7500 Fast 仪器包(带笔记本电脑)
7500 Fast 仪器包(带笔记本电脑)
Applied Biosystems™

7500 Fast 仪器包(带笔记本电脑)

Meet your pathogen and authenticity testing needs with the Applied Biosystems 7500 Fast Food Safety Real-Time PCR System. This is了解更多信息
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货号描述
A302997500Fast(带便携式计算机)
货号 A30299
价格(CNY)
-
申请报价
描述:
7500Fast(带便携式计算机)
Meet your pathogen and authenticity testing needs with the Applied Biosystems 7500 Fast Food Safety Real-Time PCR System. This is an integrated and versatile real-time PCR system that combines thermal cycling, fluorescence detection, and application-specific software to measure the cycle-by-cycle accumulation of PCR products in a single-tube homogeneous reaction.

The 7500 Fast Food Safety Real-Time PCR System is compatible with all Thermo Fisher Scientific food safety PCR assays. These assays offer detection of the major pathogens and other important targets in food samples.

Expand your in-house PCR capability beyond day-to-day pathogen testing with the 7500 Fast Real-Time PCR System—a versatile, 96-well platform that provides flexibility to tackle your most challenging test requirements.

Flexible—five-color detection system allows a variety of applications including gene expression analysis, SNP genotyping, and plus/minus assays that utilize internal positive controls
Efficient—facilitates processing of multiple assays in the same run
Convenient 96-well format—minimizes the effort required to perform sample setup and allows the use of tube strips
Simple intuitive software—enables rapid, straightforward PCR set-up, automatic data interpretation, and traceability of results
Small instrument footprint—fits easily into laboratory environments where bench space is at a premium
Instrument service and support—instruments are fully supported by our own service engineers and dedicated, knowledgeable technical team

The 7500 Fast Real-time PCR System is compatible with our SureTect Real-Time PCR Food Pathogen Assays; MicroSEQ, RapidFinder, and TaqMan assays; and RapidFinder Salmonella species, Typhimurium, and Enteritidis Multiplex Assay.

The 7500 Fast Real-Time PCR System delivers high-speed cycling capabilities with a new fast-ramping Peltier-based thermal cycling block. The instrument, Optical Fast 96-well reaction plates, and hot-start DNA polymerase containing master mix comprise the complete Fast system. With this system, quantitative real-time PCR runs can be completed in about 50 minutes with comparable performance to standard run times for supported applications.

The advanced optical configuration supports a broader range of fluorophores, including FAM/SYBR Green I, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 dyes; variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

仅供食品和环境样本检验使用。
For Testing of Food and Environmental Samples Only.
规格
深度(英制)22 in.
深度(公制)56.5 cm
描述7500Fast(带便携式计算机)
显示类型8.4 英寸彩色 TFT LCD
适用于(应用)实时 PCR (qPCR)
适用于(设备)7500 Fast 仪器包
产品规格96 孔板
高度(英制)10.6 in.
高度(公制)27.2 cm
高通量能力兼容高通量应用
最大变温速率3°C/s(模块),1.6°C/s(样品)
数量1 system
反应速度快速
运输条件室温
热量准确度±0.0.25°C(35°C 至 99.9°C)
热量范围0°C 至 100°C
热量均匀性<0.5°C(达到 95°C 后20秒)
用户界面以太网
电压100 至 240 V
重量20.4 kg(45 lb.)
宽度(英制)13 in.
宽度(公制)33 cm
模块配置1 × 96 孔板
容量12 x 8联排管、1 x 96孔板 (Fast)
产品类型PCR仪(热循环仪,基因扩增仪)
Unit SizeEach
套装组件
7500 Fast Real-Time PCR System, laptop, QST
货号
4365464
单位规格
1 system
数量
1
RapidFinder™ Express Software v2.0 CD Kit
货号
A28811
单位规格
1 kit
数量
1
MicroAmp™ Cap Installing Tool
货号
4330015
单位规格
1 tool
数量
1
MicroAmp™ Optical 8-Cap Strips
货号
4323032
单位规格
300 strips
数量
1
MicroAmp™ Fast 8-Tube Strip, 0.1 mL
货号
4358293
单位规格
125 strips
数量
1
内容与储存
包括一台实时 PCR 仪器、一台戴尔便携式计算机、RapidFinder Express v2.0 软件和 RNase 板。

常见问题解答 (FAQ)

My 7500 Fast Real-Time PCR System has been upgraded to v2.0.6, so why can't I analyze my files with HRM Software v2.0.1?

For the 7500 Fast Real-Time PCR System, if the software version is below v2.0.4, the compatible HRM Software is v2.0.1. If the 7500 Fast Real-Time PCR System has been upgraded to software version 2.0.4 or above, then you will need to get HRM Software version 3.0.1.

How do I perform the HRM calibration on my real-time PCR instrument?

The HRM calibration is a 3-step procedure. In some instrument software, the calibration process has been streamlined to a single-step procedure (applies to the ViiA7 Real-Time PCR System and QuantStudio Real-Time PCR Systems).

For the HRM calibration, the first step is a PCR reaction to generate the HRM dye plate. The reaction plate contains a DNA template, a primer pair, and the PCR mix with HRM dye. The second step is dye calibration using the PCR plate from step 1. The last step is a melt curve run, again using the same plate. For details, please refer to the HRM guides for each instrument:

- 7500 Fast Real-Time PCR System (with software version 1.4 or 2.0.4 and below) (https://tools.thermofisher.com/content/sfs/manuals/cms_050347.pdf)
- 7500 Fast Real-Time PCR System (with software version 2.0.5 and above) (https://tools.thermofisher.com/content/sfs/manuals/4457847.pdf)
- 7900HT Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/cms_050347.pdf)
- StepOne and StepOnePlus Real-Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/4457847.pdf)
- ViiA 7 Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/4443531.pdf)
- QuantStudio 6 and 7 Real-Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/4489823.pdf)
- QuantStudio 6 and 7 Pro Real-Time PCR Systems (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018981_HighResolutionMelt_DA_SW_UG.pdf)
- QuantStudio 3 and 5 Real Time PCR Systems (https://tools.thermofisher.com/content/sfs/manuals/MAN0014394_HighResMeltExperiment_GSG.pdf)
- QuantStudio 12K Flex Real-Time PCR System (https://tools.thermofisher.com/content/sfs/manuals/4470692A.pdf)

Find additional tips, troubleshooting help, and resources within our High Resolution Melt Support Center.

Is my real-time PCR instrument compatible with HRM?

Among our real-time PCR instruments, the following are compatible with HRM:

- 7500 Fast Real-Time PCR System
- 7900HT Fast Real-Time PCR System
- StepOne and StepOnePlus Real-Time PCR Systems
- ViiA 7 Real-Time PCR System
- QuantStudio 6 and 7 Pro Real-Time PCR Systems
- QuantStudio 6 and 7 Flex Real-Time PCR Systems
- QuantStudio 12K Flex Real-Time PCR System
- QuantStudio 1 Real-Time PCR System
- QuantStudio 3 Real-Time PCR System
- QuantStudio 5 Real-Time PCR System

Why are there no RQ values for all the samples in my study from the 7500 or 7500 Fast Real-Time PCR System software?

The 7500 software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have CT values for all the wells, but the software will not calculate RQ values.

If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist software instead. This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7500 software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Why do I not see any CT or RQ values from my ddCT experiment in the software for the 7500 or 7500 Fast Real-Time PCR System?

If you have the SDS v1.4 or earlier, you will need to open the dd CT plate in a Study first in order to see the CT or RQ values. (If you have v2.0.x this error does not apply.)

1.After the initial run is complete, open, review the plots and save it. Then:
-Choose File then New
-Choose: Assay then ddCT (Relative Quantitation) Study. Set the other parameters as appropriate and click Next.
2.Click on ‘Add Plates...', then browse to where your data file is located and select the plate (or multiple plates).
Click ‘Finish', and you should see your study.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.