Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.
AuthorsLee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,
JournalClin Cancer Res
PubMed ID18559604
'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More
Dynamic dual-tracer MRI-guided fluorescence tomography to quantify receptor density in vivo.
AuthorsDavis SC, Samkoe KS, Tichauer KM, Sexton KJ, Gunn JR, Deharvengt SJ, Hasan T, Pogue BW,
Journal
PubMed ID23671066
The up-regulation of cell surface receptors has become a central focus in personalized cancer treatment; however, because of the complex nature of contrast agent pharmacokinetics in tumor tissue, methods to quantify receptor binding in vivo remain elusive. Here, we present a dual-tracer optical technique for noninvasive estimation of specific receptor ... More
Chemically modified firefly luciferase is an efficient source of near-infrared light.
AuthorsBranchini BR, Ablamsky DM, Rosenberg JC,
JournalBioconjug Chem
PubMed ID20936788
Bioluminescence and bioluminescence resonance energy transfer (BRET) are two naturally occurring light emission phenomena that have been adapted to a wide variety of important research applications including in vivo imaging and enzyme assays. The luciferase enzyme from the North American firefly, which produces yellow-green light, is a key component of ... More