Visualization of galectin-3 oligomerization on the surface of neutrophils and endothelial cells using fluorescence resonance energy transfer.
Authors:Nieminen J, Kuno A, Hirabayashi J, Sato S
Journal:J Biol Chem
PubMed ID:17082191
'Galectin-3, a member of the galectin family of carbohydrate binding proteins, is widely expressed, particularly in cells involved in the immune response. Galectin-3 has also been indicated to play a role in various biological activities ranging from cell repression to cell activation and adhesion and has, thus, been recognized as ... More
SOI optical microring resonator with poly(ethylene glycol) polymer brush for label-free biosensor applications.
Authors:De Vos K, Girones J, Popelka S, Schacht E, Baets R, Bienstman P,
Journal:Biosens Bioelectron
PubMed ID:19200711
Label-free monitoring of biomolecular interactions has become of key importance for the emerging proteomics field. Monitoring real time interaction kinetics and high throughput screening of complex samples is of major importance for a variety of applications. We previously reported the use of Silicon-on-Insulator photonics microring resonators for cheap disposable biosensors ... More
Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution.
Authors:Huang B, Jones SA, Brandenburg B, Zhuang X,
Journal:Nat Methods
PubMed ID:19029906
The ability to directly visualize nanoscopic cellular structures and their spatial relationship in all three dimensions will greatly enhance our understanding of molecular processes in cells. Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions. To facilitate ... More
In vivo imaging of membrane-associated glycans in developing zebrafish.
Glycans are attractive targets for molecular imaging but have been inaccessible because of their incompatibility with genetically encoded reporters. We demonstrated the noninvasive imaging of glycans in live developing zebrafish, using a chemical reporter strategy. Zebrafish embryos were treated with an unnatural sugar to metabolically label their cell-surface glycans with ... More