Panax ginseng C. A. Meyer is well known as traditional herbal medicine, and ginseng berries are known to exhibit potential immune-enhancing functions. However, little is known about the in vivo immunomodulatory activity of Korean ginseng berries. In this study, crude Korean ginseng berries polysaccharides (GBP) were isolated and their immunomodulatory ... More
Excessive manganese alters serum biochemical indices, induces histopathological alterations, and activates apoptosis in liver and cerebrum of Jianzhou Da'er goat (Capra hircus).
AuthorsXiang Y,Wang L,Wei Y,Zhang H,Emu Q
JournalComparative biochemistry and physiology. Toxicology & pharmacology : CBP
PubMed ID34752896
Identification of osteopontin as a urinary biomarker for autosomal dominant polycystic kidney disease progression.
AuthorsKim H,Sung J,Bae JY,Lee P,Oh YK,Kim H
JournalKidney research and clinical practice
PubMed ID35791741
BACKGROUND: Autosomal dominant polycystic kidney disease (ADPKD), one of the most common human monogenic diseases, is characterized by the presence of numerous fluid-filled renal cysts and is a leading cause of end-stage renal disease (ESRD). Urinary biomarkers may be useful for predicting the variable course of ADPKD progression from cyst ... More
Evidence for crosstalk between the aryl hydrocarbon receptor and the translocator protein in mouse lung epithelial cells.
Cellular homeostasis requires the use of multiple environmental sensors that can respond to a variety of endogenous and exogenous compounds. The aryl hydrocarbon receptor (AHR) is classically known as a transcription factor that induces drug metabolizing enzymes when bound to toxicants such as 2,3,7,8-tetrachlorodibenzo-Ï-dioxin (TCDD). The receptor has a growing ... More
AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology.
Mitochondria are dynamic organelles that undergo membrane remodeling events in response to metabolic alterations to generate an adequate mitochondrial network. Here, we investigated the function of mitochondrial fission regulator 1-like protein (MTFR1L), an uncharacterized protein that has been identified in phosphoproteomic screens as a potential AMP-activated protein kinase (AMPK) substrate. ... More
An improved workflow for accurate and robust healthcare environmental surveillance using metagenomics.
AuthorsShen J,McFarland AG,Blaustein RA,Rose LJ,Perry-Dow KA,Moghadam AA,Hayden MK,Young VB,Hartmann EM
JournalMicrobiome
PubMed ID36457108
BACKGROUND: Effective surveillance of microbial communities in the healthcare environment is increasingly important in infection prevention. Metagenomics-based techniques are promising due to their untargeted nature but are currently challenged by several limitations: (1) they are not powerful enough to extract valid signals out of the background noise for low-biomass samples, ... More
A high-throughput drug discovery pipeline to optimize kidney normothermic machine perfusion.
Kidney transplantation is the only definitive therapy for end-stage kidney disease. The shortage of organs for transplantation is the main limitation of this life-saving treatment. Normothermic machine perfusion (NMP) is a novel preservation technique with the potential to increase the number of transplantable kidneys through reducing delayed graft function and ... More
Expression of DNA-damage response and repair genes after exposure to DNA-damaging agents in isogenic head and neck cells with altered radiosensitivity.
AuthorsTodorovic V,Groselj B,Cemazar M,Prevc A,Zakelj MN,Strojan P,Sersa G
JournalRadiology and oncology
PubMed ID35390246
BACKGROUND: Increased radioresistance due to previous irradiation or radiosensitivity due to human papilloma virus (HPV) infection can be observed in head and neck squamous cell carcinoma (HNSCC). The DNA-damage response of cells after exposure to DNA-damaging agents plays a crucial role in determining the fate of exposed cells. Tightly regulated ... More
Generation of a human induced pluripotent stem cell line carrying the TYR c.575C>A (p.Ser192Tyr) and c.1205G>A (p.Arg402Gln) variants in homozygous state using CRISPR-Cas9 genome editing.
AuthorsLiu J,Black GC,Kimber SJ,Sergouniotis PI
JournalStem cell research
PubMed ID35933957
K(V)7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure.
AuthorsPeixoto-Neves D,Kanthakumar P,Afolabi JM,Soni H,Buddington RK,Adebiyi A
JournalAmerican journal of physiology. Renal physiology
PubMed ID35001664
K(V)7 channels, the voltage-gated K( ) channels encoded by KCNQ genes, mediate heterogeneous vascular responses in rodents. Postnatal changes in the functional expression of K(V)7 channels have been reported in rodent saphenous arteries, but their physiological function in the neonatal renal vascular bed is unclear. Here, we report that, unlike ... More
Spray-dried microparticles of encapsulated gefitinib for slow-release localized treatment of periodontal disease.
AuthorsBaldelli A,Koivisto L,Oguzlu H,Guo Y,Häkkinen L,Pratap-Singh A,Larjava H
JournalInternational journal of pharmaceutics
PubMed ID37364780
Dexamethasone Selectively Inhibits Detachment of Metastatic Thyroid Cancer Cells during Random Positioning.
AuthorsMelnik D,Cortés-Sánchez JL,Sandt V,Kahlert S,Kopp S,Grimm D,Krüger M
JournalCancers
PubMed ID36980530
SIMPLE SUMMARY: Metastasis is the most dangerous feature of advanced cancers. In vitro models of this process could help us to study and understand mechanisms that are not readily accessible in the human body. In our approach, we used random positioning cell cultures to induce cancer cells to spread and ... More
Leptin correlates with monocytes activation and severe condition in COVID-19 patients.
Excessive monocyte activation with the development of excessive or uncontrolled release of proinflammatory cytokines often results in host tissue injury and even death in patients with pneumonia caused by the 2019 novel coronavirus. However, the changes of cytokine profiles of coronavirus disease 2019 (COVID-19) patients, as well as the underlying ... More
CdTe-QDs Affect Reproductive Development of Plants through Oxidative Stress.
AuthorsWang J,Gong Y,Yan X,Han R,Chen H
JournalToxics
PubMed ID37505551
With the continuous development of industry, an increasing number of nanomaterials are widely used. CdTe-QDs is a nanomaterial with good optical properties, but its release into the natural environment may pose a potential threat. The toxicity of nanoparticles in plants is beginning to be questioned, and the effect on phytotoxicity ... More
Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis.
JournalAnimal nutrition (Zhongguo xu mu shou yi xue hui)
PubMed ID38033603
Developing effective strategies to prevent diarrhea and associated-gut disorders in mammals has gained great significance. Owing to the many health benefits provided by the commensal microbiota of the intestinal tract, such as against environmental perturbation, we explored the host phenotype-associated microbes and their probiotic potential. Based on the observations that ... More
Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma.
BACKGROUND: Metabolism reprogramming plays a vital role in glioblastoma (GBM) progression and recurrence by producing enough energy for highly proliferating tumor cells. In addition, metabolic reprogramming is crucial for tumor growth and immuneâescape mechanisms. Epidermal growth factor receptor (EGFR) amplification and EGFRâvIII mutation are often detected in GBM cells, contributing ... More
A PorX/PorY and σ(P) Feedforward Regulatory Loop Controls Gene Expression Essential for Porphyromonas gingivalis Virulence.
AuthorsJiang C,Yang D,Hua T,Hua Z,Kong W,Shi Y
JournalmSphere
PubMed ID34047648
The PorX/PorY two-component system in the periodontal pathogen Porphyromonas gingivalis controls the expression of the por genes, encoding a type IX secretion system, and the sigP gene, encoding sigma factor Ï(P). Previous results implied that PorX/PorY and Ï(P) formed a regulatory cascade because the PorX/PorY-activated Ï(P) enhanced the por genes, ... More
A synthetic probiotic engineered for colorectal cancer therapy modulates gut microbiota.
BACKGROUND: Successful chemoprevention or chemotherapy is achieved through targeted delivery of prophylactic agents during initial phases of carcinogenesis or therapeutic agents to malignant tumors. Bacteria can be used as anticancer agents, but efforts to utilize attenuated pathogenic bacteria suffer from the risk of toxicity or infection. Lactic acid bacteria are ... More
Denitrification Biokinetics: Towards Optimization for Industrial Applications.
AuthorsSuri N,Zhang Y,Gieg LM,Ryan MC
JournalFrontiers in microbiology
PubMed ID34025593
Denitrification is a microbial process that converts nitrate (NO(3)(â)) to N(2) and can play an important role in industrial applications such as souring control and microbially enhanced oil recovery (MEOR). The effectiveness of using NO(3)(â) in souring control depends on the partial reduction of NO(3)(â) to nitrite (NO(2)(â)) and/or N(2)O ... More
Infection, dissemination, and transmission efficiencies of Zika virus in Aedes aegypti after serial passage in mosquito or mammalian cell lines or alternating passage in both cell types.
AuthorsTalavera-Aguilar LG,Murrieta RA,Kiem S,Cetina-Trejo RC,Baak-Baak CM,Ebel GD,Blitvich BJ,Machain-Williams C
JournalParasites & vectors
PubMed ID34006306
BACKGROUND: Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) with an urban transmission cycle that primarily involves humans and Aedes aegypti. Evidence suggests that the evolution of some arboviruses is constrained by their dependency on alternating between disparate (vertebrate and invertebrate) hosts. The goals of this study are to compare ... More
An HLA-G/SPAG9/STAT3 axis promotes brain metastases.
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID36780531
Brain metastases (BM) are the most common brain neoplasm in adults. Current BM therapies still offer limited efficacy and reduced survival outcomes, emphasizing the need for a better understanding of the disease. Herein, we analyzed the transcriptional profile of brain metastasis initiating cells (BMICs) at two distinct stages of the ... More
The Perils of Navigating Activity-Dependent Alternative Splicing of Neurexins.
AuthorsLiakath-Ali K,Südhof TC
JournalFrontiers in molecular neuroscience
PubMed ID33767611
Neurexins are presynaptic cell-adhesion molecules essential for synaptic function that are expressed in thousands of alternatively spliced isoforms. Recent studies suggested that alternative splicing at splice site 4 (SS4) of Nrxn1 is tightly regulated by an activity-dependent mechanism. Given that Nrxn1 alternative splicing at SS4 controls NMDA-receptor-mediated synaptic responses, activity-dependent ... More
A urine-based DNA methylation assay to facilitate early detection and risk stratification of bladder cancer.
BACKGROUND: Current non-invasive tests have limited sensitivities and lack capabilities of pre-operative risk stratification for bladder cancer (BC) diagnosis. We aimed to develop and validate a urine-based DNA methylation assay as a clinically feasible test for improving BC detection and enabling pre-operative risk stratifications. METHODS: A urine-based DNA methylation assay ... More
Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry.
SIMPLE SUMMARY: Under the background of total prohibition, it has always been the goal of the broiler industry to improve the harm of necrotizing enteritis and the performance of broiler chickens. As a potential green and healthy feed additive, lauric acid monoglyceride and cinnamaldehyde have important research significance. The results ... More
MCPIP1/Regnase-1 Expression in Keratinocytes of Patients with Hidradenitis Suppurativa: Preliminary Results.
JournalInternational journal of molecular sciences
PubMed ID34298861
The pathogenesis of hidradenitis suppurativa (HS) is yet to be fully understood. However, inflammation is a key element in the development of skin lesions. The aim of this study was to evaluate the expression of monocyte chemotactic protein-1-induced protein-1 (MCPIP1) in the skin of patients suffering from HS. Skin biopsies ... More
Intracellular pH regulation: characterization and functional investigation of H(+) transporters in Stylophora pistillata.
AuthorsCapasso L,Ganot P,Planas-Bielsa V,Tambutté S,Zoccola D
JournalBMC molecular and cell biology
PubMed ID33685406
BACKGROUND: Reef-building corals regularly experience changes in intra- and extracellular H(+) concentrations ([H(+)]) due to physiological and environmental processes. Stringent control of [H(+)] is required to maintain the homeostatic acid-base balance in coral cells and is achieved through the regulation of intracellular pH (pH(i)). This task is especially challenging for ... More
Synergistic Zerovalent Iron (Fe(0)) and Microbiological Trichloroethene and Perchlorate Reductions Are Determined by the Concentration and Speciation of Fe.
AuthorsRangan SM,Mouti A,LaPat-Polasko L,Lowry GV,Krajmalnik-Brown R,Delgado AG
JournalEnvironmental science & technology
PubMed ID33151674
Suppression of cigarette smoke induced MMP1 expression by selective serotonin re-uptake inhibitors.
AuthorsAdam G,Shiomi T,Monica G,Jarrod S,Vincent A,Becky M,Tina Z,Jeanine D
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
PubMed ID34137477
Globally, COPD remains a major cause of disability and death. In the United States alone, it is estimated that approximately 14 million people suffer from the disease. Given the high disease burden and requirement for chronic, longâterm medical care associated with COPD, it is essential that new disease modifying agents ... More
Sensitive detection of colorectal cancer in peripheral blood by a novel methylation assay.
BACKGROUND: Colorectal cancer (CRC) is a leading cause of cancer-related deaths worldwide. Early detection of CRC can significantly reduce its mortality rate. Current method of CRC diagnosis relies on the invasive endoscopy. Non-invasive assays including fecal occult blood testing (FOBT) and fecal immunological test (FIT) are compromised by low sensitivity ... More
Prebiotic Effect of Berberine and Curcumin Is Associated with the Improvement of Obesity in Mice.
Berberine and curcumin, used as food additives or food supplements, possess interesting anti-inflammatory and antioxidant properties. We tested the potential protective effect of both phytochemicals in genetically obese mice and we determined whether these effects can be related to the modulation of gut functions and microbiota. Ob/ob mice were fed ... More
Macrophage DCLK1 promotes atherosclerosis via binding to IKKβ and inducing inflammatory responses.
Atherosclerosis is a chronic inflammatory disease with high morbidity and mortality rates worldwide. Doublecortinâlike kinase 1 (DCLK1), a microtubuleâassociated protein kinase, is involved in neurogenesis and human cancers. However, the role of DCLK1 in atherosclerosis remains undefined. In this study, we identified upregulated DCLK1 in macrophages in atherosclerotic lesions of ... More
Microbial Communities and Interactions of Nitrogen Oxides With Methanogenesis in Diverse Peatlands of the Amazon Basin.
AuthorsBuessecker S,Zamora Z,Sarno AF,Finn DR,Hoyt AM,van Haren J,Urquiza Muñoz JD,Cadillo-Quiroz H
JournalFrontiers in microbiology
PubMed ID34267733
Tropical peatlands are hotspots of methane (CH(4)) production but present high variation and emission uncertainties in the Amazon region. This is because the controlling factors of methane production in tropical peats are not yet well documented. Although inhibitory effects of nitrogen oxides (NO(x)) on methanogenic activity are known from pure ... More
Antineoplastic effects of pharmacological inhibitors of aurora kinases in CSF3R(T618I)-driven cells.
AuthorsParducci NS,Garnique ADMB,Lima K,Carlos JAEG,Fonseca NP,de Miranda LBL,de Almeida BO,Rego EM,Traina F,Machado-Neto JA
JournalBlood cells, molecules & diseases
PubMed ID37839173
Baicalein and Baicalin Inhibit SARS-CoV-2 RNA-Dependent-RNA Polymerase.
Coronavirus Disease 2019 (COVID-19) is a deadly emerging infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Because SARS-CoV-2 is easily transmitted through the air and has a relatively long incubation time, COVID-19 has rapidly developed into a global pandemic. As there are no antiviral agents for the ... More
Biological impact of sequential exposures to allergens and ultrafine particle-rich combustion aerosol on human bronchial epithelial BEAS-2B cells at the air liquid interface.
Desulfovibrio Bacteria Are Associated With Parkinson's Disease.
AuthorsMurros KE,Huynh VA,Takala TM,Saris PEJ
JournalFrontiers in cellular and infection microbiology
PubMed ID34012926
Parkinsonâs disease (PD) is the most prevalent movement disorder known and predominantly affects the elderly. It is a progressive neurodegenerative disease wherein α-synuclein, a neuronal protein, aggregates to form toxic structures in nerve cells. The cause of Parkinsonâs disease (PD) remains unknown. Intestinal dysfunction and changes in the gut microbiota, ... More
Human Nasal and Lung Tissues Infected Ex Vivo with SARS-CoV-2 Provide Insights into Differential Tissue-Specific and Virus-Specific Innate Immune Responses in the Upper and Lower Respiratory Tract.
The nasal mucosa constitutes the primary entry site for respiratory viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While the imbalanced innate immune response of end-stage coronavirus disease 2019 (COVID-19) has been extensively studied, the earliest stages of SARS-CoV-2 infection at the mucosal entry site have remained unexplored. Here, ... More
Urinary miRNA profiles discriminate between obstruction-induced bladder dysfunction and healthy controls.
Authorsvon Siebenthal M,Besic M,Gheinani AH,Akshay A,Lizun-Platoni S,Kunz N,Burkhard FC,Monastyrskaya K
JournalScientific reports
PubMed ID33986358
Urgency, frequency and incomplete emptying are the troublesome symptoms often shared between benign prostatic obstruction-induced (BLUTD) and neurogenic (NLUTD) lower urinary tract dysfunction. Previously, using bladder biopsies, we suggested a panel of miRNA biomarkers for different functional phenotypes of the bladder. Urine is a good source of circulating miRNAs, but ... More
Transcriptome-wide m(6)A methylome during osteogenic differentiation of human adipose-derived stem cells.
OBJECTIVES: Adipose-derived stem cells are frequently used for bone regeneration both in vitro and in vivo. N(6)-methyladenosine (m(6)A) is the most abundant post-transcriptional modification on eukaryotic RNAs and plays multifaceted roles in development and diseases. However, the regulatory mechanisms of m(6)A in osteogenic differentiation of human adipose-derived stem cells (hASCs) ... More
Comparative analysis of the carrot miRNAome in response to salt stress.
AuthorsSzymonik K,Klimek-Chodacka M,Lukasiewicz A,Macko-Podgórni A,Grzebelus D,Baranski R
JournalScientific reports
PubMed ID38057586
Soil salinity adversely affects the yield and quality of crops, including carrot. During salt stress, plant growth and development are impaired by restricted water uptake and ion cytotoxicity, leading to nutrient imbalance and oxidative burst. However, the molecular mechanisms of the carrot plant response to salt stress remain unclear. The ... More
Moderate DNA hypomethylation suppresses intestinal tumorigenesis by promoting caspase-3 expression and apoptosis.
Global DNA hypomethylation is a most common epigenetic alteration in human neoplasia. However, accumulative evidence shows that global DNA hypomethylation impacts tumorigenesis in a tissue-specific manner, promoting tumorigenesis in some but suppressing tumorigenesis in others including colorectal cancer. The underlying mechanisms, especially how DNA hypomethylation suppresses tumorigenesis, remain largely unknown. ... More
Mesenchymal stromal cell mitochondrial transfer to human induced T-regulatory cells mediates FOXP3 stability.
The key obstacle to clinical application of human inducible regulatory T cells (iTreg) as an adoptive cell therapy in autoimmune disorders is loss of FOXP3 expression in an inflammatory milieu. Here we report human iTreg co-cultured with bone marrow-derived mesenchymal stromal cells (MSCs) during short-term ex vivo expansion enhances the ... More
The sex-specific patterns of changes in hypothalamic-pituitary-gonadal axis during experimental autoimmune encephalomyelitis.
Host-specific legume-rhizobium symbiosis is strictly controlled by rhizobial type III effectors (T3Es) in some cases. Here, we demonstrated that the symbiosis of Vigna radiata (mung bean) with Bradyrhizobium diazoefficiens USDA110 is determined by NopE, and this symbiosis is highly dependent on host genotype. NopE specifically triggered incompatibility with V. radiata ... More
Establishment of an induced pluripotent stem cell line (ZZUSAHi002-A) derived from peripheral blood mononuclear cells of a healthy individual.
AuthorsLiu S,Niu S,Li Y,Xiong H,Li Y,Jian L,Zhang L
JournalStem cell research
PubMed ID32911326
Microbiota and functional analyses of nitrogen-fixing bacteria in root-knot nematode parasitism of plants.
BACKGROUND: Root-knot nematodes (RKN) are among the most important root-damaging plant-parasitic nematodes, causing severe crop losses worldwide. The plant rhizosphere and root endosphere contain rich and diverse bacterial communities. However, little is known about how RKN and root bacteria interact to impact parasitism and plant health. Determining the keystone microbial ... More
Differential fibrotic phenotypes of hepatic stellate cells within 3D liver organoids.
AuthorsBrovold M,Keller D,Soker S
JournalBiotechnology and bioengineering
PubMed ID32391915
Liver fibrosis occurs in most cases of chronic liver disease, which are a somewhat common, but also a potentially deadly group of diseases. In vitro modeling of liver fibrosis relies primarily on the isolation of in vivo activated hepatic stellate cells (aHSCs) and studying them in standard tissue culture dishes ... More
Thermotolerant coral symbionts modulate heat stress-responsive genes in their hosts.
AuthorsCunning R,Baker AC
JournalMolecular ecology
PubMed ID32585772
Dietary Coated Sodium Butyrate Ameliorates Hepatic Lipid Accumulation and Inflammation via Enhancing Antioxidative Function in Post-Peaking Laying Hens.
AuthorsMiao S,Li Y,Mu T,Wang X,Zhao W,Li R,Dong X,Zou X
JournalMetabolites
PubMed ID37233691
During the aging process of laying hens, hepatic oxidative stress damage and lipid accumulation are prone to occur, leading to the deterioration of egg quality and a decline in production properties. This research was designed to explore the effects of different levels of coated sodium butyrate (CSB) addition on oxidation ... More
Urolithin A Protects Chondrocytes From Mechanical Overloading-Induced Injuries.
AuthorsHe Y,Yocum L,Alexander PG,Jurczak MJ,Lin H
JournalFrontiers in pharmacology
PubMed ID34220525
Physiological mechanical stimulation has been shown to promote chondrogenesis, but excessive mechanical loading results in cartilage degradation. Currently, the underlying mechanotransduction pathways in the context of physiological and injurious loading are not fully understood. In this study, we aim to identify the critical factors that dictate chondrocyte response to mechanical ... More
Impaired Detoxification of Trans, Trans-2,4-Decadienal, an Oxidation Product from Omega-6 Fatty Acids, Alters Insulin Signaling, Gluconeogenesis and Promotes Microvascular Disease.
Omegaâ6 fatty acids are the primary polyunsaturated fatty acids in most Western diets, while their role in diabetes remains controversial. Exposure of omegaâ6 fatty acids to an oxidative environment results in the generation of a highly reactive carbonyl species known as trans, transâ2,4âdecadienal (ttâDDE). The timely and efficient detoxification of ... More
CGRP Regulates the Age-Related Switch Between Osteoblast and Adipocyte Differentiation.
JournalFrontiers in cell and developmental biology
PubMed ID34124062
Osteoporosis is a chronic age-related disease. During aging, bone marrow-derived mesenchymal stem cells (BMSCs) display increased adipogenic, along with decreased osteogenic, differentiation capacity. The aim of the present study was to investigate the effect of calcitonin gene-related peptide (CGRP) on the osteogenic and adipogenic differentiation potential of BMSC-derived osteoblasts. Here, ... More
AB-Kefir Reduced Body Weight and Ameliorated Inflammation in Adipose Tissue of Obese Mice Fed a High-Fat Diet, but Not a High-Sucrose Diet.
AuthorsChen YT,Hsu AH,Chiou SY,Lin YC,Lin JS
JournalNutrients
PubMed ID34202894
Consumption of different types of high-calorie foods leads to the development of various metabolic disorders. However, the effects of multi-strain probiotics on different types of diet-induced obesity and intestinal dysbiosis remain unclear. In this study, mice were fed a control diet, high-fat diet (HFD; 60% kcal fat and 20% kcal ... More
OsJAZ11 regulates phosphate starvation responses in rice.
AuthorsPandey BK,Verma L,Prusty A,Singh AP,Bennett MJ,Tyagi AK,Giri J,Mehra P
JournalPlanta
PubMed ID34143292
MAIN CONCLUSION: OsJAZ11 regulates phosphate homeostasis by suppressing jasmonic acid signaling and biosynthesis in rice roots. ABSTRACT: Jasmonic Acid (JA) is a key plant signaling molecule which negatively regulates growth processes including root elongation. JAZ (JASMONATE ZIM-DOMAIN) proteins function as transcriptional repressors of JA signaling. Therefore, targeting JA signaling by ... More
Genotypic and Phenotypic Diversity among Human Isolates of Akkermansia muciniphila.
The mucophilic anaerobic bacterium Akkermansia muciniphila is a prominent member of the gastrointestinal (GI) microbiota and the only known species of the Verrucomicrobia phylum in the mammalian gut. A high prevalence of A. muciniphila in adult humans is associated with leanness and a lower risk for the development of obesity ... More
RNAi of a Putative Grapevine Susceptibility Gene as a Possible Downy Mildew Control Strategy.
Downy mildew, caused by the oomycete Plasmopara viticola, is one of the diseases causing the most severe economic losses to grapevine (Vitis vinifera) production. To date, the application of fungicides is the most efficient method to control the pathogen and the implementation of novel and sustainable disease control methods is ... More
Functional genomics analysis identifies loss of HNF1B function as a cause of Mayer-Rokitansky-Küster-Hauser syndrome.
AuthorsThomson E,Tran M,Robevska G,Ayers K,van der Bergen J,Gopalakrishnan Bhaskaran P,Haan E,Cereghini S,Vash-Margita A,Margetts M,Hensley A,Nguyen Q,Sinclair A,Koopman P,Pelosi E
JournalHuman molecular genetics
PubMed ID36282544
MayerâRokitanskyâKüsterâHauser (MRKH) syndrome is a congenital condition characterized by aplasia or hypoplasia of the uterus and vagina in women with a 46,XX karyotype. This condition can occur as type I when isolated or as type II when associated with extragenital anomalies including kidney and skeletal abnormalities. The genetic basis of ... More
Fluoxetine inactivates STAT3/NF-κB signaling and promotes sensitivity to cisplatin in bladder cancer.
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which causes coronavirus disease-2019 (COVID-19), interacts with the host cell receptor angiotensin-converting enzyme 2 (hACE2) via its spike 1 protein during infection. After the virus sequence was published, we identified two potent antibodies against the SARS-CoV-2 receptor binding domain (RBD) from antibody libraries using ... More
Orai3 and Orai1 mediate CRAC channel function and metabolic reprogramming in B cells.
The essential role of store-operated Ca(2+) entry (SOCE) through Ca(2+) release-activated Ca(2+) (CRAC) channels in T cells is well established. In contrast, the contribution of individual Orai isoforms to SOCE and their downstream signaling functions in B cells are poorly understood. Here, we demonstrate changes in the expression of Orai ... More
SeNPs alleviates BDE-209-induced intestinal damage by affecting necroptosis, inflammation, intestinal barrier and intestinal flora in layer chickens.
AuthorsZhang Z,Shan J,Shi B,Dong B,Wu Q,Zhang Z
JournalEcotoxicology and environmental safety
PubMed ID37567103
Purinergic signaling during Marek's disease in chickens.
AuthorsAkbar H,Fasick JJ,Ponnuraj N,Jarosinski KW
JournalScientific reports
PubMed ID36739336
Purinergic receptors (PRs) have been reported as potential therapeutic targets for many viral infections including herpesviruses, which urges the investigation into their role in Marekâs disease (MD), a herpesvirus induced cancer in chickens that is an important pathogen for the poultry industry. MD is caused by MD virus (MDV) that ... More
BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity.
AuthorsShlyakhtina Y,Bloechl B,Portal MM
JournalNature communications
PubMed ID36841849
Cell plasticity is a core biological process underlying a myriad of molecular and cellular events taking place throughout organismal development and evolution. It has been postulated that cellular systems thrive to balance the organization of meta-stable states underlying this phenomenon, thereby maintaining a degree of populational homeostasis compatible with an ... More
A Novel Early Life Stress Model Affects Brain Development and Behavior in Mice.
AuthorsShin HS,Choi SM,Lee SH,Moon HJ,Jung EM
JournalInternational journal of molecular sciences
PubMed ID36902120
Early life stress (ELS) in developing children has been linked to physical and psychological sequelae in adulthood. In the present study, we investigated the effects of ELS on brain and behavioral development by establishing a novel ELS model that combined the maternal separation paradigm and mesh platform condition. We found ... More
CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes.
AuthorsHan JL,Heinson YW,Chua CJ,Liu W,Entcheva E
JournalCommunications biology
PubMed ID38062109
Uncovering gene-phenotype relationships can be enabled by precise gene modulation in human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs) and follow up phenotyping using scalable all-optical electrophysiology platforms. Such efforts towards human functional genomics can be aided by recent CRISPR-derived technologies for reversible gene inhibition or activation (CRISPRi/a). We set out to ... More
Isoflurane Anesthesia's Impact on Gene Expression Patterns of Rat Brains in an Ischemic Stroke Model.
Background: Ischemic stroke (IS) is one of the most severe brain diseases. Animal models with anesthesia are actively used to study stroke genomics and pathogenesis. However, the anesthesia-related gene expression patterns of ischemic rat brains remain poorly understood. In this study, we sought to elucidate the impact of isoflurane (ISO) ... More
Discovery of New Small Molecule Hits as Hepatitis B Virus Capsid Assembly Modulators: Structure and Pharmacophore-Based Approaches.
AuthorsSenaweera S,Du H,Zhang H,Kirby KA,Tedbury PR,Xie J,Sarafianos SG,Wang Z
JournalViruses
PubMed ID33925540
Hepatitis B virus (HBV) capsid assembly modulators (CpAMs) have shown promise as potent anti-HBV agents in both preclinical and clinical studies. Herein, we report our efforts in identifying novel CpAM hits via a structure-based virtual screening against a small molecule protein-protein interaction (PPI) library, and pharmacophore-guided compound design and synthesis. ... More
Synergistic Effect of 300 μm Needle-Depth Fractional Microneedling Radiofrequency on the Treatment of Senescence-Induced Aging Hyperpigmentation of the Skin.
JournalInternational journal of molecular sciences
PubMed ID34299100
Aging-associated dermatological pigmentary diseases are associated with accumulation of senescence cells and the disruption of basement membrane due to chronic ultraviolet radiation (UVR) exposure. Our study is on the synergistic effect of the novel 300 μm needle-depth fractional microneedling radiofrequency (FMR) treatment and conventional Q-switched ND:YAG laser on aging-associated hyperpigmentation ... More
SETD7-mediated monomethylation is enriched on soluble Tau in Alzheimer's disease.
BACKGROUND: Human tauopathies including Alzheimerâs disease (AD) are characterized by alterations in the post-translational modification (PTM) pattern of Tau, which parallel the formation of insoluble Tau aggregates, neuronal dysfunction and degeneration. While PTMs on aggregated Tau have been studied in detail, much less is known about the modification patterns of ... More
Integrin β3-Mediated Cell Senescence Associates with Gut Inflammation and Intestinal Degeneration in Models of Alzheimer's Disease.
AuthorsTun X,Wang EJ,Gao Z,Lundberg K,Xu R,Hu D
JournalInternational journal of molecular sciences
PubMed ID36982771
Alzheimerâs disease (AD) is a neurodegenerative disorder characterized by memory loss and personality changes that ultimately lead to dementia. Currently, 50 million people worldwide suffer from dementia related to AD, and the pathogenesis underlying AD pathology and cognitive decline is unknown. While AD is primarily a neurological disease of the ... More
Engineered expression of the invertebrate-specific scorpion toxin AaHIT reduces adult longevity and female fecundity in the diamondback moth Plutella xylostella.
Long non‑coding RNA LINC00491 promotes proliferation and inhibits apoptosis in esophageal squamous cell carcinoma.
AuthorsYe H,Mulmi Shrestha S,Zhu J,Ding Y,Shi R
JournalInternational journal of molecular medicine
PubMed ID33537830
Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor in the human digestive system, which affects the physical and mental health of the patient. Long non-coding (lnc)RNAs have been revealed to play an important role in human malignant tumors. Moreover, long intergenic non-protein coding RNA 491 (LINC00491) is a ... More
Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis.
AuthorsMilosevic A,Lavrnja I,Savic D,Milosevic K,Skuljec J,Bjelobaba I,Janjic MM
JournalCells
PubMed ID37048118
Multiple sclerosis (MS) is an autoimmune disease affecting the CNS and occurring far more prevalently in women than in men. In both MS and its animal models, sex hormones play important immunomodulatory roles. We have previously shown that experimental autoimmune encephalomyelitis (EAE) affects the hypothalamicâpituitaryâgonadal axis in rats of both ... More
Development and Validation of the Oxidative Stress Related lncRNAs for Prognosis in Esophageal Squamous Cell Carcinoma.
AuthorsZheng X,Liu W,Zhu Y,Kong W,Su X,Huang L,Cui Y,Sun G
JournalCancers
PubMed ID37686677
SIMPLE SUMMARY: Esophageal squamous cell cancer (ESCC) is an aggressive disease associated with a poor prognosis. The oxidative stress-related long non-coding RNAs (lncRNAs) play crucial roles in tumor prognosis. Our study identified seven oxidative stress-related DElncRNAs in the ESCC and build a prognostic risk model. The model exhibited an excellent ... More
An Engineered Probiotic Platform for Cancer Epitope-Independent Targeted Radionuclide Therapy of Solid Tumors.
Targeted radionuclide therapy is an emerging therapeutic modality for the treatment of various forms of solid cancers. Current approaches rely on the presence of cancer-specific epitopes and receptors against which a radiolabeled ligand is systemically administered to specifically deliver cytotoxic doses of α and β particles to tumors. In this ... More
Transposable elements activation triggers necroptosis in mouse embryonic stem cells.
Deficiency of the histone H3K9 methyltransferase SETDB1 induces RIPK3-dependent necroptosis in mouse embryonic stem cells (mESCs). However, how necroptosis pathway is activated in this process remains elusive. Here we report that the reactivation of transposable elements (TEs) upon SETDB1 knockout is responsible for the RIPK3 regulation through both cis and ... More
TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus.
AuthorsShang S,He D,Liu C,Bao X,Han S,Wang L
JournalMicrobiology spectrum
PubMed ID38018974
Aspergillus fumigatus is an opportunistic pathogen that infects immunocompromised patients and imposes a heavy burden on global health. Searching for key genes in host resistance to A. fumigatus infection can help us understand the mechanisms of A. fumigatus-host interactions and provide new targets for the treatment of fungal infections. TRAF3 ... More
A transcriptomic signature of X chromosome overdosage in Saudi Klinefelter syndrome induced pluripotent stem cells.
OBJECTIVE: The transcriptional landscape of Klinefelter syndromeduring early embryogenesis remains elusive. This study aimed to evaluate the impact of X chromosome overdosage in 47,XXY males induced pluripotent stem cells (iPSCs) obtained from patients with different genomic backgrounds and ethnicities. DESIGN AND METHOD: We derived and characterized 15 iPSC lines from ... More
Dietary supplementation of microalgae mitigates the negative effects of heat stress in broilers.
AuthorsChaudhary A,Mishra P,Amaz SA,Mahato PL,Das R,Jha R,Mishra B
JournalPoultry science
PubMed ID37540947
Heat stress in poultry is a serious concern, affecting their health and productivity. To effectively address the issue of heat stress, it is essential to include antioxidant-rich compounds in the poultry diet to ensure the proper functioning of the redox system. Microalgae (Spirulina platensis) are rich in antioxidants and have ... More
The in-vitro effect of famotidine on sars-cov-2 proteases and virus replication.
The lack of coronavirus-specific antiviral drugs has instigated multiple drug repurposing studies to redirect previously approved medicines for the treatment of SARS-CoV-2, the coronavirus behind the ongoing COVID-19 pandemic. A recent, large-scale, retrospective clinical study showed that famotidine, when administered at a high dose to hospitalized COVID-19 patients, reduced the ... More
A small molecule BCL6 inhibitor as chemosensitizers in acute myeloid leukemia.
PolyPurine Reverse Hoogsteen Hairpins Work as RNA Species for Gene Silencing.
AuthorsAubets E,Chillon M,Ciudad CJ,Noé V
JournalInternational journal of molecular sciences
PubMed ID34576188
PolyPurine Reverse Hoogsteen Hairpins (PPRHs) are gene-silencing DNA-oligonucleotides developed in our laboratory that are formed by two antiparallel polypurine mirror repeat domains bound intramolecularly by Hoogsteen bonds. The aim of this work was to explore the feasibility of using viral vectors to deliver PPRHs as a gene therapy tool. After ... More
Chemical profiling of HIV-1 capsid-targeting antiviral PF74.
The capsid protein (CA) of HIV-1 plays essential roles in multiple steps of the viral replication cycle by assembling into functional capsid core, controlling the kinetics of uncoating and nuclear entry, and interacting with various host factors. Targeting CA represents an attractive yet underexplored antiviral approach. Of all known CA-targeting ... More
Acyl-CoA synthetase 6 is required for brain docosahexaenoic acid retention and neuroprotection during aging.
The omega-3 fatty acid docosahexaenoic acid (DHA) inversely relates to neurological impairments with aging; however, limited nondietary models manipulating brain DHA have hindered a direct linkage. We discovered that loss of long-chain acyl-CoA synthetase 6 in mice (Acsl6(â/â)) depletes brain membrane phospholipid DHA levels, independent of diet. Here, Acsl6(â/â) brains ... More
p-Aminophenylalanine Involved in the Biosynthesis of Antitumor Dnacin B1 for Quinone Moiety Formation.
Actinosynnema species produce diverse natural products with important biological activities, which represent an important resource of antibiotic discovery. Advances in genome sequencing and bioinformatics tools have accelerated the exploration of the biosynthetic gene clusters (BGCs) encoding natural products. Herein, the completed BGCs of dnacin B1 were first discovered in two ... More
Evaluating extraction methods to study canine urine microbiota.
AuthorsMrofchak R,Madden C,Evans MV,Hale VL
JournalPloS one
PubMed ID34242284
The urinary microbiota is the collection of microbes present in urine that may play a role in host health. Studies of urine microbiota have traditionally relied upon culturing methods aimed at identifying pathogens. However, recent culture-free sequencing studies of the urine microbiota have determined that a diverse array of microbes ... More
Probiotics, Proline and Calcium Induced Protective Responses of Triticum aestivum under Drought Stress.
AuthorsMockevičiūtė R,Jurkonienė S,Šveikauskas V,Zareyan M,Jankovska-Bortkevič E,Jankauskienė J,Kozeko L,Gavelienė V
JournalPlants (Basel, Switzerland)
PubMed ID36986989
In order to increase plants tolerance to drought, the idea of treating them with stress-protecting compounds exogenously is being considered. In this study, we aimed to evaluate and compare the impact of exogenous calcium, proline, and plant probiotics on the response of winter wheat to drought stress. The research was ... More
Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.
Microtubules play crucial roles in cellular architecture, intracellular transport, and mitosis. The availability of free tubulin subunits affects polymerization dynamics and microtubule function. When cells sense excess free tubulin, they trigger degradation of the encoding mRNAs, which requires recognition of the nascent polypeptide by the tubulin-specific ribosome-binding factor TTC5. How ... More
GPR110 ligands reduce chronic optic tract gliosis and visual deficit following repetitive mild traumatic brain injury in mice.
AuthorsChen H,Kevala K,Aflaki E,Marugan J,Kim HY
JournalJournal of neuroinflammation
PubMed ID34273979
BACKGROUND: Repetitive mild traumatic brain injury (mTBI) can result in chronic visual dysfunction. G-protein receptor 110 (GPR110, ADGRF1) is the target receptor of N-docosahexaenoylethanolamine (synaptamide) mediating the anti-neuroinflammatory function of synaptamide. In this study, we evaluated the effect of an endogenous and a synthetic ligand of GPR110, synaptamide and (4Z,7Z,10Z,13Z,16Z,19Z)-N-(2-hydroxy-2-methylpropyl) ... More
Nanocurcumin Reduces High Glucose and Particulate Matter-Induced Endothelial Inflammation: Mitochondrial Function and Involvement of miR-221/222.
PURPOSE: Particulate matter (PM) 2.5, harmful air pollutants, and diabetes are associated with high morbidity and mortality from cardiovascular disease (CVD). However, the molecular mechanisms underlying the combined effects of PM and diabetes on CVD remain unclear. METHODS: Endothelial cells (ECs) treated with high glucose (HG) and PM mimic hyperglycemia ... More
Multi-modulation of immune-inflammatory response using bioactive molecule-integrated PLGA composite for spinal fusion.
Despite current developments in bone substitute technology for spinal fusion, there is a lack of adequate materials for bone regeneration in clinical applications. Recombinant human bone morphogenetic protein-2 (rhBMP-2) is commercially available, but a severe inflammatory response is a known side effect. Bone graft substitutes that enhance osteogenesis without adverse ... More
HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype.
Appropriately manipulating macrophage M1/M2 phenotypic transition is a promising therapeutic strategy for tissue repair after myocardial infarction (MI). Here we showed that gene ablation of hypoxia-induced mitogenic factor (HIMF) in mice (Himf(â/â) and HIMF(flox/flox);Lyz2-Cre) attenuated M1 macrophage-dominated inflammatory response and promoted M2 macrophage accumulation in infarcted hearts. This in turn ... More
let-7g counteracts endothelial dysfunction and ameliorating neurological functions in mouse ischemia/reperfusion stroke model.
AuthorsBernstein DL,Gajghate S,Reichenbach NL,Winfield M,Persidsky Y,Heldt NA,Rom S
JournalBrain, behavior, and immunity
PubMed ID32017988
Stroke is a debilitating disease, accounting for almost 20% of all hospital visits, and 8% of all fatalities in the United States in 2017. Following an ischemic attack, inflammatory processes originating from endothelial cells within the brain microvasculature can induce many toxic effects into the impacted area, from both sides ... More
Expression analysis and targets prediction of microRNAs in OGD/R treated astrocyte-derived exosomes by smallRNA sequencing.
Fat mass and obesity-associated (FTO) gene is essential for insulin secretion and β-cell function: In vitro studies using INS-1 cells and human pancreatic islets.
The Multikinase Inhibitor AD80 Induces Mitotic Catastrophe and Autophagy in Pancreatic Cancer Cells.
AuthorsLima K,de Miranda LBL,Del Milagro Bernabe Garnique A,de Almeida BO,do Nascimento MC,Alcântara GAS,Machado-Santelli GM,Rego EM,Machado-Neto JA
JournalCancers
PubMed ID37568682
SIMPLE SUMMARY: Pancreatic cancer is one of the most lethal human neoplasms, and its therapeutic repertoire remains limited. Advances in understanding the molecular complexity involved in the biology of the disease have paved the way for new therapeutic opportunities. AD80 is a multikinase inhibitor that inhibits S6K as well as ... More
EGCG-induced selective death of cancer cells through autophagy-dependent regulation of the p62-mediated antioxidant survival pathway.
JournalMolecular therapy : the journal of the American Society of Gene Therapy
PubMed ID33068777
Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant disorder characterized by a progressive, asymmetric weakening of muscles, starting with those in the upper body. It is caused by aberrant expression of the double homeobox protein 4 gene (DUX4) in skeletal muscle. FSHD is currently incurable. We propose to develop a ... More
LCRMP-1 is required for spermatogenesis and stabilises spermatid F-actin organization via the PI3K-Akt pathway.
Long-form collapsin response mediator protein-1 (LCRMP-1) belongs to the CRMP family which comprises brain-enriched proteins responsible for axon guidance. However, its role in spermatogenesis remains unclear. Here we find that LCRMP-1 is abundantly expressed in the testis. To characterize its physiological function, we generate LCRMP-1-deficient mice (Lcrmp-1(â/â)). These mice exhibit ... More