested on cryopreserved human hepatocytes; we therefore cannot recommend using this supplement on other species.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend that Gibco HepExtend Supplement be used to keep hepatocytes in culture for up to 10 days, and it has been shown to work on approximately 80% of human lots tested-including metabolism-qualified, induction-qualified, and transporter-qualified lots.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Gibco HepExtend Supplement is compatible with current hepatocyte culturing methods and instrumentation and therefore, we recommend following the Gibco Thawing and Plating Cryopreserved Hepatocytes protocol that is available on our website.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Gibco HepExtend Supplement does not contain growth factors, cytokines, FBS, or pharmaceutical small molecules. However, it does contain small amounts of BSA (0.12375 g/mL at 50X).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

We recommend putting the Gibco HepExtend Supplement through no more than two freeze/thaw cycles. Therefore, we recommend that Gibco HepExtend Supplement be thawed and divided into single-use aliquots, which are then stored in a non-frost-free freezer at -20 degrees C to -5 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The HepExtend Media Kit contains the following components needed to make the complete medium for hepatocyte culture:

- HepExtend Supplement (50X) (Cat. No.A2737501)
- Williams E Medium (500 mL) (Cat. No.A1217601)
- Primary Hepatocyte Maintenance Supplements (Cat. No.CM4000)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.