HepExtend™ Media Kit - FAQs

Gibco HepExtend培养基试剂盒包含了配制培养肝细胞所需的完全培养基的所有成份如下：

- Gibco HepExtend添加剂（货号A2737501）
- Williams E培养基，500mL（货号A1217601）
- 肝细胞维持添加剂包（货号CM4000）

HepExtend Supplement (50X) is an enriched supplement designed to enhance the culture lifespan (cell viability, function, and number of days in culture) of cryopreserved primary hepatocytes. We recommend using the supplement in conjunction with William's E Medium (Cat. No. A1217601) and Hepatocyte Maintenance Supplement Pack (Cat. No. CM4000), following the recommended hepatocyte culture methods. When cultured in the presence of the HepExtend Supplement, primary human hepatocyte lots will live and be functional for 10 days or longer depending on the lot of cells and other assay conditions. HepExtend Supplement does not contain growth factors, cytokines, fetal bovine serum, or pharmaceutical small molecules that are known to interfere with primary hepatocyte function.

The HepExtend Media Kit contains the following components needed to make the complete medium for hepatocyte culture:

- HepExtend Supplement (50X) (Cat. No.A2737501)
- Williams E Medium (500 mL) (Cat. No.A1217601)
- Primary Hepatocyte Maintenance Supplements (Cat. No.CM4000)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.