Ion 540™ Chip Kit, 4 Chips - FAQs

View additional product information for Ion 540™ Chip Kit - FAQs (A27766, A27765)

8 product FAQs found

Can I use Ion 540 Chip Kit with Ion AmpliSeq Primary Immune Deficiency Research Panel v2? I am worried about the average amplicon length of 243 bp.

Yes, can use Ion 540 chips with an amplicon length of 243 bp. The 200 bp protocol, which is available for all chip sizes including Ion 540 and 550, can be used for amplicons up to 275 bp. Instead, the 400 bp protocol can be used only with Ion 510, 520, and 530 chips with amplicons lengths ranging between 125 and 375 bps.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the shipping and storage conditions for the Ion 540 chip?

The Ion 540 chip is shipped at room temperature and should be stored at room temperature.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I reuse the Ion 550 Chef Reagents and Ion 550 Chef Solutions cartridges for running a second chip separately from the first chip?

The Ion 550 Chef Reagents and Ion 550 Chef Solutions cartridges can be reused in a second single-chip templating run if the second run is performed within eight days of the first run.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which instrument platforms can I use with the Ion 540 chip?

The Ion 540 chip is compatible with the Ion GeneStudio S5/S5 Plus/Prime and Ion S5/S5 XL instruments.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I perform templating with either the Ion OneTouch 2 System or the Ion Chef System when using the Ion 540 chip for sequencing?

The Ion 540 chip workflow is supported with both the Ion OneTouch 2 System and the Ion Chef System for templating.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the largest length I can sequence with an Ion 540 chip?

Up to 200 bp libraries (500 flows) are supported on the Ion 540 chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reads can I expect with an Ion 540 chip?

You may expect 60-80 million reads with an Ion 540 chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the throughput of the Ion 540 Chip?

For 200 bp sequencing, it will yield 10-15 Gb. 400 bp sequencing is not supported on the Ion 540 Chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.