How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Proton System?
The number of samples that can be multiplexed in a single sequencing run depends on the capacity of the chip, the size of the library, and the required coverage. A table of approximate capacities based on the size of the library and chip can be found in the Ion Ampliseq Preparation User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0006735_AmpliSeq_DNA_RNA_LibPrep_UG.pdf) within the section “Strategies for combining Ion Ampliseq libraries.”
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
What is included in the Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle?
The Ion AmpliSeq Microbiome Health Research Kit, Ion 550 bundle (Cat. No. A46497) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, the IonCode Barcode Adapters, the Ion 550 Kit - Chef, and the Ion 550 Chip Kit for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 384 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
What is included in the Ion AmpliSeq Microbiome Health Research Kit, Library bundle?
The Ion AmpliSeq Microbiome Health Research Kit, Library bundle (Cat. No. A46495) consists of the Ion AmpliSeq Microbiome Health Research - 16S rRNA Gene Pool, the Ion AmpliSeq Microbiome Health Research - Target Species Pool, the Ion AmpliSeq Library Kit Plus, and the IonCode Barcode Adapters for the rapid preparation of barcoded sample libraries from bacterial DNA. Sufficient reagents are provided to prepare uniquely barcoded DNA libraries from 48 research samples.
Note: The Ion Library TaqMan Quantitation Kit (Cat. No. 4468802) must be purchased separately.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Can I use the AmpliSeq_Microbiome_Health_ Analysis plugin for Torrent Suite Software and Ion Reporter Software workflows for my custom 16S or target species AmpliSeq panel?
The Torrent Suite AmpliSeq_Microbiome_Health_ Analysis plugin and Ion Reporter Software workflows were developed and validated exclusively for the Ion AmpliSeq Microbiome Health Research Kit. Open source analysis tools may be available for your custom solutions.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Can I customize the list of target species for my microbiome diversity research project?
Customization solutions are available through AmpliSeq custom designs (Ampliseq.com), where you can customize your own assays for any species of your interest. For details, please contact your local Field Applications Scientist (FAS).
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
