Identification of nicotinic acetylcholine receptor recycling and its role in maintaining receptor density at the neuromuscular junction in vivo.
AuthorsBruneau E, Sutter D, Hume RI, Akaaboune M
JournalJ Neurosci
PubMed ID16251443
'In the CNS, receptor recycling is critical for synaptic plasticity; however, the recycling of receptors has never been observed at peripheral synapses. Using a novel imaging technique, we show here that nicotinic acetylcholine receptors (AChRs) recycle into the postsynaptic membrane of the neuromuscular junction. By sequentially labeling AChRs with biotin-bungarotoxin ... More
Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library.
'A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead screening and flow-cytometric sorting. The yeast library can be amplified 10(10)-fold without measurable loss of clonal diversity, allowing its effectively indefinite expansion. The ... More
Fluorescence biosensing strategy based on energy transfer between fluorescently labeled receptors and a metallic surface.
AuthorsPérez-Luna VH, Yang S, Rabinovich EM, Buranda T, Sklar LA, Hampton PD, López GP
JournalBiosens Bioelectron
PubMed ID11742737
'A new fluorescence-based biosensor is presented. The biosensing scheme is based on the fact that a fluorophore in close proximity to a metal film (<100 A) experiences strong quenching of fluorescence and a dramatic reduction in the lifetime of the excited state. By immobilizing the analyte of interest (or a ... More
Chromatin loop domain organization within the 4q35 locus in facioscapulohumeral dystrophy patients versus normal human myoblasts.
AuthorsPetrov A, Pirozhkova I, Carnac G, Laoudj D, Lipinski M, Vassetzky YS
JournalProc Natl Acad Sci U S A
PubMed ID16632607
Fascioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant neuromuscular disorder linked to partial deletion of integral numbers of a 3.3 kb polymorphic repeat, D4Z4, within the subtelomeric region of chromosome 4q. Although the relationship between deletions of D4Z4 and FSHD is well established, how this triggers the disease remains unclear. ... More