Vitronectin (VTN-N) Recombinant Human Protein, Truncated
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IMPORTANT UPDATE: Vitronectin (VTN-N) Recombinant Human Protein, Truncated, now available under new catalog numbers, A400457 and A400458. Catalog numbers A14700 and A31804 will be discontinued.
Vitronectin (VTN-N) Recombinant Human Protein, Truncated
Gibco™

Vitronectin (VTN-N) Recombinant Human Protein, Truncated

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs).
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Catalog NumberQuantity
A3180410 mL = 5 mg
A147001 mL = 0.5 mg
Catalog number A31804
Price (CNY)
8,749.90
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Ends: 31-Dec-2025
10,438.00
Save 1,688.10 (16%)
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Quantity:
10 mL = 5 mg
Price (CNY)
8,749.90
Online Exclusive
Ends: 31-Dec-2025
10,438.00
Save 1,688.10 (16%)
Each
Add to cart

Vitronectin (VTN-N) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8 Medium (Cat. No. A1517001), VTN-N has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines.

  • Proven to maintain pluripotency and normal growth characteristics in multiple PSC lines when used with Essential 8 Medium
  • Supports PSC growth for >50 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages
  • Supports human pluripotent stem cell attachment and survival better than wild-type vitronectin when used in conjunction with Essential 8 Medium
  • Optimized—designed in the laboratory of James Thomson for use with Essential 8 Medium
  • Defined—for reduced variability in your PSC cultures
  • Supports human pluripotent stem cell attachment and survival better than wild type vitronectin
  • Optimal environment for feeder-free pluripotent stem cell culture
  • Defined, recombinant human protein that reduces variability in your PSC cultures compared to human-plasma derived vitronectin and standard basement membrane extracts (BMEs)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration500 μg/mL
Expression SystemE. coli
Protein FormRecombinant
Protein SubtypeVitronectin
Purity or Quality GradeCell Culture Grade, Endotoxin-Screened
Quantity10 mL = 5 mg
Research CategoryStem Cell Research
Shipping ConditionDry Ice
Volume (Metric)10 mL
For Use With (Application)Stem Cell Culture
FormLiquid
RecombinantRecombinant
SpeciesHuman
Unit SizeEach
Contents & Storage
Store at -80°C and protect from light.

Frequently asked questions (FAQs)

What substrate should I use with the PSC Definitive Endoderm Induction Kit?

We recommend using Vitronectin (VTN-N) Recombinant Human Protein, Truncated (Cat. No. A14700), but Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (Cat. No. A1413301 or Cat. No. A1413302) can also be used.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can pluripotent stem cells (PSCs) cryopreserved in a different culture condition be thawed and subsequently cultured in Gibco Essential 8 Medium and on vitronectin (VTN-N)?

Yes. PSCs cryopreserved from cultures of mTeSR Medium and BD Matrigel Basement Membrane Matrix may be thawed into Gibco Essential 8 Medium and plated on VTN-N. Certain lines may benefit from thawing into the medium and substrate they were growing in at the time of cryopreservation. Then at the next passage, use EDTA to passage the cells into Gibco Essential 8 Medium and VTN-N.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use enzymes such as dispase and collagenase for passaging cells cultured in Gibco Essential 8 Medium and on VTN-N?

Enzymes such as dispase and collagenase do not work well with cells cultured in Gibco Essential 8 Medium on VTN-N. Use of these enzymes for passaging cells results in compromised viability and attachment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended passaging method to use with Gibco Essential 8 Medium and on Gibco Vitronectin (VTN-N)?

Cells cultured in Gibco Essential 8 Medium and VTN-N need to be passaged with EDTA.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What will my cells look like growing in Gibco Essential 8 Medium and on Gibco Vitronectin (VTN-N)?

You should expect to see normal pluripotent stem cell (PSC) morphology. The expected morphology of PSCs is demonstrated specifically by tightly packed colonies with defined borders and a high nucleus-to-cytoplasm ratio.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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