MagMAX™ Cell-Free DNA Isolation Kit - FAQs

View additional product information for MagMAX™ Cell-Free DNA Isolation Kit - FAQs (A29319)

16 product FAQs found

我可以编辑出厂自带的KingFisher Flex实验方案吗？

不能，出厂实验方案不能编辑，但是您可以使用“另存为”功能对其重命名，然后将它作为一个新的版本来编辑。

我可以在KingFisher Flex仪器上使用其它厂家生产的磁珠吗？

对于核酸提取，我们建议使用我们的MagMAX试剂盒和MagJET试剂盒。其它大小在0.5到10 µm间的磁珠应该也可以使用。但是，您需要自己用Thermo Scientific BindIt软件编写您自己的KingFisher实验方案，并自己验证/优化这一实验方案。

我想使用KingFisher Flex仪器从大体积的液体样本中提取核酸。我可以使用比实验方案中建议的体积更多的样本吗？

可以，可将样品分装。在进行下一步之前，设置程序使得磁头可以从多个孔内收集样本。确保不要过量加样。

使用KingFisher Flex仪器时我可以扩大我的反应体积吗？

可以，反应体积可以扩大，并且所有其它体积（例如洗涤体积）需要以相同倍数扩大。除非板的容积允许，我们强烈建议反应体积扩大不应超过2倍。

所有Applied Biosystems MagMAX试剂盒都能用于Thermo Scientific KingFisher Flex 磁珠处理仪吗？

是的，所有MagMAX试剂盒都能用于KingFisher Flex 磁珠处理仪。

How many samples can I isolate with MagMax Cell-Free DNA Isolation Kit (Cat. No. A29319)?

The number of samples that can be isolated with MagMax Cell-Free DNA Isolation Kit (Cat. No. A29319) depends on the sample volume. The MagMAX Cell-Free DNA Isolation Kit provides a wide sample volume input range from 500 µL to 10 mL of plasma/serum or urine. For 2 mL samples, approximately 50 isolations can be carried out.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can the MagMAX Cell-Free DNA Magnetic Beads within the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319) be stored at room temperature?

If unopened, the MagMAX Cell-Free DNA Magnetic Beads within the MagMAX Cell-Free DNA Isolation Kit will be stable at room temperature for several weeks. However, for optimal performance, we recommend storing them 2-8 degrees C.

Does the MagMAX Cell-Free DNA Isolation Kit contain MagMAX magnetic beads?

No, the MagMAX Cell-Free DNA Isolation Kit contains the Dynabeads MyOne SILANE magnetic beads. For this reason we recommend using the DynaMag magnets (Cat. Nos. 12302D, 12321D).

What sample types can the MagMAX Cell-Free DNA Isolation Kit be used for?

The MagMAX Cell-Free DNA Isolation Kit has been designed to isolate cell-free DNA (cfDNA) from plasma, serum, and urine samples.

We are getting a lot of variation in cell-free DNA yield from donor to donor when using the MagMAX Cell-Free DNA Isolation Kit. Do you have any tips for how to improve that?

Levels of cell-free DNA in circulation can range from 1 ng/mL to 100 ng/mL of plasma or serum depending on the donor. For samples containing low levels of cell-free DNA, we recommend increasing the starting sample volume.

There is an abundance of gDNA in the eluate I obtained from the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319). Do you have any recommendations to prevent that from happening?

Yields from hemolytic plasma, lipemic plasma, or other compromised samples types vary greatly from donor to donor. We recommend processing these types of samples using the manual protocol.

Can I edit the manufactured KingFisher Flex protocols?

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.

Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I am working with liquid samples with high volume and want to extract nucleic acid from them using the KingFisher Flex instrument. Can I use more volume than the protocol advises?

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I scale up my reaction volume with the KingFisher Flex instrument?

Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.

Are all of the Applied Biosystems MagMAX kits validated for the KingFisher Flex Magnetic Particle Processor?

Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.