Alexa Fluor™ 647 尸胺
Alexa Fluor™ 647 尸胺
引用和文献 (9)
Invitrogen™

Alexa Fluor™ 647 尸胺

Alexa Fluor™ 647 尸胺在作为一种极性示踪剂和作为通过羧酸部分标记蛋白的反应性染料时非常有用。Alexa Fluor™ 647 是一种明亮的远红荧光染料，其激发光谱非常适合于 633 nm了解更多信息
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货号数量
A30679
又称 A-30679
1 mg
货号 A30679
又称 A-30679
价格（CNY)
5,554.00
Each
添加至购物车
数量:
1 mg
价格（CNY)
5,554.00
Each
添加至购物车
Alexa Fluor™ 647 尸胺在作为一种极性示踪剂和作为通过羧酸部分标记蛋白的反应性染料时非常有用。Alexa Fluor™ 647 是一种明亮的远红荧光染料，其激发光谱非常适合于 633 nm 激光谱线。Alexa Fluor™ 647 染料用于成像和流式细胞分析中稳定信号的生成，具有水溶性和 pH 值不敏感性 (pH 4 - pH 10)。除反应性染料配方外，我们还提供可与多种抗体、肽、蛋白、示踪剂和扩增底物偶联并且针对细胞标记和检测进行优化的 Alexa Fluor™ 647 染料（了解更多信息）。

关于该 AlexaFluor™ 尸胺的详细信息：

•荧光基团标记：Alexa Fluor™ 647 染料
•反应性基团：尸胺
•反应性：羧酸、醛和酮（以及通过酶催化酰胺基转移反应产生的谷氨酰胺残基）
•偶联物的 Ex/Em：651/672 nm
• 消光系数：245,000 cm-1M-1
• 光谱相似染料：APC、Cy5
• 分子量：∼1000

细胞示踪与追踪应用
Alexa Fluor™ 尸胺因明亮、小巧且具有水溶性而可作为出色的荧光极性示踪剂。由于它们含有醛固定官能团，可通过甲醛或戊二醛处理固定在细胞中。它们可通过显微注射、从膜片移液管注入或通过我们的 Influx™ 胞饮细胞上样试剂所诱导的摄取进入细胞。了解更多关于细胞示踪与追踪的信息。

蛋白标记应用
Alexa Fluor™ 尸胺可用作使用偶联试剂（如碳二亚胺）向羧酸添加荧光标记的反应性分子；它们不会自发与溶液中的羧酸发生反应。然而，它们可以自发与常见的胺反应性官能团（包括琥珀酰亚胺酯和异硫氰酸盐）发生反应。含胺 Alexa Fluor™ 尸胺也可用于通过酶催化酰胺基转移反应标记某些蛋白和肽中的谷氨酰胺残基。

了解更多关于蛋白和抗体标记的信息
我们提供多种可供选择的 Molecular Probes™ 抗体和蛋白标记试剂盒，以适应您的起始材料和实验设置。参见我们的抗体标记试剂盒或使用我们的标记化学选择工具进行其他选择。欲了解有关我们标记试剂盒的更多信息，请参阅 Molecular Probes™ 手册中第 1.2 节—蛋白和核酸标记试剂盒。

我们还’可为您定制偶联物
如果您’无法在我们的在线目录中找到’想要的产品，我们还’可为您定制抗体或蛋白偶联物。我们的定制偶联服务是高效和保密的，我们保证我们的工作质量。我们经过 ISO 9001:2000 认证。

相关产品
DMSO（二甲亚砜）(D12345)
0.5-1 mg 用抗体偶联物纯化试剂盒 (A33086)
20-50 µg 用抗体偶联物纯化试剂盒 (A33087)
50-100 µg 用抗体偶联物纯化试剂盒 (A33088)
仅供科研使用。不可用于诊断程序。
规格
化学反应性羧酸、酮、醛
发射672 nm
激发651 nm
标签或染料Alexa Fluor™ 647
产品类型尸胺
数量1 mg
反应一部分胺、尸胺
运输条件室温
标签类型Alexa Fluor 染料
产品线Alexa Fluor
Unit SizeEach
内容与储存
在冷冻冰箱(-5°C 至 -30°C)中避光储存。

引用和文献 (9)

引用和文献
Abstract
Death-receptor activation halts clathrin-dependent endocytosis.
Authors:Austin CD, Lawrence DA, Peden AA, Varfolomeev EE, Totpal K, De Mazière AM, Klumperman J, Arnott D, Pham V, Scheller RH, Ashkenazi A
Journal:Proc Natl Acad Sci U S A
PubMed ID:16801533
'Endocytosis is crucial for various aspects of cell homeostasis. Here, we show that proapoptotic death receptors (DRs) trigger selective destruction of the clathrin-dependent endocytosis machinery. DR stimulation induced rapid, caspase-mediated cleavage of key clathrin-pathway components, halting cellular uptake of the classic cargo protein transferrin. DR-proximal initiator caspases cleaved the clathrin ... More
A modular IgG-scFv bispecific antibody topology.
Authors:Orcutt KD, Ackerman ME, Cieslewicz M, Quiroz E, Slusarczyk AL, Frangioni JV, Wittrup KD,
Journal:Protein Eng Des Sel
PubMed ID:20019028
'Here we present a bispecific antibody (bsAb) format in which a disulfide-stabilized scFv is fused to the C-terminus of the light chain of an IgG to create an IgG-scFv bifunctional antibody. When expressed in mammalian cells and purified by one-step protein A chromatography, the bsAb retains parental affinities of each ... More
Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection.
Authors:Janjic JM, Srinivas M, Kadayakkara DK, Ahrens ET,
Journal:J Am Chem Soc
PubMed ID:18266363
We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes (FITC, Alexa647 and BODIPy-TR), mixed with pluronic F68 and linear polyethyleneimine (PEI), and emulsified by microfluidization. Prepared nanoemulsions (<200 ... More
Development and in vivo efficacy of targeted polymeric inflammation-resolving nanoparticles.
Authors:Kamaly N, Fredman G, Subramanian M, Gadde S, Pesic A, Cheung L, Fayad ZA, Langer R, Tabas I, Farokhzad OC,
Journal:Proc Natl Acad Sci U S A
PubMed ID:23533277
Excessive inflammation and failed resolution of the inflammatory response are underlying components of numerous conditions such as arthritis, cardiovascular disease, and cancer. Hence, therapeutics that dampen inflammation and enhance resolution are of considerable interest. In this study, we demonstrate the proresolving activity of sub-100-nm nanoparticles (NPs) containing the anti-inflammatory peptide ... More
Adsorbed proteins influence the biological activity and molecular targeting of nanomaterials.
Authors:Dutta D, Sundaram SK, Teeguarden JG, Riley BJ, Fifield LS, Jacobs JM, Addleman SR, Kaysen GA, Moudgil BM, Weber TJ,
Journal:Toxicol Sci
PubMed ID:17709331
The possible combination of specific physicochemical properties operating at unique sites of action within cells and tissues has led to considerable uncertainty surrounding nanomaterial toxic potential. We have investigated the importance of proteins adsorbed onto the surface of two distinct classes of nanomaterials (single-walled carbon nanotubes [SWCNTs]; 10-nm amorphous silica) ... More
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