Enhanced spontaneous Ca(2+) events in endothelial cells reflect signalling through myoendothelial gap junctions in pressurized mesenteric arteries.
AuthorsKansui Y, Garland CJ, Dora KA,
JournalCell Calcium
PubMed ID18191200
'Increases in global Ca(2+) in the endothelium are a crucial step in releasing relaxing factors to modulate arterial tone. In the present study we investigated spontaneous Ca(2+) events in endothelial cells, and the contribution of smooth muscle cells to these Ca(2+) events, in pressurized rat mesenteric resistance arteries. Spontaneous Ca(2+) ... More
A novel mechanism for protein delivery: granzyme B undergoes electrostatic exchange from serglycin to target cells.
AuthorsRaja SM, Metkar SS, Höning S, Wang B, Russin WA, Pipalia NH, Menaa C, Belting M, Cao X, Dressel R, Froelich CJ
JournalJ Biol Chem
PubMed ID15788411
The molecular interaction of secreted granzyme B-serglycin complexes with target cells remains undefined. Targets exposed to double-labeled granzyme B-serglycin complexes show solely the uptake of granzyme B. An in vitro model demonstrates the exchange of the granzyme from serglycin to immobilized, sulfated glycosaminoglycans. Using a combination of cell binding and ... More