New aldehyde tag sequences identified by screening formylglycine generating enzymes in vitro and in vivo.
AuthorsRush JS, Bertozzi CR,
JournalJ Am Chem Soc
PubMed ID18722427
'Formylglycine generating enzyme (FGE) performs a critical posttranslational modification of type I sulfatases, converting cysteine within the motif CxPxR to the aldehyde-bearing residue formylglycine (FGly). This concise motif can be installed within heterologous proteins as a genetically encoded ' ... More
Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag.
AuthorsWu P, Shui W, Carlson BL, Hu N, Rabuka D, Lee J, Bertozzi CR,
JournalProc Natl Acad Sci U S A
PubMed ID19202059
'The properties of therapeutic proteins can be enhanced by chemical modification. Methods for site-specific protein conjugation are critical to such efforts. Here, we demonstrate that recombinant proteins expressed in mammalian cells can be site-specifically modified by using a genetically encoded aldehyde tag. We introduced the peptide sequence recognized by the ... More
Enzyme-catalyzed transfer of a ketone group from an S-adenosylmethionine analogue: a tool for the functional analysis of methyltransferases.
AuthorsLee BW, Sun HG, Zang T, Kim BJ, Alfaro JF, Zhou ZS,
JournalJ Am Chem Soc
PubMed ID20196537
'S-adenosylmethionine (AdoMet or SAM)-dependent methyltransferases belong to a large and diverse family of group-transfer enzymes that perform vital biological functions on a host of substrates. Despite the progress in genomics, structural proteomics, and computational biology, functional annotation of methyltransferases remains a challenge. Herein, we report the synthesis and activity of ... More
Introducing genetically encoded aldehydes into proteins.
AuthorsCarrico IS, Carlson BL, Bertozzi CR
JournalNat Chem Biol
PubMed ID17450134
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) ... More