人多巴胺能神经元免疫细胞化学试剂盒
人多巴胺能神经元免疫细胞化学试剂盒
Invitrogen™

人多巴胺能神经元免疫细胞化学试剂盒

The Human Dopaminergic Neuron Immunocytochemistry Kit enables optimal image-based analysis of three key markers of neural differentiation: OTX2, FoxA2, and了解更多信息
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货号数量
A2951540 reactions
货号 A29515
价格(CNY)
11,017.00
Each
添加至购物车
数量:
40 reactions
价格(CNY)
11,017.00
Each
添加至购物车
The Human Dopaminergic Neuron Immunocytochemistry Kit enables optimal image-based analysis of three key markers of neural differentiation: OTX2, FoxA2, and tyrosine hydroxylase (TH). This high-performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment.

The Human Dopaminergic Neuron Immunocytochemistry Kit enables you to:
• Confirm expression of key cellular markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm expression of key cellular markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of floor plate progenitors and dopaminergic neurons. OTX2 and FoxA2 are critical nuclear markers while TH is an important cytoplasmic marker.

Get more information per sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OTX2 and FoxA2, and FoxA2 and TH) along with nuclear DNA staining to help save time and precious sample.

Generate beautiful multiplexed images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of key neural differentiation marker expression using commonly available blue, green, and orange/red filter sets.

Minimize unnecessary wash steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols, helping to save time and decrease the likelihood of cell loss during the staining protocol. The only washes required are after incubation with primary and secondary antibodies.

Process samples with confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

仅供科研使用。不可用于诊断程序。
规格
产品类型免疫细胞化学试剂盒
数量40 reactions
运输条件干冰
Unit SizeEach
内容与储存
  • 10 μL 山羊抗人 OT x 2 (2000 X)
  • 10 μL 小鼠抗人 FoxA2 (1000 X)
  • 10 μL 兔抗人酪氨酸羟化酶 (1000 X)
  • 20 μL Alexa Fluor 488 驴抗山羊 IgG (250 X)
    • 20 μL Alexa Fluor 488 驴抗兔 IgG (250 X)
    • 20 μL Alexa Fluor 555 驴抗小鼠 (250 X)
    • 20 μL Alexa Fluor 594 驴抗小鼠 (250 X)
    • 1小瓶 NucBlue 固定细胞 ReadyProbes 试剂 (1 X)
    • 10 mL 固定液 (1 X)
    • 10 mL 透化溶液 (1 X)
    • 20 mL 封闭溶液 (1 X)
    • 20 mL 洗涤缓冲液 (10 X)

      在 -20°C 至 +4°C 下储存

常见问题解答 (FAQ)

The Human Dopaminergic Neuron Immunocytochemistry Kit provides the NucBlue Fixed Cell Stain. If I don't have the correct filter set for this stain, can I use another nucleic acid stain?

If you use Alexa Fluor 488/Alexa Fluor 555 dye-conjugated secondary antibodies and you have a Cy5/Alexa Fluor 647 filter set, you may use a far-red nucleic acid stain, such as NucRed Dead 647 ReadyProbes Reagent (Cat. No. R37113) or SYTOX Deep Red Nucleic Acid Stain (Cat. No. S11380). Do not use these far-red nucleic acid stains if using an Alexa Fluor 594 dye-conjugated secondary antibody as there is too much spectral overlap of Alexa Fluor 594 into the Cy5/Alexa Fluor 647 range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the Human Dopaminergic Neuron Immunocytochemistry Kit for flow cytometry analysis?

We do not recommend using the Human Dopaminergic Neuron Immunocytochemistry Kit for flow cytometry analysis. The kit and protocol were specifically developed for imaging analysis (microscopy/high-content screening (HCS)).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer the individual components of the Human Dopaminergic Neuron Immunocytochemistry Kit as standalone products?

Only the NucBlue Fixed Cell ReadyProbes Reagent may be purchased separately as Cat. No. R37606. We do offer the secondary antibodies separately, but not in the same proprietary concentration as provided in the kit. We also offer formaldehyde, DPBS, BSA, and saponin as separate catalog numbers, but not in the volumes or as formulated in the kit. The primary antibodies are not available as separate stock items; all non-stock items in the kit may be available as a custom order. Please contact your Account Representative.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.