Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)
Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)
Invitrogen™

Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)

The SDP (sulfodicholorphenol) ester is currently the most hydrolytically stable amine-reactive moiety available from Invitrogen. Conjugates produced with the AlexaRead more
Have Questions?
Catalog NumberQuantity
A300521 mg
Catalog number A30052
Price (CNY)
6,260.00
Each
Add to cart
Quantity:
1 mg
Price (CNY)
6,260.00
Each
Add to cart
The SDP (sulfodicholorphenol) ester is currently the most hydrolytically stable amine-reactive moiety available from Invitrogen. Conjugates produced with the Alexa Fluor™ 488 5-SDP ester produce the same strong amide bond between the dye and the compound of interest as succinimidyl (SE or NHS) and tetrafluorophenyl (TFP) esters, but with the improved stability in water and buffers, the SDP ester can potentially offer increased control and consistency in these reactions compared to the NHS and TFP esters.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 488
Product TypeSulfodichlorophenol Ester
Quantity1 mg
Reactive MoietyActive Ester, SDP Ester
Shipping ConditionRoom Temperature
Label TypeAlexa Fluor
Product LineAlexa Fluor™
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.