Ion AmpliSeq™ Direct FFPE DNA Kit, 96 preps - FAQs

View additional product information for Ion AmpliSeq™ Direct FFPE DNA Kit - FAQs (A31133, A31136)

10 product FAQs found

With the Oncomine BRCA Research Assay, which method do you recommend for isolating gDNA?

We recommend the following DNA isolation kits:

- RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Cat. No. AM1975)
- Ion Ampliseq Direct FFPE DNA Kit (Cat. Nos. A31133, A31136)
- MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881)
- PureLink Genomic DNA Mini Kit (Cat. Nos, K182000, K182001, K182002)

I am planning to use the Ion AmpliSeq Direct FFPE Kit. Can I continuously store the original FFPE slide after sample collection if only a section of the sample was used?

No, transfer solution will continue to penetrate the entire FFPE slide.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion AmpliSeq Direct FFPE Kit be used to make manual and automated Ion AmpliSeq libraries?

Yes, FFPE DNA derived from the Ion AmpliSeq Direct FFPE Kit kit can be used with both the manual and automated Ion AmpliSeq library processes.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion AmpliSeq Direct FFPE DNA workflow be automated?

The Ion AmpliSeq Direct FFPE DNA workflow cannot be automated because the slide collection step is manual.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long can the Ion AmpliSeq Direct FFPE DNA extract be stored?

We have data for storage at -20 degrees C for 3 months.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion AmpliSeq Direct FFPE DNA extract be quantified?

Yes, we recommend using the Qubit Fluorometer as an optional method of quantitation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Do you have a video showing how to use the Ion AmpliSeq Direct FFPE Kit?

Yes, please see here (https://youtu.be/x8Rb50oHRug).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the shelf life of the Ion AmpliSeq Direct FFPE Kit?

The shelf life of the Ion AmpliSeq Direct FFPE Kit is 3 years from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Ion AmpliSeq Direct FFPE Kit be used in conjunction with large AmpliSeq panels, such as the Oncomine Comprehensive Panel?

Yes, the Ion Ampliseq Direct FFPE Kit has been tested on large Ampliseq panels, with varying amounts of FFPE DNA input, resulting in high coverage uniformity.

How long does it take to complete DNA preparation using the Ion AmpliSeq Direct FFPE Kit?

It takes 30 minutes to complete DNA preparation using the Ion AmpliSeq Direct FFPE Kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.