TaqPath™ ProAmp™ Multiplex Master Mix, 1 x 10 mL - FAQs

查看更多产品信息 TaqPath™ ProAmp™ Multiplex Master Mix - FAQs (A30870, A30873, A30874, A30868, A30869)

3 个常见问题解答

What Applied Biosystems reagents and instruments can I use with TaqMan SNP Genotyping Assays?

The TaqPath ProAmp Master Mix or TaqPath ProAmp Multiplex Master Mix is recommended for TaqMan SNP Genotyping Assays. Additional PCR master mixes that can be used as well, include the TaqMan Universal PCR Master Mix, No AmpErase, TaqMan Universal PCR Master Mix, TaqMan Universal Master Mix II, TaqMan genotyping Master mix, and GTXpress Master Mix. The TaqMan SNP Genotyping Assays can be used on all the real-time PCR instruments from Applied Biosystems, including 7000, 7300, 7500, 7500 Fast, 7700, StepOne, StepOnePlus, 7900HT, ViiA7, and QuantStudio systems.

I need to multiplex but cannot use ROX. What options do I have?

For multiplex assays that include reporter dyes overlapping the ROX channel (i.e. JUN), we recommend using one of our optimized multiplex master mixes that include the passive reference dye, MUSTANG PURPLE. MUSTANG PURPLE is compatible with QuantStudio 5, 6, 7, and 12K Flex, 7500, 7500 Fast, and ViiA 7 real-time PCR instruments.

While not ideal, we also offer master mixes that contain no passive reference dyes. For selection help for master mixes with no passive reference, please contact Technical Support at techsupport@thermofisher.com.

What master mix should I use for multiplexing in my qPCR experiments?

The choice of master mix depends upon the number of assays in your reaction, the application, and instrument compatibility. Please refer to our real-time PCR master mixes and instrument compatibility chart (https://assets.thermofisher.com/TFS-Assets/LSG/brochures/master-mix-instrument-compatibility.pdf) prior to selecting one of the master mixes below:

Gene Expression Assays:
- Single, duplex, and triplex assays: TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mix
- Multiplexing up to 4 assays: TaqMan Multiplex Master Mix or TaqPath 1-Step Multiplex Master Mix

SNP Genotyping Assays:
- Single SNP Genotyping assay: TaqPath ProAmp Master Mix
- Duplex SNP Genotyping assays: TaqPath ProAmp Multiplex Master Mix, TaqMan Multiplex Master Mix

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.