PureLink™ 微生物组 DNA 纯化试剂盒
PureLink™ 微生物组 DNA 纯化试剂盒
Invitrogen™

PureLink™ 微生物组 DNA 纯化试剂盒

PureLink™ 微生物组 DNA 纯化试剂盒可从各种样品类型（包括粪便和土壤等具有挑战性的样品）中快速纯化高质量微生物和宿主 DNA。该试剂盒采用经验证的 PureLink 离心柱技术，以实现便稳健生产可直接用于下游了解更多信息
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货号数量
A2979050 Preps kit
货号 A29790
价格（CNY)
2,851.00
飞享价
Ends: 31-Dec-2025
3,355.00
共减 504.00 (15%)
Each
添加至购物车
数量:
50 Preps kit
价格（CNY)
2,851.00
飞享价
Ends: 31-Dec-2025
3,355.00
共减 504.00 (15%)
Each
添加至购物车
PureLink™ 微生物组 DNA 纯化试剂盒可从各种样品类型（包括粪便和土壤等具有挑战性的样品）中快速纯化高质量微生物和宿主 DNA。该试剂盒采用经验证的 PureLink 离心柱技术，以实现便稳健生产可直接用于下游 PCR、测序或其他应用的纯化 DNA。高效三重裂解方法、快速去除抑制剂和多功能性使该试剂盒成为微生物组研究项目以及旨在快速检测各种样品中致病性细菌的项目的终极试剂盒。

PureLink 微生物组 DNA 纯化试剂盒的产品特点包括：

•通过利用专用微珠进行热、化学及机械破碎的组合有效裂解所有微生物（包括具有较厚和较复杂细胞壁的耐受性物种）
• 使用新型纯化缓冲液通过沉淀消除抑制剂化合物
• 适合许多生物样品的简化方案
• 高纯 DNA 回收与常见的下游应用（如 qPCR 和下一代测序）兼容

从不同样品类型组中分离微生物和宿主 DNA 的一套试剂盒
PureLink 微生物组 DNA 纯化试剂盒最大限度地减少了针对特定样品类型订购单个试剂盒的需求，因为该试剂盒经过优化可与多种生物样品配合使用。

该试剂盒可从以下样品中进行微生物—和（如适用）宿主—DNA 纯化：

•粪便
•尿液
•唾液
•拭子（阴道、口腔、皮肤、直肠、环境）
•转运培养基
•生长培养基
•土壤

分析人类微生物组
人类体内有 100 万亿细菌、古菌、真菌、原生生物和病毒，这些对我们的健康状况有重要影响。术语“微生物组”是指这些群落中的各种不同微生物。目前，它们的基因是识别这些生物体的最直接方法。健康微生物组成的偏差与许多人类疾病相关、包括炎症性肠病、肥胖、癌症、哮喘、糖尿病和过敏。

生物样品中聚合微生物 DNA 的纯化是了解微生物组如何影响人类健康的关键步骤。回收的 DNA 准确反映了样品界中多种微生物的表达，这一点至关重要。PureLink 微生物组 DNA 试剂盒中使用的机械破碎和三重裂解方法可帮助确保可从最棘手的样品（如在粪便中发现的革兰氏阳性菌）中回收微生物 DNA—以识别存在的全部微生物。
仅供研究使用。不可用于诊断程序。
规格
色谱柱类型离心柱
洗脱体积50 μL（离心）、100 μL（真空）、150 μL
最终产品类型Microbial and host DNA
适用于（应用）实时荧光定量 PCR (qPCR)、克隆、Southern 印迹、测序、PCR
高通量能力不兼容高通量应用
数量50 Preps kit
样品类型细菌, 口腔样品, 细胞, 食品和环境, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), 细胞, Food and Environmental
规格小型
运输条件室温
原始材料量Buccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab
Microbial Culture: ≤5 mL
Saliva: ≤2 mL
Soil: ≤2 g
Stool: ≤2 g
Urine: ≤10 mL
测试时间Up to 55 min. (Including sample homogenization)
产量≤25 μg
分离技术离心柱
Unit SizeEach
内容与储存
• S1 裂解缓冲液，1 x 40 mL
• S2 裂解增强剂，1 x 5 mL
• S3 纯化缓冲液，1 x 12.5 mL
• S4 结合缓冲液，1 x 45 mL
• S5 洗涤缓冲液浓缩液，1 x 13 mL
• S6 洗脱缓冲液，1 x 5 mL
• PureLink 离心柱（含收集管），50 柱/管
• PureLink 收集管，100 管
•微珠管*，50 个含有专用微珠的管
*在单独的盒子中运输。

在室温下储存。

常见问题解答 (FAQ)

I am using the PureLink Microbiome DNA Purification Kit (Cat. No. A29790). When should I remove the swab?

The swab should be removed after incubation, prior to bead beating.

Does the elution buffer in the PureLink Microbiome DNA Purification Kit (Cat. No. A29790) contain EDTA?

Yes, the elution buffer in the PureLink Microbiome DNA Purification Kit contains EDTA. An alternative that does not contain EDTA is Tris Buffer (10 mM Tris-HCl, pH 8.0-9.0). Eluting in water is possible, but the low pH of water could lead to reduced stability and less accurate OD readings.

When using the PureLink Microbiome DNA Purification Kit with soil samples, I am getting less than 400 µL supernatant after lysis. Can you provide me with any suggestions?

For some samples, including soil and stool samples, it can be difficult to withdraw 400 µL supernatant while avoiding debris. If less than 250 µL of supernatant is transferred, add S1-Lysis buffer to bring the volume to 400 µL, before adding S3-Cleanup Buffer.

I am getting low yield using PureLink Microbiome DNA Purification Kit. How can I improve the yield?

Low yield could be caused by inefficient lysis and/or low levels of DNA in the sample. Please try heating samples at 95 degrees C for 5-10 minutes instead of 65 degrees C for 10 minutes after adding S2-Lysis Enhancer. Bead beat for a longer time or at a higher power setting. You can also try to perform the experiment with more starting material, but do not exceed what we specify in the protocol. However for some challenging samples, too much starting material can also result in low yield. In this case, please try less starting material and/or increase the volume of S1-Lysis Buffer.

There are inhibitors in the isolated DNA from environmental samples using PureLink Microbiome DNA Purification Kit. Is there a way to rescue it and obtain good PCR results?

There are several options to rescue your DNA obtain good PCR results including diluting your DNA sample, using specific enzymes, and/or using an additive. The methods are described below:

- Dilute your DNA sample 10-100 times prior to PCR which will dilute out the contaminants while the PCR signal in many cases will remain strong.
- Use “tough” enzymes that were specifically designed and optimized to be effective in harsh environments, and work in the presence of high levels of inhibitors, for instance, TaqPath qPCR Master Mix, CG Reagent.
- Use a BSA additive for the PCR reaction. In most cases, if used at appropriate concentration, BSA can rescue your sample.

You can also try repeating the purification with less starting material or increased volume of S1-Lysis buffer. Additionally, after the addition of the S3- Cleanup buffer, you can try incubating the sample for 10 minutes on ice before centrifugation.

View all PureLink™ 微生物组 DNA 纯化试剂盒 FAQs