Attune™ NxT Flow Cytometer, blue/red/violet6 - FAQs

View additional product information for Attune™ NxT Flow Cytometer, blue/red/violet6 - FAQs (A29003CFR)

8 product FAQs found

I'd like to use the Flow Cytometry Panel Builder but will be purchasing antibodies from a different vendor than Thermo Fisher Scientific. Is this possible?

Step 2 allows for antibodies you have already purchased or are from a vendor outside Thermo Fisher Scientific. If you are undecided about which format you want for something, you can add the antigen name in the "Antibodies you need" section and then choose a placeholder fluorochrome in the following step. Both will help you keep track of channels that are reserved for reagents that you do not plan to buy from Thermo Fisher Scientific.

Can I export the spreadsheet from the Flow Cytometry Panel Builder for later use?

There are both PDF (printable) and CSV (spreadsheet) exports available in the summary step for you to take away for reference and ordering purposes.

In the Flow Cytometry Panel Builder tool, I can't find my flow cytometer in the dropdown. Can I build one?

Yes, you can define a new cytometer using the "Enter your cytometer manually" link below the cytometer dropdown menu.

I would like to use the Flow Cytometry Panel Builder but my flow cytometer does not use a standard configuration. How can I make changes to one in the dropdown?

Select a cytometer from the dropdown menu and then select the "Edit cytometer settings" link to modify the configuration as needed.

In the Flow Cytometry Panel Builder tool, what do the colorful dots indicate in the "Fluorochromes" step?

Each dot indicates a different fluorochrome that Thermo Fisher Scientific has available in each channel of the cytometer you have chosen.

How do I share my panel generated using the Flow Cytometry Panel Builder with my lab manager?

You may export the spreadsheet found on step 5 and share it.

In the Flow Cytometry Panel Builder, my marker isn't showing up under step 2 of antigen, but I see it in the catalog. How can I remedy this?

Select your target species first, and then type your antigen. A drop-down list will appear. Select the antigen from the list and click on "Confirm selections." If you have an antibody that is not on the list, in the "Antibodies you already have" section, you can enter a name and select "use this name anyway."

In the Flow Cytometry Panel Builder tool, can I go back a step without losing the work I did?

Yes, the previous work is saved. If you plan to leave the Panel Builder and come back later, be sure to select "Save" in the lower right corner to save your panel.