Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution.
AuthorsHuang B, Jones SA, Brandenburg B, Zhuang X,
JournalNat Methods
PubMed ID19029906
The ability to directly visualize nanoscopic cellular structures and their spatial relationship in all three dimensions will greatly enhance our understanding of molecular processes in cells. Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions. To facilitate ... More
Cascade blue derivatives: water soluble, reactive, blue emission dyes evaluated as fluorescent labels and tracers.
AuthorsWhitaker JE, Haugland RP, Moore PL, Hewitt PC, Reese M, Haugland RP
JournalAnal Biochem
PubMed ID1724118
Fluorescent dyes based on the pyrenyloxytrisulfonic acid (Cascade Blue) structure were prepared and evaluated. The dyes contain functional groups that react with amines, thiols, acids, aldehydes, and ketones, forming covalently bonded, fluorescent derivatives of molecules with broad biological interest. Reactive groups in the Cascade Blue dyes include carboxylic acids and ... More