MagMAX™ mirVana™ RNA Isolation Kits
Applied Biosystems™

MagMAX™ mirVana™ RNA Isolation Kits

The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types.
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货号产品类型数量
A2782896 次制备
A27828B1000Proteinase K960 Reactions
A27828B1001TURBO DNase960 Reactions
A27828B1002Lysis/Binding Enhancer960 Reactions
A27828B1003Proteinase K Digestion Buffer960 Reactions
A27828B1004RNA Binding Beads960 Reactions
A27828B1005TURBO DNase Buffer960 Reactions
A27828B1006Lysis Buffer960 Reactions
A27828B1007Rebinding Buffer960 Reactions
A27828B1008Wash Solution 2 Concentrate960 Reactions
A27828B1009Wash Solution 1 Concentrate960 Reactions
A27828B1010Elution Buffer960 Reactions
A278280Proteinase K96 Reactions
A278281TURBO DNase96 Reactions
A278282Lysis/Binding Enhancer96 Reactions
A278283Proteinase K Digestion Buffer96 Reactions
A278284RNA Binding Beads96 Reactions
A278285TURBO DNase Buffer96 Reactions
A278286Lysis Buffer96 Reactions
A278287Rebinding Buffer96 Reactions
A278288Wash Solution 2 Concentrate96 Reactions
A278289Wash Solution 1 Concentrate96 Reactions
A2782810Elution Buffer96 Reactions
货号 A27828
价格(CNY)
4,239.00
飞享价
Ends: 31-Dec-2026
5,480.00
共减 1,241.00 (23%)
Each
数量:
96 次制备
价格(CNY)
4,239.00
飞享价
Ends: 31-Dec-2026
5,480.00
共减 1,241.00 (23%)
Each

Scalable isolation of RNA (including small RNAs)

The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:

  • Automation-ready, phenol-free extraction
  • Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
  • Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays

One kit to isolate true total RNA from a diverse set of sample types

Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering 'specialized' kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:

  • Serum/plasma
  • Whole blood
  • Tissue
  • Cell culture
  • Urine

Benefits of MagMAX magnetic bead–based purification

Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.

For Research Use Only. Not for use in diagnostic procedures.
规格
洗脱体积20 to 100 μL
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于(应用)下一代测序、实时荧光定量 PCR、microRNA 分析
适用于(设备)KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
高通量能力高通量兼容性、手动方案、自动方案
纯化时间Up to 1.5 hr.
数量96 次制备
运输条件盒 1:冰
盒 2:室温
原始材料量Cells: ≤106
Plasma: ≤100 μL
Serum: ≤100 μL
Tissue: ≤50 mg
Urine: ≤250 μL
Whole Blood: ≤50 μL
产量Cells: ≤32 μg RNA per 50,000 cells
Tissue: ≤500 μg per 5 mg
分离技术Magnetic Bead
样品类型血液, 细胞, 液体样品(例如血清), RNA, 组织, Whole Blood
目标mRNA, siRNA, miRNA
Unit SizeEach
内容与储存

盒 1(在 -15 至 -25°C 下储存)
• 1 x 0.48 mL 蛋白酶 K、50 mg/mL
• 1 x 0.96 mL 裂解/结合增强剂
• 1 x 0.2 mL TURBO DNase™、20 U/μL

盒 2(在室温下储存)
• 1 x 115 mL 裂解缓冲液
• 1 x 4.4 mL PK 酶切缓冲液
• 1 x 2 mL RNA 结合珠
• 1 x 20 mL 洗涤液 1 浓缩液
• 1 x 60 mL 洗涤液 2 浓缩液
• 1 x 4.8 mL 再结合缓冲液
• 1 x 4.6 mL MagMAX™ TURBO DNase™ 缓冲液
• 1 x 9.6 mL 洗脱缓冲液
• 1 个处理板
• 2 个洗脱板
• 4 个板盖

常见问题解答 (FAQ)

在哪里可以找到使用MagMAX mirVana总RNA提取试剂盒进行自动化操作的脚本?

您可以在产品页面找到MagMAX mirVana总RNA提取试剂盒在MagMAX Express-96, KingFisher Flex, 或KingFisher Duo仪器上使用的脚本。

我可以将制备好用于MagMAX mirVana总RNA提取试剂盒的蛋白酶K消化液 (Proteinase K Digestion Mix) 储存并重复使用吗?

不行,我们建议每次都新鲜制备蛋白酶K消化液。

在开始MagMAX mirVana总RNA提取步骤之前,我可以将裂解后的样本保存在裂解结合缓冲液 (Lysis Binding Mix) 中吗?

可以,但是我们仅建议将样品在裂解结合缓冲液中于-20度储存不超过4天。在进行RNA纯化前应该将样本融化并平衡至室温。

MagMAX mirVana 总RNA分离试剂盒内带有板子吗?

是的,试剂盒内带有一块处理板,两块洗脱板,以及四个板盖,用于手动处理样本。但是,如果您希望使用KingFisher 仪器,那么您需要额外购买深孔板,标准板,以及磁棒套。

你们是否提供一个MagMAX RNA分离试剂盒,我可以用它来提取小RNA,比如miRNAs?

是的, Applied Biosystems的MagMAX mirVana总RNA分离试剂盒(货号A27828)能从各种样品(血清、血浆、血液、组织、细胞、尿液)中即可分离大片段RNA又可分离小RNA。< / p >