Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.
AuthorsGupta R,Kus B,Fladd C,Wasmuth J,Tonikian R,Sidhu S,Krogan NJ,Parkinson J,Rotin D
JournalMolecular systems biology
PubMed ID17551511
Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protein microarray platform that can be utilized for the ... More
Mouse marginal zone B cells harbor specificities similar to human broadly neutralizing HIV antibodies.
'A series of potent, broadly neutralizing HIV antibodies have been isolated from B cells of HIV-infected individuals. VRC01 represents a subset of these antibodies that mediate neutralization with a restricted set of IGHV genes. The memory B cells expressing these antibodies were isolated years after infection; thus, the B-cell subpopulation ... More
Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.
AuthorsRoether J, Bertels S, Oelschlaeger C, Bastmeyer M, Willenbacher N
JournalPLoS One
PubMed ID30566463
'As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required. We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells. Structure and elasticity of macroporous, three-dimensional cryogel ... More
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing.
AuthorsTabdanov ED, Puram V, Zhovmer A, Provenzano PP
JournalCell Rep
PubMed ID30304674
Cancer cell migration through and away from tumors is driven in part by migration along aligned extracellular matrix, a process known as contact guidance (CG). To concurrently study the influence of architectural and mechanical regulators of CG sensing, we developed a set of CG platforms. Using flat and nanotextured substrates ... More