Integrin nanoclusters can bridge thin matrix fibres to form cell-matrix adhesions.
AuthorsChangede R, Cai H, Wind SJ, Sheetz MP
JournalNat Mater
PubMed ID31477904
'Integrin-mediated cell-matrix adhesions are key to sensing the geometry and rigidity of extracellular environments and influence vital cellular processes. In vivo, the extracellular matrix is composed of fibrous arrays. To understand the fibre geometries that are required for adhesion formation, we patterned nanolines of various line widths and arrangements in ... More
Centriole Number and the Accumulation of Microtubules Modulate the Timing of Apical Insertion during Radial Intercalation.
AuthorsCollins C, Majekodunmi A, Mitchell B
JournalCurr Biol
PubMed ID32243862
'Centrioles are microtubule (MT)-based structures that provide important functions during cell migration, cell division, and cell signaling [1]. Modulating centriole number in 3D cell cultures has been shown to influence protrusive behavior [2-5]. Here, we address in vivo the role of centrioles and the accumulation of MTs on the protrusive behavior ... More
Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.
AuthorsPauwels AM, Härtlova A, Peltier J, Driege Y, Baudelet G, Brodin P, Trost M, Beyaert R, Hoffmann E,
JournalMol Cell Proteomics
PubMed ID30808727
'Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes. These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune ... More
TMIE Defines Pore and Gating Properties of the Mechanotransduction Channel of Mammalian Cochlear Hair Cells.
AuthorsCunningham CL, Qiu X, Wu Z, Zhao B, Peng G, Kim YH, Lauer A, Müller U
JournalNeuron
PubMed ID32343945
'TMC1 and TMC2 (TMC1/2) have been proposed to form the pore of the mechanotransduction channel of cochlear hair cells. Here, we show that TMC1/2 cannot form mechanotransduction channels in cochlear hair cells without TMIE. TMIE binds to TMC1/2, and a TMIE mutation that perturbs TMC1/2 binding abolishes mechanotransduction. N-terminal TMIE ... More
Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis.
AuthorsCollins KB, Kang H, Matsche J, Klomp JE, Rehman J, Malik AB, Karginov AV
JournalJ Cell Biol
PubMed ID31865373
Podosomes are compartmentalized actin-rich adhesions, defined by their ability to locally secrete proteases and remodel extracellular matrix. Matrix remodeling by endothelial podosomes facilitates invasion and thereby vessel formation. However, the mechanisms underlying endothelial podosome formation and function remain unclear. Here, we demonstrate that Septin2, Septin6, and Septin7 are required for ... More