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Invitrogen™
Phalloidin Labeling Probes
Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
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| 货号 | 颜色 | 激发波长范围 | 染料类型 |
|---|---|---|---|
| A30106 | Orange | 555/565 nm | Alexa Fluor Plus 555 |
| A22281 | Blue | 346⁄442 | Alexa Fluor™ 350 |
| A30104 | Violet | 405/450 | Alexa Fluor™ Plus 405 |
| A12379 | Green | 激发波长495 nm 发射波长518 nm | Alexa Fluor™ 488 |
| O7466 | Green | 496⁄520 | Oregon Green™ 488 |
| F432 | Green | 496⁄516 | FITC(荧光素) |
| A22282 | Yellow | 531⁄554 | Alexa Fluor™ 532 |
| R415 | Red-orange | 540⁄565 | TRITC(四甲基异硫氰酸罗达明) |
| A22283 | Orange | 556⁄570 | Alexa Fluor™ 546 |
| A34055 | Orange | 555⁄565 | Alexa Fluor™ 555 |
| B3475 | Red | 558⁄569 | BODIPY™ |
| A12380 | Orange-red | 578⁄600 | Alexa Fluor™ 568 |
| A12381 | Red | 581⁄609 | Alexa Fluor™ 594 |
| T7471 | Red | 591⁄608 | Texas Red™ |
| A22284 | Far-red | 632⁄647 | Alexa Fluor™ 633 |
| A34054 | Far-red | 633⁄647 | Alexa Fluor™ 635 |
| A22287 | Far-red | 650⁄668 | Alexa Fluor™ 647 |
| A30107 | Far-red | 650/668 nm | Alexa Fluor Plus 647 |
| A22285 | Near-infrared | 663⁄690 | Alexa Fluor™ 660 |
| A22286 | Near-infrared | 679⁄702 | Alexa Fluor™ 680 |
| A30105 | Near-infrared | 758/784 | Alexa Fluor™ Plus 750 |
货号 A30106
价格（CNY)
5,057.00
飞享价
Ends: 31-Dec-2025
6,854.00共减 1,797.00 (26%)
Each
颜色:
Orange
激发波长范围:
555/565 nm
染料类型:
Alexa Fluor Plus 555
价格（CNY)
5,057.00
飞享价
Ends: 31-Dec-2025
6,854.00共减 1,797.00 (26%)
Each
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.
A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.
Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.
Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining
Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.
Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.
Features of phalloidin probes
- High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
- Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
- Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
- Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
- Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
- Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
- Ease of use—staining is straightforward and quick
- Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
- Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton
仅供科研使用。不可用于诊断程序。
规格
颜色Orange
染料类型Alexa Fluor Plus 555
激发波长范围555/565 nm
适用于（设备）Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with TRITC/RFP filter set
产品线Alexa Fluor™
数量1 Ea.
运输条件经检验可环境温度或湿冰运输
标签类型Alexa Fluor 染料
产品类型鬼笔环肽
亚细胞定位肌动蛋白Actin
Unit SizeEach
内容与储存
含有1个含足量材料的样品瓶,适用于300单位/玻片。
收货后,在 ≤–20°C 下储存。干燥避光保存。
按照指示条件储存,产品可保持稳定至少1年。
收货后,在 ≤–20°C 下储存。干燥避光保存。
按照指示条件储存,产品可保持稳定至少1年。