Alexa Fluor™ 488 尸胺
Alexa Fluor™ 488 尸胺
引用和文献 (8)
Invitrogen™

Alexa Fluor™ 488 尸胺

Alexa Fluor™ 488 尸胺在作为一种极性示踪剂和作为通过羧酸部分标记蛋白的反应性染料时非常有用。Alexa Fluor™ 488 是一种明亮的绿色荧光染料，其激发光谱非常适合于 488 nm了解更多信息
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货号数量
A306761 mg
货号 A30676
价格（CNY)
5,564.00
Each
添加至购物车
数量:
1 mg
价格（CNY)
5,564.00
Each
添加至购物车
Alexa Fluor™ 488 尸胺在作为一种极性示踪剂和作为通过羧酸部分标记蛋白的反应性染料时非常有用。Alexa Fluor™ 488 是一种明亮的绿色荧光染料，其激发光谱非常适合于 488 nm 激光谱线。Alexa Fluor™ 488 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性（pH 值 4 - pH 值 10）。除反应性染料配方外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 Alexa Fluor™ 488 染料（了解更多信息）。

关于该 AlexaFluor™ 尸胺的详细信息：

•荧光基团标记：Alexa Fluor™ 488 染料
•反应性基团：尸胺
•反应性：羧酸、醛和酮（以及通过酶催化酰胺基转移反应产生的谷氨酰胺残基）
•偶联物的 Ex/Em：493/516 nm
• 消光系数：73,000 cm-1M-1
• 光谱相似染料：FITC、GFP
•分子量：640.61

细胞示踪与追踪应用
Alexa Fluor™ 尸胺因明亮、小巧且具有水溶性而可作为出色的荧光极性示踪剂。由于它们含有醛固定官能团，可通过甲醛或戊二醛处理固定在细胞中。它们可通过显微注射、从膜片移液管注入或通过我们的 Influx™ 胞饮细胞上样试剂所诱导的摄取进入细胞。了解更多关于细胞示踪与追踪的信息。

蛋白标记应用
Alexa Fluor™ 尸胺可用作使用偶联试剂（如碳二亚胺）向羧酸添加荧光标记的反应性分子；它们不会自发与溶液中的羧酸发生反应。然而，它们可以自发与常见的胺反应性官能团（包括琥珀酰亚胺酯和异硫氰酸盐）发生反应。含胺 Alexa Fluor™ 尸胺也可用于通过酶催化酰胺基转移反应标记某些蛋白和肽中的谷氨酰胺残基。

了解更多关于蛋白和抗体标记的信息
我们提供多种可供选择的 Molecular Probes™ 抗体和蛋白标记试剂盒，以适应您的起始材料和实验设置。参见我们的抗体标记试剂盒或使用我们的标记化学选择工具进行其他选择。欲了解有关我们标记试剂盒的更多信息，请参阅 Molecular Probes™ 手册中第 1.2 节—蛋白和核酸标记试剂盒。

我们还’可为您定制偶联物
如果您’无法在我们的在线目录中找到’想要的产品，我们还’可为您定制抗体或蛋白偶联物。我们的定制偶联服务是高效和保密的，我们保证我们的工作质量。我们经过 ISO 9001:2000 认证。

相关产品
DMSO（二甲亚砜）(D12345)
0.5-1 mg 用抗体偶联物纯化试剂盒 (A33086)
20-50 µg 用抗体偶联物纯化试剂盒 (A33087)
50-100 µg 用抗体偶联物纯化试剂盒 (A33088)
仅供科研使用。不可用于诊断程序。
规格
化学反应性羧酸、酮、醛
发射516 nm
激发493 nm
标签或染料Alexa Fluor™ 488
分子量640.61 g/mol
产品类型尸胺
数量1 mg
反应一部分胺、尸胺
运输条件室温
标签类型Alexa Fluor 染料
产品线Alexa Fluor
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (8)

引用和文献
Abstract
Optical properties of Alexa 488 and Cy5 immobilized on a glass surface.
Authors:Wang L, Gaigalas AK, Reipa V
Journal:Biotechniques
PubMed ID:15679095
'The absorption and emission spectra were measured for Cy5 and Alexa 488 fluorophores confined on a glass surface. The data were obtained using fluorometry and spectroscopic ellipsometry. Red shifts of the surface-immobilized fluorophore absorption spectra relative to the fluorophore spectra in aqueous solution were observed using both methods. We interpret ... More
Visualization of galectin-3 oligomerization on the surface of neutrophils and endothelial cells using fluorescence resonance energy transfer.
Authors:Nieminen J, Kuno A, Hirabayashi J, Sato S
Journal:J Biol Chem
PubMed ID:17082191
'Galectin-3, a member of the galectin family of carbohydrate binding proteins, is widely expressed, particularly in cells involved in the immune response. Galectin-3 has also been indicated to play a role in various biological activities ranging from cell repression to cell activation and adhesion and has, thus, been recognized as ... More
The obligatory role of the actin cytoskeleton on inward remodeling induced by dithiothreitol activation of endogenous transglutaminase in isolated arterioles.
Authors:Castorena-Gonzalez JA, Staiculescu MC, Foote CA, Polo-Parada L, Martinez-Lemus LA,
Journal:
PubMed ID:24337457
Inward remodeling is the most prevalent structural change found in the resistance arteries and arterioles of hypertensive individuals. Separate studies have shown that the inward remodeling process requires transglutaminase activation and the polymerization of actin. Therefore, we hypothesize that inward remodeling induced via endogenous transglutaminase activation requires and depends on ... More
Autophagy and protein kinase C are required for cardioprotection by sulfaphenazole.
Authors:Huang C, Liu W, Perry CN, Yitzhaki S, Lee Y, Yuan H, Tsukada YT, Hamacher-Brady A, Mentzer RM, Gottlieb RA,
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:20008275
Previously, we showed that sulfaphenazole (SUL), an antimicrobial agent that is a potent inhibitor of cytochrome P4502C9, is protective against ischemia-reperfusion (I/R) injury (Ref. 15). The mechanism, however, underlying this cardioprotection, is largely unknown. With evidence that activation of autophagy is protective against simulated I/R in HL-1 cells, and evidence ... More
Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution.
Authors:Huang B, Jones SA, Brandenburg B, Zhuang X,
Journal:Nat Methods
PubMed ID:19029906
The ability to directly visualize nanoscopic cellular structures and their spatial relationship in all three dimensions will greatly enhance our understanding of molecular processes in cells. Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions. To facilitate ... More
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