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查看更多产品信息 Phalloidin Labeling Probes - FAQs (R415, A12379, A30104, B7474, A22282, A30105, A22281, A30106, A30107, A22286, P3457, F432, A22285, A22284, A22283, T7471, A22287, B3475, A34055, A12380, O7466, A12381, A34054)
3 个常见问题解答
We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.
For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.
Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
No. They differ only in the dyes attached to the phalloidin. The phalloidin has the same structure in all of the products.
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No. The dye in Alexa Fluor Plus 750 Phalloidin is structurally different from Alexa Fluor 750 dye and has distinct spectral properties; Alexa Fluor 750 dye has excitation/emission maxima of 753/782 nm; Alexa Fluor Plus 750 Phalloidin has excitation/emission maxima of 758/784 nm.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.