Fetal Bovine Serum, qualified, New Zealand
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Fetal Bovine Serum, qualified, New Zealand
Gibco™

Fetal Bovine Serum, qualified, New Zealand

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Important Update: We've recently introduced Premium Plus Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Certified US origin, New Zealand origin, and Qualified Australia origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.
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Catalog NumberQuantityFormat
A316090150 mLOne Shot
A316090210 x 50 mLOne Shot
10091148500 mLBottle
我们升级后的 Premium Plus 胎牛血清 (FBS)，在维持一贯稳定性、生产、储存与运输工艺的基础上，进一步优化了产品规格，为科研工作提供更可靠的支持。
Catalog number A3160901
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Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Premium (Secure)
  • Origin: New Zealand
  • Endotoxin level: ≤5 EU/mL
  • Hemoglobin level: ≤30 mg/dL (levels routinely ≤25 mg/dL)
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
DescriptionFetal Bovine Serum, qualified, One Shot™ format, New Zealand
Endotoxin Concentration≤5 EU/mL
Hemoglobin Concentration≤30 mg/dL
Product LineOne Shot™
Purity or Quality GradeQualified
Shipping ConditionFrozen
SpeciesBovine
AgeFetal
Country of OriginNew Zealand
FormLiquid
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity50 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

Why would I heat inactivate serum?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat inactivated serum is recommended include immunological studies and culturing ES cells, insect cells, and smooth muscle cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I heat inactivate serum?

Heat at 56 degrees C in a water bath for 30 minutes. Swirl bottle every 10 minutes or so. For accuracy, use a second bottle of similar size and add the equivalent volume of water to this bottle. Place a thermometer in this control bottle in order to see when 56 degrees C is reached. Set your timer for 30 minutes at this point.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

View all Fetal Bovine Serum, qualified, New Zealand FAQs